PMID- 31450414
OWN - NLM
STAT- MEDLINE
DCOM- 20200203
LR  - 20200203
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 205
DP  - 2019 Dec 1
TI  - A multi-residue method for determination of 36 endocrine disrupting chemicals in 
      human serum with a simple extraction procedure in combination of UPLC-MS/MS 
      analysis.
PG  - 120144
LID - S0039-9140(19)30770-2 [pii]
LID - 10.1016/j.talanta.2019.120144 [doi]
AB  - Much attention has been paid to endocrine disrupting chemicals (EDCs) due to 
      their widespread presence in various environmental matrices, and endocrine 
      disrupting potential even at low concentrations. However, little is known about 
      the multiple EDCs exposure to human and related adverse effects on health, which 
      warrants a multi-residue method for simultaneous determination of EDCs in human 
      samples such as serum. In this study, we developed and validated a novel method 
      for determination of 36 EDCs (8 bisphenols, 7 parabens, 2 antimicrobials, 5 
      benzophenones, and 14 phthalate metabolites) in human serum with 
      ultra-performance liquid chromatography coupled to tandem mass spectrometry 
      (UPLC-MS/MS). Two extraction methods (liquid liquid extraction (LLE), and LLE 
      coupled with solid phase extraction (SPE) clean-up) were compared, and eleven 
      solvents and two SPE cartridges were optimized for extraction and clean-up 
      procedure, respectively. Recoveries of target compounds spiked in human serum at 
      three levels of concentrations (0.5, 2.5 and 10 ng/mL) ranged from 45.8% to 120%, 
      and the relative standard deviations (RSDs) were lower than 20%. The linearity of 
      the labeled dilution calibration curve was good with correlation coefficient 
      ranging from 0.995 to 0.999, and the limits of quantification (LOQs) were between 
      0.002 and 0.532 ng/mL. Low RSDs of intra-day (0.1-12.7%) and inter-day 
      (0.2-13.3%) revealed the accuracy and precision of the quantification. The method 
      was successfully applied to determine the target EDCs in human serum samples from 
      14 randomly selected individuals. The developed method is a promising method for 
      routine measurement of EDCs in human serum.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Wang, Yun
AU  - Wang Y
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Science, Beijing, 
      100085, China; College of Resources and Environment, University of Chinese 
      Academy Science, Beijing, 100049, China.
FAU - Li, Guoliang
AU  - Li G
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Science, Beijing, 
      100085, China.
FAU - Zhu, Qingqing
AU  - Zhu Q
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Science, Beijing, 
      100085, China.
FAU - Liao, Chunyang
AU  - Liao C
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Science, Beijing, 
      100085, China; College of Resources and Environment, University of Chinese 
      Academy Science, Beijing, 100049, China; Institute of Environment and Health, 
      Jianghan University, Wuhan, Hubei, 430056, China. Electronic address: 
      cyliao@rcees.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20190710
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Endocrine Disruptors)
SB  - IM
MH  - Blood Chemical Analysis/*methods
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Endocrine Disruptors/*blood/*isolation & purification
MH  - Healthy Volunteers
MH  - Humans
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Analysis
OT  - Endocrine disrupting chemicals
OT  - Human serum
OT  - Liquid liquid extraction
OT  - Solid phase extraction
OT  - UPLC-MS/MS
EDAT- 2019/08/28 06:00
MHDA- 2020/02/06 06:00
CRDT- 2019/08/28 06:00
PHST- 2019/04/04 00:00 [received]
PHST- 2019/06/23 00:00 [revised]
PHST- 2019/07/08 00:00 [accepted]
PHST- 2019/08/28 06:00 [entrez]
PHST- 2019/08/28 06:00 [pubmed]
PHST- 2020/02/06 06:00 [medline]
AID - S0039-9140(19)30770-2 [pii]
AID - 10.1016/j.talanta.2019.120144 [doi]
PST - ppublish
SO  - Talanta. 2019 Dec 1;205:120144. doi: 10.1016/j.talanta.2019.120144. Epub 2019 Jul 
      10.