PMID- 31469264
OWN - NLM
STAT- MEDLINE
DCOM- 20201001
LR  - 20201001
IS  - 1520-5010 (Electronic)
IS  - 0893-228X (Linking)
VI  - 32
IP  - 10
DP  - 2019 Oct 21
TI  - In Situ Fluorescence Tracking Toxic Metabolite Mono-2-ethylhexyl phthalate (MEHP) 
      of Di-(2-ethylhexyl) phthalate (DEHP) in HeLa Cells.
PG  - 2006-2015
LID - 10.1021/acs.chemrestox.9b00191 [doi]
AB  - In this study, we synthesized a small molecule fluorescent probe for detecting 
      mono-2-ethylhexyl phthalate (MEHP) named MEHP-AF, which formed by MEHP 
      cross-linked with 5-aminofluorescein (5-AF) through amide bond. MEHP-AF had been 
      purified based on the different physicochemical properties of 5-AF with MEHP. 
      MEHP-AF showed fluorescence characteristics coming from 5-AF and liposoluble 
      property coming from MEHP. After physicochemical characterization, a series of 
      biological studies of its action in cells were carried out. The results indicated 
      that MEHP-AF was a fluorescent probe with strong specificity and high 
      sensitivity. It can visibly track the location of MEHP in HeLa cell or 
      subcellular levels under confocal laser scanning microscopy in situ. This novel 
      fluorescent probe is expected to use for studying its intracellular behavior at 
      the cell level, especially for investigating the interaction between MEHP and 
      cellular molecules.
FAU - Huo, Shao-Jie
AU  - Huo SJ
AD  - College of Life Sciences , Nanjing Normal University , Nanjing 210046 , The 
      People's Republic of China.
FAU - Wu, Xiu
AU  - Wu X
AD  - College of Life Sciences , Nanjing Normal University , Nanjing 210046 , The 
      People's Republic of China.
FAU - Ye, Chong
AU  - Ye C
AD  - College of Life Sciences , Nanjing Normal University , Nanjing 210046 , The 
      People's Republic of China.
FAU - Hu, Meng-Yuan
AU  - Hu MY
AD  - College of Food and Pharmaceutical Engineering , Nanjing Normal University , 
      Nanjing 210046 , The People's Republic of China.
FAU - Li, Wen-Jie
AU  - Li WJ
AD  - College of Food and Pharmaceutical Engineering , Nanjing Normal University , 
      Nanjing 210046 , The People's Republic of China.
FAU - Zhang, Ling-Ling
AU  - Zhang LL
AD  - College of Food and Pharmaceutical Engineering , Nanjing Normal University , 
      Nanjing 210046 , The People's Republic of China.
FAU - Xiang, Su-Yun
AU  - Xiang SY
AD  - College of Life Sciences , Nanjing Normal University , Nanjing 210046 , The 
      People's Republic of China.
AD  - College of Food and Pharmaceutical Engineering , Nanjing Normal University , 
      Nanjing 210046 , The People's Republic of China.
FAU - Yu, Shu-Qin
AU  - Yu SQ
AUID- ORCID: 0000-0002-2824-4651
AD  - College of Life Sciences , Nanjing Normal University , Nanjing 210046 , The 
      People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190911
PL  - United States
TA  - Chem Res Toxicol
JT  - Chemical research in toxicology
JID - 8807448
RN  - 0 (Fluorescent Dyes)
RN  - C42K0PH13C (Diethylhexyl Phthalate)
RN  - FU2EWB60RT (mono-(2-ethylhexyl)phthalate)
SB  - IM
MH  - Diethylhexyl Phthalate/*analogs & derivatives/analysis/*metabolism/toxicity
MH  - Fluorescent Dyes/chemical synthesis/chemistry
MH  - HeLa Cells
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Structure
MH  - Tumor Cells, Cultured
EDAT- 2019/08/31 06:00
MHDA- 2020/10/02 06:00
CRDT- 2019/08/31 06:00
PHST- 2019/08/31 06:00 [pubmed]
PHST- 2020/10/02 06:00 [medline]
PHST- 2019/08/31 06:00 [entrez]
AID - 10.1021/acs.chemrestox.9b00191 [doi]
PST - ppublish
SO  - Chem Res Toxicol. 2019 Oct 21;32(10):2006-2015. doi: 
      10.1021/acs.chemrestox.9b00191. Epub 2019 Sep 11.