PMID- 31468622
OWN - NLM
STAT- MEDLINE
DCOM- 20191213
LR  - 20200108
IS  - 1099-0461 (Electronic)
IS  - 1095-6670 (Linking)
VI  - 33
IP  - 10
DP  - 2019 Oct
TI  - Changes in the expression levels of CB1 and GLP-1R mRNAs and microRNAs 33a and 
      122 in the liver of type 2 diabetic rats treated with ghrelin.
PG  - e22388
LID - 10.1002/jbt.22388 [doi]
AB  - The aim of the study is to clarify the effect of ghrelin treatment on the 
      messenger RNA (mRNA) expression of the cannabinoid receptor 1 (Cnr1/CB1) and 
      glucagon-like peptide 1 receptor (Glp1r/GLP-1R) as well as microRNAs (miR)-122 
      and miR-33a in the liver of rats with type 2 diabetes mellitus (T2DM). Adult 
      Sprague-Dawley rats were divided into three groups: control (n = 7), T2DM 
      (n = 7), and treatment (n = 7). Control animals received tap water. T2DM was 
      induced by feeding 10% fructose in drinking water for 2 weeks followed by a 
      single injection of streptozotocin (40 mg/kg, intraperitoneally [IP]). In the 
      treatment group, diabetic rats were injected ghrelin (25 mug/kg, IP) for 14 days. 
      Serum lipid profiles were evaluated, and mRNA expression levels of Cnr1 and Glp1r 
      in the liver were detected using quantitative real-time polymerase chain reaction 
      (RT-qPCR). In addition, miR-122 and miR-33a levels were measured using RT-qPCR. 
      Serum triglycerides, low-density lipoprotein cholesterol, and very-low-density 
      lipoprotein cholesterol significantly increased in the T2DM group compared with 
      control rats but ghrelin treatment showed no effect on serum lipid levels. The 
      mRNA expression levels of Cnr1 and Glp1r decreased in the T2DM group compared 
      with the control group. These reductions were significantly increased in the T2DM 
      group treated with ghrelin. Furthermore, the increase in miR-33a expression level 
      was reduced in the treatment group compared to rats with T2DM. Our findings 
      suggested that ghrelin treatment may alter the mRNA expression levels of CB1 and 
      GLP-1R in the liver of rats with T2DM. The mRNA levels of Cnr1 and Glp1r may 
      inversely correlate with the expression level of miR-33a but not miR-122.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Coskun, Zeynep M
AU  - Coskun ZM
AUID- ORCID: 0000-0003-4791-6537
AD  - Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, 
      Demiroglu Bilim University, Istanbul, Turkey.
FAU - Beydogan, Alisa B
AU  - Beydogan AB
AUID- ORCID: 0000-0002-8575-536X
AD  - Department of Medical Biology, Faculty of Cerrahpasa Medicine, Istanbul 
      University-Cerrahpasa, Istanbul, Turkey.
FAU - Bolkent, Sema
AU  - Bolkent S
AUID- ORCID: 0000-0001-8463-5561
AD  - Department of Medical Biology, Faculty of Cerrahpasa Medicine, Istanbul 
      University-Cerrahpasa, Istanbul, Turkey.
LA  - eng
PT  - Journal Article
DEP - 20190830
PL  - United States
TA  - J Biochem Mol Toxicol
JT  - Journal of biochemical and molecular toxicology
JID - 9717231
RN  - 0 (Ghrelin)
RN  - 0 (Glucagon-Like Peptide-1 Receptor)
RN  - 0 (MIRN122 microRNA, rat)
RN  - 0 (MIRN33 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, Cannabinoid, CB1)
SB  - IM
MH  - Animals
MH  - Diabetes Mellitus, Type 2/*genetics
MH  - Ghrelin/*administration & dosage
MH  - Glucagon-Like Peptide-1 Receptor/*genetics
MH  - Liver/*metabolism
MH  - MicroRNAs/*genetics
MH  - RNA, Messenger/*genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, Cannabinoid, CB1/*genetics
OTO - NOTNLM
OT  - ghrelin
OT  - liver
OT  - mRNA expression
OT  - microRNA
OT  - type 2 diabetes mellitus
EDAT- 2019/08/31 06:00
MHDA- 2019/12/18 06:00
CRDT- 2019/08/31 06:00
PHST- 2019/04/17 00:00 [received]
PHST- 2019/05/29 00:00 [revised]
PHST- 2019/08/12 00:00 [accepted]
PHST- 2019/08/31 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/08/31 06:00 [entrez]
AID - 10.1002/jbt.22388 [doi]
PST - ppublish
SO  - J Biochem Mol Toxicol. 2019 Oct;33(10):e22388. doi: 10.1002/jbt.22388. Epub 2019 
      Aug 30.