PMID- 31452653
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220410
IS  - 1654-661X (Print)
IS  - 1654-661X (Electronic)
IS  - 1654-661X (Linking)
VI  - 63
DP  - 2019
TI  - Aronia berry extract inhibits TNF-alpha-induced vascular endothelial inflammation 
      through the regulation of STAT3.
LID - 10.29219/fnr.v63.3361 [doi]
AB  - BACKGROUND: Inflammation in endothelial cells induces production of inflammatory 
      cytokines and monocytes adhesion, which are crucial events in the initiation of 
      atherosclerosis. Aronia berry (Aronia meranocalpa), also called black chokeberry, 
      contains abundant anthocyanins that have received considerable interest for their 
      possible relations to vascular health. OBJECTIVE: The aim of this study was to 
      investigate whether an anthocyanin-rich extract obtained from aronia berry can 
      attenuate inflammatory responses in vascular endothelial cells. METHODS: As a 
      model of vascular endothelial inflammation, human umbilical vein endothelial 
      cells (HUVECs) pretreated with aronia berry extract were stimulated with tumor 
      necrosis factor-alpha (TNF-alpha). The expression levels of cytokines and adhesion 
      molecules were analyzed. To investigate the effects of aronia berry extract on 
      the adhesion of THP-1 monocytic cell, the static adhesion assay was carried out. 
      The possible molecular mechanisms by which aronia berry extract regulated 
      vascular inflammatory responses were explored. RESULTS: The mRNA expressions of 
      interleukins (IL-1beta, IL-6, and IL-8) and monocyte chemoattractant protein-1 
      (MCP-1) upregulated by TNF-alpha were significantly suppressed by pretreatment with 
      aronia berry extract. Aronia berry extract decreased TNF-alpha-induced 
      monocyte/endothelial adhesion and suppressed vascular cell adhesion molecule-1 
      (VCAM-1) expression, but did not affect intercellular adhesion molecule-1 
      (ICAM-1) expression. Moreover, aronia berry extract decreased the phosphorylation 
      of signal transducer and activator of transcription 3 (STAT3) and the nuclear 
      levels of STAT3 and interferon regulatory transcription factor-1 (IRF1). The 
      nuclear translocation of nuclear factor-kappa B (NF-kappaB) was not inhibited by 
      aronia berry extract. CONCLUSION: Aronia berry extract could exert 
      anti-atherosclerotic effects on TNF-alpha-induced inflammation through inhibition of 
      STAT3/IRF1 pathway in vascular endothelial cells.
FAU - Iwashima, Tomomi
AU  - Iwashima T
AD  - Department of Food and Nutritional Sciences, Graduate School of Humanities and 
      Sciences, Ochanomizu University, Tokyo, Japan.
FAU - Kudome, Yuki
AU  - Kudome Y
AD  - Department of Food and Nutritional Sciences, Graduate School of Humanities and 
      Sciences, Ochanomizu University, Tokyo, Japan.
FAU - Kishimoto, Yoshimi
AU  - Kishimoto Y
AD  - Endowed Research Department "Food for Health," Ochanomizu University, Tokyo, 
      Japan.
FAU - Saita, Emi
AU  - Saita E
AD  - Endowed Research Department "Food for Health," Ochanomizu University, Tokyo, 
      Japan.
FAU - Tanaka, Miori
AU  - Tanaka M
AD  - Department of Food and Nutritional Sciences, Graduate School of Humanities and 
      Sciences, Ochanomizu University, Tokyo, Japan.
FAU - Taguchi, Chie
AU  - Taguchi C
AD  - Endowed Research Department "Food for Health," Ochanomizu University, Tokyo, 
      Japan.
FAU - Hirakawa, Satoshi
AU  - Hirakawa S
AD  - Pola Chemical Industries Inc., Kanagawa, Japan.
FAU - Mitani, Nobu
AU  - Mitani N
AD  - Pola Chemical Industries Inc., Kanagawa, Japan.
FAU - Kondo, Kazuo
AU  - Kondo K
AD  - Endowed Research Department "Food for Health," Ochanomizu University, Tokyo, 
      Japan.
AD  - Institute of Life Innovation Studies, Toyo University, Gunma, Japan.
FAU - Iida, Kaoruko
AU  - Iida K
AD  - Department of Food and Nutritional Sciences, Graduate School of Humanities and 
      Sciences, Ochanomizu University, Tokyo, Japan.
AD  - Institute for Human Life Innovation, Ochanomizu University, Tokyo, Japan.
LA  - eng
PT  - Journal Article
DEP - 20190816
PL  - Sweden
TA  - Food Nutr Res
JT  - Food & nutrition research
JID - 101488795
PMC - PMC6698673
OTO - NOTNLM
OT  - STAT3
OT  - aronia berry
OT  - atherosclerosis
OT  - inflammation
OT  - polyphenol
OT  - vascular endothelial cell
COIS- This work was partly supported by JSPS KAKENHI (Grant nos. 15H02895, 16H03033, 
      and 17K00847) from the Japan Society for the Promotion of Science. The authors 
      declare no potential conflicts of interest.
EDAT- 2019/08/28 06:00
MHDA- 2019/08/28 06:01
PMCR- 2019/08/16
CRDT- 2019/08/28 06:00
PHST- 2019/01/27 00:00 [received]
PHST- 2019/07/04 00:00 [revised]
PHST- 2019/07/15 00:00 [accepted]
PHST- 2019/08/28 06:00 [entrez]
PHST- 2019/08/28 06:00 [pubmed]
PHST- 2019/08/28 06:01 [medline]
PHST- 2019/08/16 00:00 [pmc-release]
AID - 3361 [pii]
AID - 10.29219/fnr.v63.3361 [doi]
PST - epublish
SO  - Food Nutr Res. 2019 Aug 16;63. doi: 10.29219/fnr.v63.3361. eCollection 2019.