PMID- 31463716
OWN - NLM
STAT- MEDLINE
DCOM- 20191108
LR  - 20191108
IS  - 1432-1335 (Electronic)
IS  - 0171-5216 (Linking)
VI  - 145
IP  - 11
DP  - 2019 Nov
TI  - Heterogeneity of childhood acute leukemia with mature B-cell immunophenotype.
PG  - 2803-2811
LID - 10.1007/s00432-019-03010-1 [doi]
AB  - BACKGROUND: Flow cytometry (FCM) plays a crucial role in the differential 
      diagnosis of Burkitt lymphoma/leukemia (BL) and B-cell precursor acute 
      lymphoblastic leukemia (BCP-ALL). The presence of surface IgM (sIgM) alone or 
      with light chain restriction indicates a mature blast phenotype (BIV by EGIL) and 
      is usually observed in BL. However, sIgM expression could also be detected in 
      transitional BCP-ALL cases. These similarities in immunophenotype and ambiguous 
      correspondence with other laboratory findings may challenge the correct BL 
      diagnostics. METHODS: We retrospectively reviewed the available data from 
      immunophenotypic, morphological, cytogenetic, and molecular genetic studies of 
      146 children (85 boys and 61 girls) with a median age of 10 years (range 
      0-18 years) who were diagnosed with BL and BCP-ALL. The blasts' immunophenotype 
      was studied by multicolor FCM. The conventional cytogenetic analysis included 
      G-banded karyotyping and fluorescence in situ hybridization (FISH). RESULTS: In 
      54 children classified as BIV-ALL according to the EGIL, it was demonstrated that 
      sIgM in a minority of cases can be associated with various types of BCP-ALL. 
      Analysis of the antigen expression profile of 105 patients with verified BL 
      (n = 21) and BCP-ALL (n = 84) showed significant differences in BL and the 
      sIgM(+) vs BCP-ALL immunophenotype. Thus, even in cases of ambiguous sIgM 
      expression, these two diseases could be reliably discriminated by complex 
      immunophenotyping. Moreover, 10 patients (7 boys and 3 girls) with BL leukemic 
      cells did not express sIgM, and they were diagnosed with BL on the basis of other 
      laboratory and clinical signs. CONCLUSIONS: In conclusion, our study shows that 
      BIV subtype is heterogeneous group of leukemia including not only the BL, but 
      also BCP-ALL. In ambiguous cases, only a combination of multiple 
      immunophenotypic, cytomorphologic, and genetic diagnostic technologies can allow 
      the precise discrimination of BL and BCP-ALL and selection of the appropriate 
      treatment scheme.
FAU - Demina, Irina
AU  - Demina I
AD  - Dmitry Rogachev National Medical Research Center of Pediatric Hematology, 
      Oncology and Immunology, 1 Samory Mashela St., GSP-7, Moscow, 117997, Russia.
FAU - Zerkalenkova, Elena
AU  - Zerkalenkova E
AD  - Dmitry Rogachev National Medical Research Center of Pediatric Hematology, 
      Oncology and Immunology, 1 Samory Mashela St., GSP-7, Moscow, 117997, Russia.
FAU - Illarionova, Olga
AU  - Illarionova O
AD  - Dmitry Rogachev National Medical Research Center of Pediatric Hematology, 
      Oncology and Immunology, 1 Samory Mashela St., GSP-7, Moscow, 117997, Russia.
FAU - Olshanskaya, Yulia
AU  - Olshanskaya Y
AD  - Dmitry Rogachev National Medical Research Center of Pediatric Hematology, 
      Oncology and Immunology, 1 Samory Mashela St., GSP-7, Moscow, 117997, Russia.
FAU - Verzhbitskaya, Tatiana
AU  - Verzhbitskaya T
AD  - Sverdlovsk Regional Clinical Children's Hospital No 1, 32 Serafimy Deryabinoy 
      St., Ekaterinburg, 620149, Russia.
AD  - Research Institute of Medical Cell Technologies, 22a Karla Marksa St., 
      Ekaterinburg, 620026, Russia.
FAU - Semchenkova, Alexandra
AU  - Semchenkova A
AD  - Dmitry Rogachev National Medical Research Center of Pediatric Hematology, 
      Oncology and Immunology, 1 Samory Mashela St., GSP-7, Moscow, 117997, Russia.
FAU - Tsaur, Grigory
AU  - Tsaur G
AD  - Sverdlovsk Regional Clinical Children's Hospital No 1, 32 Serafimy Deryabinoy 
      St., Ekaterinburg, 620149, Russia.
AD  - Research Institute of Medical Cell Technologies, 22a Karla Marksa St., 
      Ekaterinburg, 620026, Russia.
AD  - Federal State Autonomous Educational Institution of Higher Education Ural Federal 
      University named after the first President of Russia B.N.Yeltsin, 19 Mira Street, 
      Ekaterinburg, 620002, Russia.
FAU - Rusanova, Ekaterina
AU  - Rusanova E
AD  - I.P. Pavlov First Saint Petersburg State Medical University, 6-8 L'va Tolstogo 
      St., Saint Petersburg, 197022, Russia.
FAU - Belogurova, Margarita
AU  - Belogurova M
AD  - St. Petersburg Clinical Scientific and Practical Center of Specialized Medical 
      Assistance (Oncological), 68a Leningradskaya St., Pesochnyi, Saint Petersburg, 
      197758, Russia.
FAU - Baidun, Ludmila
AU  - Baidun L
AD  - Russian Children Clinical Hospital, Ministry of Health of Russia, 117 Leninskiy 
      Prospect, Moscow, 119571, Russia.
FAU - Plyasunova, Svetlana
AU  - Plyasunova S
AD  - Dmitry Rogachev National Medical Research Center of Pediatric Hematology, 
      Oncology and Immunology, 1 Samory Mashela St., GSP-7, Moscow, 117997, Russia.
FAU - Konyuhova, Tatiana
AU  - Konyuhova T
AD  - Dmitry Rogachev National Medical Research Center of Pediatric Hematology, 
      Oncology and Immunology, 1 Samory Mashela St., GSP-7, Moscow, 117997, Russia.
FAU - Kazakova, Anna
AU  - Kazakova A
AD  - Dmitry Rogachev National Medical Research Center of Pediatric Hematology, 
      Oncology and Immunology, 1 Samory Mashela St., GSP-7, Moscow, 117997, Russia.
FAU - Fechina, Larisa
AU  - Fechina L
AD  - Sverdlovsk Regional Clinical Children's Hospital No 1, 32 Serafimy Deryabinoy 
      St., Ekaterinburg, 620149, Russia.
AD  - Research Institute of Medical Cell Technologies, 22a Karla Marksa St., 
      Ekaterinburg, 620026, Russia.
FAU - Novichkova, Galina
AU  - Novichkova G
AD  - Dmitry Rogachev National Medical Research Center of Pediatric Hematology, 
      Oncology and Immunology, 1 Samory Mashela St., GSP-7, Moscow, 117997, Russia.
FAU - Samochatova, Elena
AU  - Samochatova E
AD  - Dmitry Rogachev National Medical Research Center of Pediatric Hematology, 
      Oncology and Immunology, 1 Samory Mashela St., GSP-7, Moscow, 117997, Russia.
FAU - Myakova, Natalia
AU  - Myakova N
AD  - Dmitry Rogachev National Medical Research Center of Pediatric Hematology, 
      Oncology and Immunology, 1 Samory Mashela St., GSP-7, Moscow, 117997, Russia.
FAU - Maschan, Alexey
AU  - Maschan A
AD  - Dmitry Rogachev National Medical Research Center of Pediatric Hematology, 
      Oncology and Immunology, 1 Samory Mashela St., GSP-7, Moscow, 117997, Russia.
FAU - Popov, Alexander M
AU  - Popov AM
AUID- ORCID: 0000-0002-0889-6986
AD  - Dmitry Rogachev National Medical Research Center of Pediatric Hematology, 
      Oncology and Immunology, 1 Samory Mashela St., GSP-7, Moscow, 117997, Russia. 
      uralcytometry@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20190828
PL  - Germany
TA  - J Cancer Res Clin Oncol
JT  - Journal of cancer research and clinical oncology
JID - 7902060
SB  - IM
MH  - Adolescent
MH  - B-Lymphocytes/*pathology
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Flow Cytometry/*methods
MH  - Follow-Up Studies
MH  - Humans
MH  - Immunophenotyping/*methods
MH  - Infant
MH  - Infant, Newborn
MH  - Karyotyping/*methods
MH  - Male
MH  - Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/*classification/*pathology
MH  - Prognosis
MH  - Retrospective Studies
OTO - NOTNLM
OT  - BCP-ALL
OT  - Burkitt lymphoma
OT  - Differential diagnostics
OT  - Flow cytometry
EDAT- 2019/08/30 06:00
MHDA- 2019/11/09 06:00
CRDT- 2019/08/30 06:00
PHST- 2019/07/03 00:00 [received]
PHST- 2019/08/19 00:00 [accepted]
PHST- 2019/08/30 06:00 [pubmed]
PHST- 2019/11/09 06:00 [medline]
PHST- 2019/08/30 06:00 [entrez]
AID - 10.1007/s00432-019-03010-1 [pii]
AID - 10.1007/s00432-019-03010-1 [doi]
PST - ppublish
SO  - J Cancer Res Clin Oncol. 2019 Nov;145(11):2803-2811. doi: 
      10.1007/s00432-019-03010-1. Epub 2019 Aug 28.