PMID- 31452735
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231013
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 18
IP  - 3
DP  - 2019 Sep
TI  - HTR1D, TIMP1, SERPINE1, MMP3 and CNR2 affect the survival of patients with colon 
      adenocarcinoma.
PG  - 2448-2454
LID - 10.3892/ol.2019.10545 [doi]
AB  - Colorectal cancer (CRC) is a tumor that derives from the rectum or colon, and 
      colon adenocarcinoma (COAD) is the most common type of CRC. The present study was 
      performed to identify genes that serve critical roles in the survival of patients 
      with COAD. RNA-sequencing data of COAD was extracted from The Cancer Genome Atlas 
      database, which included 480 tumor samples and 41 normal samples. Using the limma 
      package, differential expression analysis was performed to identify the 
      differentially expressed genes (DEGs). In addition, the potential functions and 
      pathways for the identified DEGs were analyzed using the clusterProfiler package. 
      After the samples were divided into high and low expression groups, survival 
      analysis for the two groups was performed using the Kaplan-Meier model. Using 
      Cytoscape software, a protein-protein interaction network was generated for the 
      survival-associated genes. A total of 1,519 DEGs, including 568 upregulated genes 
      and 951 downregulated genes, were identified in the COAD samples. Enrichment 
      analysis suggested that the DEGs were implicated in numerous functional terms and 
      pathways. Furthermore, 109 DEGs were identified to be survival-associated genes 
      in COAD. According to the degrees of the network nodes, 5-hydroxytryptamine 
      receptor 1D (HTR1D), TIMP metallopeptidase inhibitor 1 (TIMP1), serpin family E 
      member 1 (SERPINE1), matrix metallopeptidase 3 (MMP3) and cannabinoid receptor 2 
      (CNR2) were key nodes, and the expression levels of these genes were analyzed in 
      clinical samples of CRC. Therefore, the results of the present study suggest 
      HTR1D, TIMP1, SERPINE1, MMP3 and CNR2 may affect the prognosis of patients with 
      COAD.
FAU - Zeng, Chunyan
AU  - Zeng C
AD  - Department of Gastroenterology, The First Affiliated Hospital of Nanchang 
      University, Nanchang, Jiangxi 330006, P.R. China.
FAU - Chen, Youxiang
AU  - Chen Y
AD  - Department of Gastroenterology, The First Affiliated Hospital of Nanchang 
      University, Nanchang, Jiangxi 330006, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190628
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC6676656
OTO - NOTNLM
OT  - colon adenocarcinoma
OT  - differentially expressed genes
OT  - enrichment analysis
OT  - protein-protein interaction network
OT  - survival analysis
EDAT- 2019/08/28 06:00
MHDA- 2019/08/28 06:01
PMCR- 2019/06/28
CRDT- 2019/08/28 06:00
PHST- 2018/08/09 00:00 [received]
PHST- 2019/05/09 00:00 [accepted]
PHST- 2019/08/28 06:00 [entrez]
PHST- 2019/08/28 06:00 [pubmed]
PHST- 2019/08/28 06:01 [medline]
PHST- 2019/06/28 00:00 [pmc-release]
AID - OL-0-0-10545 [pii]
AID - 10.3892/ol.2019.10545 [doi]
PST - ppublish
SO  - Oncol Lett. 2019 Sep;18(3):2448-2454. doi: 10.3892/ol.2019.10545. Epub 2019 Jun 
      28.