PMID- 31442607
OWN - NLM
STAT- MEDLINE
DCOM- 20200420
LR  - 20200420
IS  - 1878-5875 (Electronic)
IS  - 1357-2725 (Linking)
VI  - 115
DP  - 2019 Oct
TI  - GATA-6 transcriptionally inhibits Shh to repress cell proliferation and migration 
      in lung squamous cell carcinoma.
PG  - 105591
LID - S1357-2725(19)30168-2 [pii]
LID - 10.1016/j.biocel.2019.105591 [doi]
AB  - GATA-6 is a transcription factor that participates in cell lineage 
      differentiation and organogenesis in many tissue types. The abnormal expression 
      of GATA-6 is associated with the development of diverse cancers. GATA-6 acts as 
      an oncogene or tumor suppressor based on tumor origin. Here, we investigated the 
      effects of GATA-6 on lung squamous cell carcinoma (LSCC). We found that GATA-6 
      was significantly reduced in LSCC tissues compared with the paired normal 
      tissues. The overexpression of GATA-6 inhibited LSCC cell proliferation and 
      migration. Importantly, a luciferase reporter assay and chromatin 
      immunoprecipitation assay demonstrated that GATA-6 negatively regulated the 
      expression of sonic hedgehog (Shh) by directly binding to its promoter region. 
      Furthermore, N-Shh stimulation rescued the inhibition of LSCC cell proliferation 
      and migration upon GATA-6 overexpression. Thus, GATA-6 inhibited the 
      proliferation and migration of LSCC cells by transcriptionally inhibiting the 
      expression of Shh, indicating that targeting GATA-6 may be a potential approach 
      for LSCC therapy.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Xu, Linlin
AU  - Xu L
AD  - Center for Experimental Medicine, The First Affiliated Hospital of Nanchang 
      University, Nanchang, Jiangxi 330006, China; Jiangxi Key Laboratory of Molecular 
      Diagnostics and Precision Medicine, Nanchang, Jiangxi 330006, China.
FAU - Deng, Suyue
AU  - Deng S
AD  - Center for Experimental Medicine, The First Affiliated Hospital of Nanchang 
      University, Nanchang, Jiangxi 330006, China; Jiangxi Key Laboratory of Molecular 
      Diagnostics and Precision Medicine, Nanchang, Jiangxi 330006, China.
FAU - Xiong, Huanting
AU  - Xiong H
AD  - Center for Experimental Medicine, The First Affiliated Hospital of Nanchang 
      University, Nanchang, Jiangxi 330006, China; School of Pharmacy, Nanchang 
      University, Nanchang, Jiangxi 330006, China; Jiangxi Key Laboratory of Molecular 
      Diagnostics and Precision Medicine, Nanchang, Jiangxi 330006, China.
FAU - Shi, Wei
AU  - Shi W
AD  - Center for Experimental Medicine, The First Affiliated Hospital of Nanchang 
      University, Nanchang, Jiangxi 330006, China; School of Pharmacy, Nanchang 
      University, Nanchang, Jiangxi 330006, China; Jiangxi Key Laboratory of Molecular 
      Diagnostics and Precision Medicine, Nanchang, Jiangxi 330006, China.
FAU - Luo, Shiwen
AU  - Luo S
AD  - Center for Experimental Medicine, The First Affiliated Hospital of Nanchang 
      University, Nanchang, Jiangxi 330006, China; Jiangxi Key Laboratory of Molecular 
      Diagnostics and Precision Medicine, Nanchang, Jiangxi 330006, China.
FAU - Chen, Limin
AU  - Chen L
AD  - Center for Experimental Medicine, The First Affiliated Hospital of Nanchang 
      University, Nanchang, Jiangxi 330006, China; Jiangxi Key Laboratory of Molecular 
      Diagnostics and Precision Medicine, Nanchang, Jiangxi 330006, China. Electronic 
      address: liminchen@ncu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190820
PL  - Netherlands
TA  - Int J Biochem Cell Biol
JT  - The international journal of biochemistry & cell biology
JID - 9508482
RN  - 0 (GATA6 Transcription Factor)
RN  - 0 (Hedgehog Proteins)
RN  - 0 (SHH protein, human)
SB  - IM
MH  - Carcinoma, Squamous Cell/*pathology
MH  - *Cell Movement
MH  - Cell Proliferation
MH  - GATA6 Transcription Factor/*metabolism
MH  - Gene Expression Regulation, Neoplastic
MH  - HEK293 Cells
MH  - Hedgehog Proteins/*genetics
MH  - Humans
MH  - Lung Neoplasms/*pathology
MH  - *Transcription, Genetic
OTO - NOTNLM
OT  - GATA-6
OT  - Lung squamous carcinoma (LSCC)
OT  - Migration
OT  - Proliferation
OT  - Sonic hedgehog (Shh)
EDAT- 2019/08/24 06:00
MHDA- 2020/04/21 06:00
CRDT- 2019/08/24 06:00
PHST- 2019/04/16 00:00 [received]
PHST- 2019/07/19 00:00 [revised]
PHST- 2019/08/19 00:00 [accepted]
PHST- 2019/08/24 06:00 [pubmed]
PHST- 2020/04/21 06:00 [medline]
PHST- 2019/08/24 06:00 [entrez]
AID - S1357-2725(19)30168-2 [pii]
AID - 10.1016/j.biocel.2019.105591 [doi]
PST - ppublish
SO  - Int J Biochem Cell Biol. 2019 Oct;115:105591. doi: 10.1016/j.biocel.2019.105591. 
      Epub 2019 Aug 20.