PMID- 31454398 OWN - NLM STAT- MEDLINE DCOM- 20200303 LR - 20200303 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 14 IP - 8 DP - 2019 TI - Effect of daptomycin and vancomycin on Staphylococcus epidermidis biofilms: An in vitro assessment using fluorescence in situ hybridization. PG - e0221786 LID - 10.1371/journal.pone.0221786 [doi] LID - e0221786 AB - Colonization of in-dwelling catheters by microbial biofilms is a major concern in patient health eventually leading to catheter-related blood stream infections. Biofilms are less susceptible to standard antibiotic therapies that are effective against planktonic bacteria. Standard procedure for the detection of microorganisms on the catheter tip is culture. However, viable but non-culturable cells (VBNCs) may be missed. The aim of this study was to evaluate the use of fluorescence in situ hybridization (FISH) as an indicator to visualize and quantify the effect of the antibiotics daptomycin and vancomycin on biofilms in situ. We established an in vitro catheter biofilm model of Staphylococcus epidermidis biofilms on polyurethane catheters. Biofilm activity was measured by FISH and correlated to colony forming units (CFU) data. Digital image analysis was used for quantification of total biofilm mass and the area of the FISH positive biofilm cells. FISH showed a pronounced effect of both antibiotics on the biofilms, with daptomycin having a significantly stronger effect in terms of both reduction of biofilm mass and number of FISH-positive cells. This supports the anti-biofilm capacity of daptomycin. Interestingly, neither antibiotic was able to eradicate all of the FISH-positive cells. In summary, FISH succeeded in visualization, quantification, and localization of antibiotic activity on biofilms. This technique adds a new tool to the arsenal of test systems for anti-biofilm compounds. FISH is a valuable complementary technique to CFU since it can be highly standardized and provides information on biofilm architecture and quantity and localization of survivor cells. FAU - Sutrave, S AU - Sutrave S AUID- ORCID: 0000-0002-2040-1659 AD - Biofilmcenter, Institute for Microbiology, Infectious Diseases and Immunology, Charite - Universitatsmedizin Berlin, corporate member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Kikhney, J AU - Kikhney J AUID- ORCID: 0000-0002-4849-7947 AD - Biofilmcenter, Institute for Microbiology, Infectious Diseases and Immunology, Charite - Universitatsmedizin Berlin, corporate member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Schmidt, J AU - Schmidt J AD - Biofilmcenter, Institute for Microbiology, Infectious Diseases and Immunology, Charite - Universitatsmedizin Berlin, corporate member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Petrich, A AU - Petrich A AD - Biofilmcenter, Institute for Microbiology, Infectious Diseases and Immunology, Charite - Universitatsmedizin Berlin, corporate member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Wiessner, A AU - Wiessner A AD - Biofilmcenter, Institute for Microbiology, Infectious Diseases and Immunology, Charite - Universitatsmedizin Berlin, corporate member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Kursawe, Laura AU - Kursawe L AD - Biofilmcenter, Institute for Microbiology, Infectious Diseases and Immunology, Charite - Universitatsmedizin Berlin, corporate member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Gebhardt, M AU - Gebhardt M AD - Biofilmcenter, Institute for Microbiology, Infectious Diseases and Immunology, Charite - Universitatsmedizin Berlin, corporate member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Kertzscher, U AU - Kertzscher U AD - Biofluid Mechanics Laboratory, Charite - Universitatsmedizin Berlin, corporate member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Gabel, G AU - Gabel G AD - Biofluid Mechanics Laboratory, Charite - Universitatsmedizin Berlin, corporate member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Goubergrits, L AU - Goubergrits L AD - Biofluid Mechanics Laboratory, Charite - Universitatsmedizin Berlin, corporate member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Affeld, K AU - Affeld K AD - Biofluid Mechanics Laboratory, Charite - Universitatsmedizin Berlin, corporate member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Moter, A AU - Moter A AD - Biofilmcenter, Institute for Microbiology, Infectious Diseases and Immunology, Charite - Universitatsmedizin Berlin, corporate member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. LA - eng PT - Journal Article DEP - 20190827 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (Anti-Bacterial Agents) RN - 6Q205EH1VU (Vancomycin) RN - NWQ5N31VKK (Daptomycin) SB - IM MH - Anti-Bacterial Agents/pharmacology MH - Biofilms/*drug effects MH - Bioreactors/microbiology MH - Catheters, Indwelling/microbiology MH - Colony Count, Microbial MH - Daptomycin/*pharmacology MH - Image Processing, Computer-Assisted MH - *In Situ Hybridization, Fluorescence MH - Staphylococcus epidermidis/*drug effects/growth & development/*physiology MH - Vancomycin/*pharmacology PMC - PMC6711592 COIS- The authors have declared that no competing interests exist. EDAT- 2019/08/28 06:00 MHDA- 2020/03/04 06:00 PMCR- 2019/08/27 CRDT- 2019/08/28 06:00 PHST- 2019/05/09 00:00 [received] PHST- 2019/08/14 00:00 [accepted] PHST- 2019/08/28 06:00 [entrez] PHST- 2019/08/28 06:00 [pubmed] PHST- 2020/03/04 06:00 [medline] PHST- 2019/08/27 00:00 [pmc-release] AID - PONE-D-19-13199 [pii] AID - 10.1371/journal.pone.0221786 [doi] PST - epublish SO - PLoS One. 2019 Aug 27;14(8):e0221786. doi: 10.1371/journal.pone.0221786. eCollection 2019.