PMID- 31468481
OWN - NLM
STAT- MEDLINE
DCOM- 20201013
LR  - 20201013
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2050
DP  - 2020
TI  - Nanoelectroporation and Collection of Genetically Modified Exosomes in Primary 
      Cultures of Dendritic Cells.
PG  - 79-84
LID - 10.1007/978-1-4939-9740-4_8 [doi]
AB  - Dendritic cells (DCs) are cells of the immune system that behave as antigen 
      presenters and assist in T cell activation. DCs have recently been used in 
      cell-based immunotherapies for the treatment of different diseases due to the 
      lack of adverse nonspecific immune responses, typically elicited by other 
      approaches. Genetically modified DCs, for example, have been used to stimulate 
      CD4/CD8 antigen presenting immune responses. However, genetic manipulation of 
      primary DCs remains a challenge. Here we describe a protocol for nonviral, benign 
      transfection of primary DCs using nanochannel-based electroporation, and the 
      subsequent collection of genetically modified exosomes for downstream 
      applications.
FAU - Duarte-Sanmiguel, Silvia
AU  - Duarte-Sanmiguel S
AD  - Department of Biomedical Engineering, The Ohio State University, Columbus, OH, 
      USA.
AD  - Department of Surgery, The Ohio State University, Columbus, OH, USA.
FAU - Higuita-Castro, Natalia
AU  - Higuita-Castro N
AD  - Department of Biomedical Engineering, The Ohio State University, Columbus, OH, 
      USA.
AD  - Department of Surgery, The Ohio State University, Columbus, OH, USA.
FAU - Gallego-Perez, Daniel
AU  - Gallego-Perez D
AD  - Department of Biomedical Engineering, The Ohio State University, Columbus, OH, 
      USA. gallegoperez.1@osu.edu.
AD  - Department of Surgery, The Ohio State University, Columbus, OH, USA. 
      gallegoperez.1@osu.edu.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (CD4 Antigens)
RN  - 0 (CD8 Antigens)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - CD4 Antigens/metabolism
MH  - CD8 Antigens/metabolism
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/immunology
MH  - Electroporation/*instrumentation
MH  - Exosomes/*genetics/transplantation
MH  - Humans
MH  - Mice
MH  - Nanotechnology
MH  - Primary Cell Culture/*methods
MH  - Transfection/*instrumentation
OTO - NOTNLM
OT  - Cell-based immunotherapies
OT  - Dendritic cells
OT  - Nanochannel-based electroporation
EDAT- 2019/08/31 06:00
MHDA- 2020/10/21 06:00
CRDT- 2019/08/31 06:00
PHST- 2019/08/31 06:00 [entrez]
PHST- 2019/08/31 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
AID - 10.1007/978-1-4939-9740-4_8 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2020;2050:79-84. doi: 10.1007/978-1-4939-9740-4_8.