PMID- 31464075 OWN - NLM STAT- MEDLINE DCOM- 20200518 LR - 20200518 IS - 1930-739X (Electronic) IS - 1930-7381 (Linking) VI - 27 IP - 10 DP - 2019 Oct TI - Microarray Profiling and Coexpression Network Analysis of Long Noncoding RNAs in Adipose Tissue of Obesity-T2DM Mouse. PG - 1644-1651 LID - 10.1002/oby.22590 [doi] AB - OBJECTIVE: The aim of this study was to understand more about long noncoding RNAs (lncRNAs) as potential prediction biomarkers or therapeutic targets for obesity and type 2 diabetes mellitus (T2DM). This study aimed to find more lncRNA candidates related to obesity and T2DM. METHODS: In this study, a high-fat diet (HFD)-induced obesity-T2DM mouse model was used, and a mRNA and lncRNA expression map was drawn up in adipose tissue by microarray technology. Then Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis were performed and revealed that the most associated genes and pathways were metabolism-related ones. The candidate lncRNA expression was further validated in adipose tissue from HFD-induced mice by quantitative real-time polymerase chain reaction analysis. RESULTS: Transcriptome analyses were performed to show expression profiles of mRNAs and lncRNAs in epididymal adipose tissue in the obesity-T2DM mice. A total of 124 lncRNAs and 1,606 mRNAs were differentially expressed between the chow and HFD groups. Then, an mRNA-lncRNA coexpression network was constructed. Based on a series of analyses, 15 candidate lncRNAs were screened, and their expression was further validated by quantitative real-time polymerase chain reaction analysis. CONCLUSIONS: The results reveal significant differences between the transcriptomes of the HFD and control groups in adipose tissue that provide clues to the molecular mechanisms of diet-induced metabolic disorders as well as biomarkers of risk for these disorders. CI - (c) 2019 The Obesity Society. FAU - Yao, Zhenyu AU - Yao Z AUID- ORCID: 0000-0002-5796-7189 AD - Center for Translational Medicine Research and Development, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China. FAU - Liu, Chang AU - Liu C AD - Research Center for Reproduction and Health Development, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China. AD - Shenzhen College of Advanced Technology, University of Chinese Academy of Sciences, Shenzhen, Guangdong, China. FAU - Yu, Xiangfang AU - Yu X AD - Research Center for Reproduction and Health Development, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China. AD - Shenzhen College of Advanced Technology, University of Chinese Academy of Sciences, Shenzhen, Guangdong, China. FAU - Meng, Jun AU - Meng J AD - Department of Microbiology, Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong, China. FAU - Teng, Bin AU - Teng B AD - Research Center for Reproduction and Health Development, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China. AD - Shenzhen College of Advanced Technology, University of Chinese Academy of Sciences, Shenzhen, Guangdong, China. FAU - Sun, Yutao AU - Sun Y AD - Center for Translational Medicine Research and Development, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China. FAU - Kerem, Goher AU - Kerem G AD - Research Center for Reproduction and Health Development, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China. AD - Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Urumqi, China. FAU - Ismayil, Aynur AU - Ismayil A AD - Research Center for Reproduction and Health Development, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China. AD - Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Urumqi, China. FAU - Fang, Peng AU - Fang P AD - CAS Key Laboratory of Human-Machine Intelligence-Synergy Systems, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China. FAU - Zhang, Jian V AU - Zhang JV AD - Research Center for Reproduction and Health Development, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China. AD - Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China. FAU - Ren, Pei-Gen AU - Ren PG AD - Research Center for Reproduction and Health Development, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190829 PL - United States TA - Obesity (Silver Spring) JT - Obesity (Silver Spring, Md.) JID - 101264860 RN - 0 (RNA, Long Noncoding) SB - IM MH - Adipose Tissue/*metabolism MH - Animals MH - Diabetes Mellitus, Type 2/complications/*genetics/pathology MH - Diet, High-Fat MH - Gene Expression Profiling MH - Gene Regulatory Networks/*genetics MH - Male MH - Mice MH - Mice, Inbred C57BL MH - Microarray Analysis MH - Obesity/complications/*genetics/pathology MH - RNA, Long Noncoding/*genetics/metabolism MH - Real-Time Polymerase Chain Reaction MH - Transcriptome EDAT- 2019/08/30 06:00 MHDA- 2020/05/19 06:00 CRDT- 2019/08/30 06:00 PHST- 2019/05/02 00:00 [received] PHST- 2019/06/16 00:00 [accepted] PHST- 2019/08/30 06:00 [pubmed] PHST- 2020/05/19 06:00 [medline] PHST- 2019/08/30 06:00 [entrez] AID - 10.1002/oby.22590 [doi] PST - ppublish SO - Obesity (Silver Spring). 2019 Oct;27(10):1644-1651. doi: 10.1002/oby.22590. Epub 2019 Aug 29.