PMID- 31464358
OWN - NLM
STAT- MEDLINE
DCOM- 20200127
LR  - 20200127
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 127
IP  - 6
DP  - 2019 Dec
TI  - A phenotypic screening bioassay for Escherichia coli stress and antibiotic 
      responses based on Fourier-transform infrared (FTIR) spectroscopy and 
      multivariate analysis.
PG  - 1776-1789
LID - 10.1111/jam.14429 [doi]
AB  - AIMS: To develop and optimize a Fourier-transform infrared spectroscopy (FTIRS) 
      phenotypic screening bioassay for stress responses, regarding the effect of 
      nutrient content, bacterial growth phase and stress agent exposure time. METHODS 
      AND RESULTS: A high-throughput FTIRS bioassay was developed to distinguish the 
      stress responses of Escherichia coli to sodium hydroxide, hydrochloric acid, 
      sodium chloride, sodium hypochlorite and ethanol. Principal component analysis 
      and hierarchical clustering were used to quantify the effect of each parameter on 
      bioassay performance, namely its reproducibility and metabolic resolution. 
      Bioassay performance varied greatly, ranging from poor to very good. Spectra were 
      partitioned into biologically relevant regions to evaluate their contributions to 
      bioassay performance, but further improvements were not observed. Bioassay 
      optimization was validated against empirical parameters, which confirmed a closer 
      representation of known mechanisms on the antibiotic-induced stress responses. 
      CONCLUSIONS: The optimized bioassay used standard nutrient content, cells in the 
      late-stationary growth phase and a one-shift exposure duration. Only the 
      optimized bioassay adequately and reproducibly distinguished the E. coli stress 
      and antibiotic responses. The absence of performance improvements using 
      partitioned spectra indicated that stress responses are imprinted on the 
      whole-spectra metabolic signature. SIGNIFICANCE AND IMPACT OF THE STUDY: Highly 
      optimized FTIRS bioassay parameters are vital in capturing whole-spectra 
      metabolic signatures that can be used for satisfactory and reproducible 
      phenotypic screening of stress and antibiotic responses.
CI  - (c) 2019 The Society for Applied Microbiology.
FAU - Ribeiro da Cunha, B
AU  - Ribeiro da Cunha B
AUID- ORCID: 0000-0002-0303-9416
AD  - iBB - Institute of Bioengineering and Biosciences (iBB), Instituto Superior 
      Tecnico (IST), Universidade de Lisboa, Lisboa, Portugal.
AD  - ISEL - Instituto Superior de Engenharia de Lisboa (ISEL), Instituto Politecnico 
      de Lisboa (IPL), Lisboa, Portugal.
FAU - Fonseca, L P
AU  - Fonseca LP
AD  - iBB - Institute of Bioengineering and Biosciences (iBB), Instituto Superior 
      Tecnico (IST), Universidade de Lisboa, Lisboa, Portugal.
FAU - Calado, C R C
AU  - Calado CRC
AD  - ISEL - Instituto Superior de Engenharia de Lisboa (ISEL), Instituto Politecnico 
      de Lisboa (IPL), Lisboa, Portugal.
LA  - eng
GR  - IDI&CA/IPL/2017/DrugsPlatf/ISEL/Instituto Politecnico de Lisboa/
GR  - IDI&CA/IPL/2018/RenalProg/ISEL/Instituto Politecnico de Lisboa/
PT  - Journal Article
DEP - 20191008
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Bacteriological Techniques/*methods
MH  - Escherichia coli/*drug effects
MH  - High-Throughput Screening Assays
MH  - *Multivariate Analysis
MH  - Phenotype
MH  - Reproducibility of Results
MH  - *Spectroscopy, Fourier Transform Infrared
MH  - Stress, Physiological/*drug effects
OTO - NOTNLM
OT  - Escherichia coli
OT  - Fourier-transform infrared spectroscopy
OT  - antibiotics
OT  - metabolism
OT  - optimization
OT  - stress response
EDAT- 2019/08/30 06:00
MHDA- 2020/01/28 06:00
CRDT- 2019/08/30 06:00
PHST- 2019/02/14 00:00 [received]
PHST- 2019/08/21 00:00 [revised]
PHST- 2019/08/21 00:00 [accepted]
PHST- 2019/08/30 06:00 [pubmed]
PHST- 2020/01/28 06:00 [medline]
PHST- 2019/08/30 06:00 [entrez]
AID - 10.1111/jam.14429 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2019 Dec;127(6):1776-1789. doi: 10.1111/jam.14429. Epub 2019 
      Oct 8.