PMID- 31465717
OWN - NLM
STAT- MEDLINE
DCOM- 20201218
LR  - 20230710
IS  - 1840-4812 (Electronic)
IS  - 1512-8601 (Print)
IS  - 1512-8601 (Linking)
VI  - 20
IP  - 1
DP  - 2020 Feb 5
TI  - Tissue-based metabolomics reveals potential biomarkers for cervical carcinoma and 
      HPV infection.
PG  - 78-87
LID - 10.17305/bjbms.2019.4359 [doi]
AB  - Aberrant metabolic regulation has been observed in human cancers, but the 
      corresponding regulation in human papillomavirus (HPV) infection-associated 
      cervical cancer is not well understood. Here, we explored potential biomarkers 
      for the early prediction of cervical carcinoma based on the metabolic profile of 
      uterine cervical tissue specimens that were positive for HPV16 infection. 
      Fifty-two fresh cervical tissues were collected from women confirmed to have 
      cervical squamous cell carcinoma (SCC; n = 21) or cervical intraepithelial 
      neoplasia (CIN) stages II-III (n = 20). Eleven healthy women constituted the 
      controls (negative controls [NCs]). Real-time polymerase chain reaction (PCR) was 
      performed to detect HPV infection in the tissues. High-resolution magic angle 
      spinning nuclear magnetic resonance was utilized for the analysis of the 
      metabolic profile in the tissues. The expression of rate-limiting enzymes 
      involved in key metabolic pathways was detected by reverse-transcription 
      quantitative PCR. An independent immunohistochemical analysis was performed using 
      123 cases of paraffin-embedded cervical specimens. A profile of 17 small 
      molecular metabolites that showed differential expression in HPV16-positive 
      cervical SCC or CIN II-III compared with HPV-negative NC group was identified. 
      According to the profile, the levels of alpha- and beta-glucose decreased, those of 
      lactate and low-density lipoproteins increased, and the expression of multiple 
      amino acids was altered. Significantly increased transcript and protein levels of 
      glycogen synthase kinase 3 beta (GSK3beta) and glutamate decarboxylase 1 (GAD1) and 
      decreased transcript and protein levels of pyruvate kinase muscle isozyme 2 
      (PKM2) and carnitine palmitoyltransferase 1A (CPT1A) were observed in the patient 
      group (p < 0.05). HPV infection and cervical carcinogenesis drive metabolic 
      modifications that might be associated with the aberrant regulation of enzymes 
      related to metabolic pathways.
FAU - Abudula, Abulizi
AU  - Abudula A
AD  - Department of Labour and Environmental Hygienics, School of Public Health, 
      Xinjiang Medical University, Urumqi, China. aabulizi@163.com.
FAU - Rouzi, Nuermanguli
AU  - Rouzi N
AD  - Department of Labour and Environmental Hygienics, School of Public Health, 
      Xinjiang Medical University, Urumqi, China. rnurimangul@163.com.
FAU - Xu, Lixiu
AU  - Xu L
AD  - Department of Pathology, School of Basic Medicine, Xinjiang Medical University, 
      Urumqi, China. 15299128270@163.com.
FAU - Yang, Yun
AU  - Yang Y
AD  - Department of Pathology, School of Basic Medicine, Xinjiang Medical University, 
      Urumqi, China. yangyun1991kyzy@163.com.
FAU - Hasimu, Axiangu
AU  - Hasimu A
AD  - Department of Pathology, School of Basic Medicine, Xinjiang Medical University, 
      Urumqi, China. hasimu19@163.com.
LA  - eng
PT  - Journal Article
DEP - 20200205
PL  - Bosnia and Herzegovina
TA  - Bosn J Basic Med Sci
JT  - Bosnian journal of basic medical sciences
JID - 101200947
RN  - 0 (Biomarkers)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers/metabolism
MH  - Carcinoma, Squamous Cell/diagnosis/*metabolism/virology
MH  - Case-Control Studies
MH  - Female
MH  - Humans
MH  - Metabolomics
MH  - Middle Aged
MH  - Papillomavirus Infections/*complications/diagnosis/*metabolism
MH  - Predictive Value of Tests
MH  - Uterine Cervical Neoplasms/diagnosis/*metabolism/*virology
MH  - Uterine Cervical Dysplasia/diagnosis/*metabolism/virology
PMC - PMC7029203
COIS- Conflict of interest statement: The authors declare no conflict of interests
EDAT- 2019/08/30 06:00
MHDA- 2020/12/19 06:00
PMCR- 2020/02/01
CRDT- 2019/08/30 06:00
PHST- 2019/07/15 00:00 [received]
PHST- 2019/08/27 00:00 [accepted]
PHST- 2019/08/30 06:00 [pubmed]
PHST- 2020/12/19 06:00 [medline]
PHST- 2019/08/30 06:00 [entrez]
PHST- 2020/02/01 00:00 [pmc-release]
AID - BJBMS-20-78 [pii]
AID - 10.17305/bjbms.2019.4359 [doi]
PST - epublish
SO  - Bosn J Basic Med Sci. 2020 Feb 5;20(1):78-87. doi: 10.17305/bjbms.2019.4359.