PMID- 31447851
OWN - NLM
STAT- MEDLINE
DCOM- 20201021
LR  - 20201021
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 10
DP  - 2019
TI  - Screening and Identification of a Chicken Dendritic Cell Binding Peptide by Using 
      a Phage Display Library.
PG  - 1853
LID - 10.3389/fimmu.2019.01853 [doi]
LID - 1853
AB  - Dendritic cells (DCs), as antigen-presenting cells, can initiate adaptive immune 
      responses efficiently. Although the DC-targeting strategy has attracted more 
      attention, relevant studies on chicken are rare. Here, specific chicken bone 
      marrow DC-binding peptides were selected using a phage display peptide library 
      and confirmed through ELISA, flow cytometry, fluorescence microscopy, and laser 
      confocal microscopy. The peptide candidate SPHLHTSSPWER, named SP, was fused to 
      the infectious bursal disease virus (IBDV) structural protein and protective 
      antigen VP2. In vitro, the expression of DC markers (CD80, CD83, CD86, DEC205, 
      and MHCII) and some cytokines (IFN-gamma, IL-12, TNF-alpha, IL-1beta, IL-6, and CXCLi1) by 
      VP2-SP-stimulated DCs was significantly higher than that by DCs treated with the 
      VP2-control peptide at 4 h (p < 0.001). In addition, an oral vaccine targeting 
      DCs was generated using chicken-borne Lactobacillus saerimneri M11 (L. sae M11) 
      to deliver VP2 fused with SP. Anti-IBDV mucosal and humoral immune responses were 
      induced efficiently via oral administration, resulting in higher protective 
      efficacy in the VP2-SP group than the VP2 group. Therefore, chicken DC targeting 
      of IBDV protective antigen VP2 delivered by L. sae provides effective immune 
      protection in chicken. Our study may promote research on the DC-targeting 
      strategy to enhance the effectiveness of chicken vaccines.
FAU - Ma, Sunting
AU  - Ma S
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
AD  - Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical 
      Development, Harbin, China.
AD  - Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of 
      Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural 
      Sciences, Nanjing, China.
FAU - Qiao, Xinyuan
AU  - Qiao X
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
AD  - Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical 
      Development, Harbin, China.
FAU - Xu, Yigang
AU  - Xu Y
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
AD  - Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical 
      Development, Harbin, China.
FAU - Wang, Li
AU  - Wang L
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Zhou, Han
AU  - Zhou H
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Jiang, Yanping
AU  - Jiang Y
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Cui, Wen
AU  - Cui W
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Huang, Xuewei
AU  - Huang X
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Wang, Xiaona
AU  - Wang X
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Tang, Lijie
AU  - Tang L
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
AD  - Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical 
      Development, Harbin, China.
FAU - Li, Yijing
AU  - Li Y
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
AD  - Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical 
      Development, Harbin, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190806
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Peptide Library)
RN  - 0 (Vaccines, Synthetic)
RN  - 0 (Viral Structural Proteins)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Bacteriophages/metabolism
MH  - Chick Embryo
MH  - Chickens
MH  - Dendritic Cells/*immunology
MH  - Infectious bursal disease virus/*immunology
MH  - *Peptide Library
MH  - Vaccines, Synthetic/immunology
MH  - Viral Structural Proteins/immunology
MH  - Viral Vaccines/*immunology
PMC - PMC6691127
OTO - NOTNLM
OT  - chicken dendritic cells-binding peptides
OT  - infectious bursal disease virus
OT  - oral immunization
OT  - probiotic bacteria
OT  - vaccine delivery
EDAT- 2019/08/27 06:00
MHDA- 2020/10/22 06:00
PMCR- 2019/01/01
CRDT- 2019/08/27 06:00
PHST- 2019/04/11 00:00 [received]
PHST- 2019/07/23 00:00 [accepted]
PHST- 2019/08/27 06:00 [entrez]
PHST- 2019/08/27 06:00 [pubmed]
PHST- 2020/10/22 06:00 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2019.01853 [doi]
PST - epublish
SO  - Front Immunol. 2019 Aug 6;10:1853. doi: 10.3389/fimmu.2019.01853. eCollection 
      2019.