PMID- 31445135
OWN - NLM
STAT- MEDLINE
DCOM- 20200218
LR  - 20200218
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 245
DP  - 2019 Dec 5
TI  - Potential toxicity effects of Anastatica hierochuntica aqueous extract on 
      prenatal development of Sprague-Dawley rats.
PG  - 112180
LID - S0378-8741(18)34971-7 [pii]
LID - 10.1016/j.jep.2019.112180 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Anastatica hierochuntica (A. hierochuntica) is a 
      plant consumed in folk medicine for the treatment of reproductive system related 
      problems and metabolic disorders. It is of concern that the herb is commonly 
      consumed by pregnant women towards the end of pregnancy to ease the process of 
      labour, despite the lack of studies evaluating its safety. AIM OF THIS STUDY: 
      This study aimed to investigate the potential toxicity effects of A. 
      hierochuntica in pregnant Sprague-Dawley rats and their developing foetuses. 
      MATERIALS AND METHODS: Experiments were conducted in accordance to the 
      Organisation for Economic Co-operation and Development guideline 414. Animals 
      were randomly divided into four groups (n = 10 females per group): negative 
      control (received the vehicle only), experimental animals received 250, 500, and 
      1000 mg/kg A. hierochuntica aqueous extracts (AHAE), respectively. Treatment was 
      administered daily by oral gavage from gestational day (GD) 6-20, and caesarian 
      section performed on GD21. RESULTS: There were significant reduction in the 
      corrected maternal weight gain of dams and body weight of foetuses in the lowest 
      and highest dose of AHAE-treated animals compared to the control. These findings 
      were associated with the increase in anogenital distance index and multiple 
      congenital anomalies observed in some of the offspring. On the other hand, rats 
      treated with 500 mg/kg showed higher embryonic survival rate with absence of 
      significant treatment-related effect. CONCLUSION: Findings showed that highest 
      and lowest doses of AHAE have prenatal toxicity effects in SD rats. Therefore, 
      AHAE is potentially harmful to the developing foetuses especially when consumed 
      during the period of implantation and organogenesis. As for the rats treated with 
      500 mg/kg AHAE, there was no significant treatment-related effect. Hence, we 
      postulate that this finding suggests that the disruption on the hormonal 
      regulation could have been compensated by negative feedback response. The 
      compensated effects of AHAE at 500 mg/kg and the presence of lowest observed 
      adverse effect level (LOAEL) at 250 mg/kg has resulted in a non-monotonous dose 
      response curve (NMDRC), which complicates the determination of the value of 
      no-observed-adverse effect level (NOAEL).
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Md Zin, Siti Rosmani
AU  - Md Zin SR
AD  - Department of Anatomy, Faculty of Medicine, University of Malaya, 50603, Kuala 
      Lumpur, Malaysia.
FAU - Kassim, Normadiah M
AU  - Kassim NM
AD  - Department of Anatomy, Faculty of Medicine, University of Malaya, 50603, Kuala 
      Lumpur, Malaysia. Electronic address: normadiah_mk@um.edu.my.
FAU - Mohamed, Zahurin
AU  - Mohamed Z
AD  - Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603, 
      Kuala Lumpur, Malaysia.
FAU - Fateh, Abdulmannan H
AU  - Fateh AH
AD  - Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603, 
      Kuala Lumpur, Malaysia.
FAU - Alshawsh, Mohammed A
AU  - Alshawsh MA
AD  - Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603, 
      Kuala Lumpur, Malaysia.
LA  - eng
PT  - Journal Article
DEP - 20190822
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Abnormalities, Drug-Induced/*etiology
MH  - Abnormalities, Multiple/*chemically induced
MH  - Animals
MH  - *Brassicaceae
MH  - Embryo Implantation
MH  - Female
MH  - Fetal Development/*drug effects
MH  - Male
MH  - Plant Extracts/*toxicity
MH  - Pregnancy
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Anastatica hierochuntica
OT  - Prenatal toxicity
OT  - Reproductive parameters
OT  - Sanggul Fatimah
OT  - Sprague-Dawley rats
EDAT- 2019/08/25 06:00
MHDA- 2020/02/19 06:00
CRDT- 2019/08/25 06:00
PHST- 2018/12/25 00:00 [received]
PHST- 2019/08/13 00:00 [revised]
PHST- 2019/08/21 00:00 [accepted]
PHST- 2019/08/25 06:00 [pubmed]
PHST- 2020/02/19 06:00 [medline]
PHST- 2019/08/25 06:00 [entrez]
AID - S0378-8741(18)34971-7 [pii]
AID - 10.1016/j.jep.2019.112180 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2019 Dec 5;245:112180. doi: 10.1016/j.jep.2019.112180. Epub 
      2019 Aug 22.