PMID- 31452562
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220410
IS  - 0353-8109 (Print)
IS  - 1986-5988 (Electronic)
IS  - 0353-8109 (Linking)
VI  - 27
IP  - 2
DP  - 2019 Jun
TI  - Phenotype Characteristics and Osteogenic Differentiation Potential of Human 
      Mesenchymal Stem Cells Derived from Amnion Membrane (HAMSCs) and Umbilical Cord 
      (HUC-MSCs).
PG  - 72-77
LID - 10.5455/aim.2019.27.72-77 [doi]
AB  - INTRODUCTION: Human amnion membrane mesenchymal stem cells (hAMSCs) and human 
      umbilical cord mesenchymal stem cells (hUC-MSCs) are potential, non invasive 
      sources of stem cells used for bone tissue engineering. Phenotyping 
      characterization is an extremely important consideration in the choice of the 
      appropriate passage in order to maximize its osteogenic differentiation 
      potential. AIM: To explore phenotype characteristics and compare osteogenic 
      differentiation potential of hAMSCs and hUC-MSCs. METHOD: Isolation and culture 
      were performed on hAMSCs and hUC-MSCs from a healthy woman in her 38(th) weeks of 
      pregnancy. CD90, CD105 and CD73 phenotype characterization was done in passage 
      4-7. An osteogenic differentiation examination of hAMSCs and hUC-MSCs with 
      Alizarin red staining and RUNX2 expression was performed in the passage that had 
      appropriate expressions of phenotype characteristics. RESULTS: The expression of 
      CD90 hUC-MSCs was higher than that of hAMSCs in all passages. CD105 hUC-MSCs was 
      higher in passage 4-6, while CD105 hAMSCs was equal to that of hUC-MSCs in 
      passage 7. CD73 hUC-MSCs was higher than hAMSCs in passage 4 and 5, while in 
      passage 6 and 7 hAMSCs was higher than hUC-MSCs. There was a decrease in the 
      number of CD90, CD105 and CD73 on hAMSCs and hUC-MSCs in passage 5, then 
      determined as appropriate passage. Alizarin red staining examination showed 
      calcium deposition and revealed no significant difference, but RUNX2 expression 
      of hUC-MSCs was significantly higher than that for hAMSCs. CONCLUSION: Both 
      hAMSCs and hUC-MSCs had phenotype characteristics of mesenchymal stem cell and 
      showed ostegenic differentiation potential.
FAU - Hendrijantini, Nike
AU  - Hendrijantini N
AD  - Department of Prosthodontic, Faculty of Dental Medicine, Universitas Airlangga, 
      Surabaya, Indonesia.
FAU - Hartono, Poedjo
AU  - Hartono P
AD  - Department of Obstetrics and Gynaecology, Dr. Soetomo General Hospital, Faculty 
      of Medicine, Universitas Airlangga, Surabaya, Indonesia.
LA  - eng
PT  - Journal Article
PL  - Bosnia and Herzegovina
TA  - Acta Inform Med
JT  - Acta informatica medica : AIM : journal of the Society for Medical Informatics of 
      Bosnia & Herzegovina : casopis Drustva za medicinsku informatiku BiH
JID - 101147064
PMC - PMC6688306
OTO - NOTNLM
OT  - Alizarin Red Immunohistochemistry
OT  - Mesenchymal Stem Cells
OT  - Osteogenesis
OT  - Phenotype Flow Cytometry
OT  - Umbilical Cord
COIS- There are no conflicts of interest.
EDAT- 2019/08/28 06:00
MHDA- 2019/08/28 06:01
PMCR- 2019/06/01
CRDT- 2019/08/28 06:00
PHST- 2019/08/28 06:00 [entrez]
PHST- 2019/08/28 06:00 [pubmed]
PHST- 2019/08/28 06:01 [medline]
PHST- 2019/06/01 00:00 [pmc-release]
AID - AIM-27-72 [pii]
AID - 10.5455/aim.2019.27.72-77 [doi]
PST - ppublish
SO  - Acta Inform Med. 2019 Jun;27(2):72-77. doi: 10.5455/aim.2019.27.72-77.