PMID- 31448543
OWN - NLM
STAT- MEDLINE
DCOM- 20200601
LR  - 20200601
IS  - 2059-2310 (Electronic)
IS  - 2059-2302 (Linking)
VI  - 94
IP  - 6
DP  - 2019 Dec
TI  - MiR-199a-3p modulates the function of dendritic cells involved in transplantation 
      tolerance by targeting CD86.
PG  - 493-503
LID - 10.1111/tan.13677 [doi]
AB  - Dendritic cells (DCs) are key components of the immune system, serving as 
      antigen-presenting cells to activate adaptive immunity. Whereas mature DCs 
      promote immune responses, immature DCs induce or maintain immunological tolerance 
      by downregulating T-cell responses. Therefore, DCs are potent antigen 
      (Ag)-presenting cells in the immune system. MicroRNAs are noncoding RNAs that 
      posttranscriptionally regulate mRNA by binding the 3'-untranslated region (UTR) 
      of these molecules, modulating their expression. Many recent studies have 
      suggested a potential role of miRNAs in DCs maturation and differentiation, but 
      the exact mechanisms governing this process are unclear. How and whether 
      miR-199a-3p affects DC maturation has not been investigated. Here, we found that 
      MiR-199a-3p levels are correlated with DC maturation, inflammatory cytokine 
      secretion, and PI3K/AKT/NF-kappaB signaling pathway activity. In addition, we 
      analyzed the stimulation of regulatory T-cells by DCs. Through this work, we 
      determined CD86 to be targeted by miR-199a-3p, thereby linking it to DC 
      maturation. miR-199a-3p therefore directly inhibits CD86 expression via 3'-UTR 
      targeting, subsequently prolonging allograft survival in a mouse heart 
      transplantation model. miR-199a-3p over-expression may therefore be a potential 
      therapeutic strategy for use in organ transplantation or patients with autoimmune 
      diseases.
CI  - (c) 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Xiong, Ali
AU  - Xiong A
AUID- ORCID: 0000-0003-3309-654X
AD  - Department of Medical Laboratory, The Central Hospital of Wuhan, TongJi Medical 
      College, Huazhong University of Science and Technology, WuHan, China.
FAU - Wang, Jing
AU  - Wang J
AD  - Department of Medical Laboratory, The Central Hospital of Wuhan, TongJi Medical 
      College, Huazhong University of Science and Technology, WuHan, China.
FAU - Mao, Xiao Li
AU  - Mao XL
AD  - Department of Medical Laboratory, The Central Hospital of Wuhan, TongJi Medical 
      College, Huazhong University of Science and Technology, WuHan, China.
FAU - Jiang, Yi
AU  - Jiang Y
AD  - Department of Medical Laboratory, The Central Hospital of Wuhan, TongJi Medical 
      College, Huazhong University of Science and Technology, WuHan, China.
FAU - Fan, Yue
AU  - Fan Y
AD  - Department of Medical Laboratory, The Central Hospital of Wuhan, TongJi Medical 
      College, Huazhong University of Science and Technology, WuHan, China.
LA  - eng
GR  - 31500729/National Natural Science Foundation of China/International
GR  - 20130142120056/US/United States/United States
GR  - 20130142120056/US/United States/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190903
PL  - England
TA  - HLA
JT  - HLA
JID - 101675570
RN  - 0 (3' Untranslated Regions)
RN  - 0 (B7-2 Antigen)
RN  - 0 (Cd86 protein, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn199 microRNA, mouse)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - Allografts/immunology
MH  - Animals
MH  - B7-2 Antigen/*genetics/metabolism
MH  - Cell Differentiation/genetics
MH  - Cell Proliferation/genetics
MH  - Cells, Cultured
MH  - Dendritic Cells/metabolism/*physiology
MH  - Gene Expression Regulation
MH  - Graft Survival/genetics/immunology
MH  - Heart Transplantation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/genetics/*physiology
MH  - Transplantation Tolerance/*genetics/*immunology
OTO - NOTNLM
OT  - CD86
OT  - NF-kappaB
OT  - dendritic cells
OT  - miR-199a-3p
OT  - transplantation
EDAT- 2019/08/27 06:00
MHDA- 2020/06/02 06:00
CRDT- 2019/08/27 06:00
PHST- 2019/02/02 00:00 [received]
PHST- 2019/08/06 00:00 [revised]
PHST- 2019/08/23 00:00 [accepted]
PHST- 2019/08/27 06:00 [pubmed]
PHST- 2020/06/02 06:00 [medline]
PHST- 2019/08/27 06:00 [entrez]
AID - 10.1111/tan.13677 [doi]
PST - ppublish
SO  - HLA. 2019 Dec;94(6):493-503. doi: 10.1111/tan.13677. Epub 2019 Sep 3.