PMID- 31451934
OWN - NLM
STAT- MEDLINE
DCOM- 20200601
LR  - 20200601
IS  - 1437-160X (Electronic)
IS  - 0172-8172 (Linking)
VI  - 39
IP  - 12
DP  - 2019 Dec
TI  - Association of biomarkers of inflammation and HLA-DRB1 gene locus with risk of 
      developing rheumatoid arthritis in females.
PG  - 2147-2157
LID - 10.1007/s00296-019-04429-y [doi]
AB  - Rheumatoid arthritis (RA) is an autoimmune disease causing chronic inflammation 
      of the joints. Multiple factors, including HLA-DRB1 gene variants, influence the 
      susceptibility to RA. The HLA-DRB1 gene is part of a family of genes called the 
      human leukocyte antigen (HLA) complex. In this study, we compared the 
      inflammatory biomarkers values, including erythrocyte sedimentation rate (ESR) 
      and C-reactive protein (CRP), between patients with RA and healthy control group 
      of females of the Public Institution Health Centre of Sarajevo Canton. In 
      addition, we estimated the frequencies of the HLA-DRB1 gene variants and their 
      association with the risk for RA development in females. The haematological and 
      biochemical tests were completed on automated analyzers. To assess the 
      association between the HLA-DRB genes and the risk of RA in females, 
      low-resolution genotyping of the HLA-DRB1, DRB3, DRB4, and DRB5 gene loci was 
      performed by the sequence-specific polymerase chain reaction method (PCR-SSP). 
      ESR and CRP were the most sensitive acute-phase reactants in females with RA and 
      there was a correlation between ESR and CRP values in RA patients. There was 
      significantly positive association between of the HLA-DRB1*03, *04, *08, *10, 
      *11, and *14 variants and elevated values of ESR in RA patients, but negative 
      between HLA-DRB1*03, *13 and *15 alleles and elevated CRP values. Furthermore, 
      our results confirm genetic susceptibility to RA in a female population to the 
      members of the HLA-DRB1*04 and *03 allelic groups, the DRB1*04/DRB1*04 and 
      DRB1*03/DRB1*04 genotypes, and the DRB1*04-DRB4* or DRB1*03-DRB3* haplotypes, 
      which, therefore, represent risk factors for the development of this disease. 
      According to our results, the DRB1*01/DRB1*15 and DRB1*07/DRB1*16 genotypes and 
      the HLA-DRB5 gene locus represent a protective factor for RA. The presence of 
      specific HLA-DRB1 gene variants increases the risk of developing RA, while other 
      variants provide protection against disease. Therefore, HLA typing could be 
      helpful in the prediction of RA development and establishing and confirming a 
      definitive diagnosis of autoimmune diseases in some subjects. A strong 
      association with the higher levels of ESR and CRP could be used to establish 
      definitive diagnosis and introduce of early treatment of RA to prevent the 
      occurrence of RA symptoms.
FAU - Klimenta, Biljana
AU  - Klimenta B
AUID- ORCID: 0000-0002-9788-6789
AD  - Department of Laboratory Diagnostics, The Public Institution Health Centre of 
      Sarajevo Canton, Sarajevo, Bosnia and Herzegovina.
FAU - Nefic, Hilada
AU  - Nefic H
AUID- ORCID: 0000-0002-7774-5743
AD  - Department of Biology, Faculty of Science, University of Sarajevo, Sarajevo, 
      Bosnia and Herzegovina. hnefic@gmail.com.
FAU - Prodanovic, Nenad
AU  - Prodanovic N
AUID- ORCID: 0000-0002-5534-9846
AD  - Faculty of Medicine, University of Banja Luka, Banja Luka, Bosnia and 
      Herzegovina.
FAU - Jadric, Radivoj
AU  - Jadric R
AUID- ORCID: 0000-0002-8197-5939
AD  - Department of Medical Biochemistry, Faculty of Medicine, University of Sarajevo, 
      Sarajevo, Bosnia and Herzegovina.
FAU - Hukic, Fatima
AU  - Hukic F
AUID- ORCID: 0000-0003-4700-911X
AD  - Department of Biochemistry, Clinical University Centre of Tuzla, Tuzla, Bosnia 
      and Herzegovina.
LA  - eng
PT  - Journal Article
DEP - 20190826
PL  - Germany
TA  - Rheumatol Int
JT  - Rheumatology international
JID - 8206885
RN  - 0 (Biomarkers)
RN  - 0 (HLA-DRB1 Chains)
SB  - IM
MH  - Adult
MH  - Alleles
MH  - Arthritis, Rheumatoid/blood/*genetics
MH  - Biomarkers/blood
MH  - Blood Sedimentation
MH  - Cross-Sectional Studies
MH  - Female
MH  - Gene Frequency
MH  - *Genetic Predisposition to Disease
MH  - *Genotype
MH  - HLA-DRB1 Chains/*genetics
MH  - Haplotypes
MH  - Humans
MH  - Inflammation/*blood/genetics
MH  - Middle Aged
MH  - Risk Factors
OTO - NOTNLM
OT  - C-reactive protein (CRP)
OT  - Erythrocyte sedimentation rate (ESR)
OT  - HLA-DRB1 gene
OT  - Rheumatoid arthritis (RA)
EDAT- 2019/08/28 06:00
MHDA- 2020/06/02 06:00
CRDT- 2019/08/28 06:00
PHST- 2019/07/13 00:00 [received]
PHST- 2019/08/19 00:00 [accepted]
PHST- 2019/08/28 06:00 [pubmed]
PHST- 2020/06/02 06:00 [medline]
PHST- 2019/08/28 06:00 [entrez]
AID - 10.1007/s00296-019-04429-y [pii]
AID - 10.1007/s00296-019-04429-y [doi]
PST - ppublish
SO  - Rheumatol Int. 2019 Dec;39(12):2147-2157. doi: 10.1007/s00296-019-04429-y. Epub 
      2019 Aug 26.