PMID- 31460824
OWN - NLM
STAT- MEDLINE
DCOM- 20210701
LR  - 20210701
IS  - 1557-7465 (Electronic)
IS  - 1079-9907 (Linking)
VI  - 40
IP  - 1
DP  - 2020 Jan
TI  - Single Triglyceride-Rich Meal Destabilizes Barrier Functions and Initiates 
      Inflammatory Processes of Endothelial Cells.
PG  - 43-53
LID - 10.1089/jir.2018.0173 [doi]
AB  - Postprandial hypertriglyceridemia is an independent risk factor for 
      cardiovascular disease. The aim of this study was to assess the effects of a 
      single fat-rich meal on barrier functions and inflammatory status on human 
      umbilical vascular endothelial cells (HUVECs), furthermore we assess the effects 
      of mixture of palmitic acid and 25-hydroxycholesterol (PA +25OHCH) on integrity 
      of endothelial cells and their inflammatory properties. HUVECs were induced with 
      serum of healthy volunteers taken before, and 3 h after, the consumption of a 
      meal with a standardized daily required dose of fats. In addition, endothelial 
      cells were induced with PA +25OHCH (800 muM/L+10 mug/mL). Total cholesterol, 
      triglycerides (TGs), high-density lipoprotein cholesterol, low-density 
      lipoprotein cholesterol, high sensitivity c-reactive protein, and glucose were 
      measured at fasting and postprandially. HUVEC integrity was measured in the 
      RTCA-DP xCELLigence system. mRNA expression of interleukin (IL)-33, IL-32, 
      intercellular adhesion molecule-1 (ICAM-1), monocyte chemoattractant protein-1 
      (MCP-1), CX3C-chemokine, vascular endothelial growth factor (VEGF) occludin, and 
      VE-cadherin was analyzed by real-time polymerase chain reaction. Viability and 
      apoptosis were assessed in flow cytometry. The level of VEGF and IL-33 in fasting 
      and postprandial serum was assessed by enzyme-linked immunosorbent assay (ELISA). 
      Three hours after consumption of a fatty meal, all patients displayed increased 
      levels of TGs and Toll-like receptors (110 +/- 37 mg/dL versus 182 +/- 64 mg/dL 
      P < 0.05) (24 +/- 11 mg/dL versus 42 +/- 14 mg/dL P < 0.05). Postprandial serum and 
      PA +25OHCH caused >20% decrease of HUVEC integrity than fasting serum 
      (P < 0.001). HUVEC disintegration was accompanied by a decrease of occludin mRNA 
      expression as compared with fasting serum (P < 0.05). The fatty meal affected 
      neither VE-cadherin mRNA expression nor its apoptosis (P > 0.05). Mixture of PA 
      +25OHCH caused decrease of VE-cadherin mRNA expression as compared with fasting 
      serum (P < 0.01). PA +25OHCH did not affect HUVEC apoptosis (P > 0.05). 
      Postprandial serum and PA +25OHCH caused increase of IL-33, MCP-1, ICAM-1, IL-32, 
      VEGF, and CX3C-chemokine mRNA expression as compared with fasting serum 
      (P < 0.05). Moreover, level of VEGF in fatty serum was significantly higher 
      (P < 0.001). Postprandial lipemia after a single fatty meal may destabilize the 
      endothelial barrier and initiate inflammatory processes.
FAU - Gorzelak-Pabis, Paulina
AU  - Gorzelak-Pabis P
AD  - The Laboratory of Tissue Immunopharmacology, Department of Internal Diseases and 
      Clinical Pharmacology, Medical University of Lodz, Lodz, Poland.
FAU - Wozniak, Ewelina
AU  - Wozniak E
AD  - The Laboratory of Tissue Immunopharmacology, Department of Internal Diseases and 
      Clinical Pharmacology, Medical University of Lodz, Lodz, Poland.
FAU - Wojdan, Katarzyna
AU  - Wojdan K
AD  - The Laboratory of Tissue Immunopharmacology, Department of Internal Diseases and 
      Clinical Pharmacology, Medical University of Lodz, Lodz, Poland.
FAU - Chalubinski, Maciej
AU  - Chalubinski M
AD  - The Laboratory of Tissue Immunopharmacology, Department of Internal Diseases and 
      Clinical Pharmacology, Medical University of Lodz, Lodz, Poland.
FAU - Broncel, Marlena
AU  - Broncel M
AD  - The Laboratory of Tissue Immunopharmacology, Department of Internal Diseases and 
      Clinical Pharmacology, Medical University of Lodz, Lodz, Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190828
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (Cytokines)
RN  - 0 (Hydroxycholesterols)
RN  - 0 (RNA, Messenger)
RN  - 0 (Triglycerides)
RN  - 2V16EO95H1 (Palmitic Acid)
RN  - 767JTD2N31 (25-hydroxycholesterol)
SB  - IM
MH  - Adult
MH  - Cells, Cultured
MH  - Cytokines/genetics/metabolism
MH  - Female
MH  - Human Umbilical Vein Endothelial Cells/*drug effects/metabolism
MH  - Humans
MH  - Hydroxycholesterols/pharmacology
MH  - Inflammation/*chemically induced/metabolism
MH  - Male
MH  - Meals
MH  - Palmitic Acid/pharmacology
MH  - Postprandial Period
MH  - RNA, Messenger/genetics/metabolism
MH  - Triglycerides/*administration & dosage/*adverse effects/blood
OTO - NOTNLM
OT  - ICAM-1
OT  - IL-32
OT  - IL-33
OT  - VEGF
OT  - endothelial cells
OT  - postprandial hypertriglyceridemia
EDAT- 2019/08/29 06:00
MHDA- 2021/07/02 06:00
CRDT- 2019/08/29 06:00
PHST- 2019/08/29 06:00 [pubmed]
PHST- 2021/07/02 06:00 [medline]
PHST- 2019/08/29 06:00 [entrez]
AID - 10.1089/jir.2018.0173 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2020 Jan;40(1):43-53. doi: 10.1089/jir.2018.0173. Epub 
      2019 Aug 28.