PMID- 31471398
OWN - NLM
STAT- MEDLINE
DCOM- 20200128
LR  - 20200128
IS  - 1791-7549 (Electronic)
IS  - 0258-851X (Print)
IS  - 0258-851X (Linking)
VI  - 33
IP  - 5
DP  - 2019 Sep-Oct
TI  - The Role of Fgf Signaling on Epithelial Cell Differentiation in Mouse Vagina.
PG  - 1499-1505
LID - 10.21873/invivo.11630 [doi]
AB  - BACKGROUND/AIM: The mouse vagina exhibits stratified squamous epithelium, which 
      is comprised of multiple cell layers. We previously showed that erbB signaling, 
      induced by epithelial estrogen receptor 1 (ESR1), is required for the initial 
      differentiation of the epithelium. However, the downstream effector that mediates 
      terminal differentiation in the apical layers remains elusive. The contribution 
      of fibroblast growth factor (FGF) to vaginal epithelial cell differentiation was 
      investigated. MATERIALS AND METHODS: Vaginas from wild-type or 
      epithelium-specific Esr1 conditional knockout (cKO) mice were analyzed using 
      immunohistochemistry and quantitative real-time RT-PCR. RESULTS: Of the FGF 
      ligands examined, Fgf22 mRNA was significantly induced following estrogen 
      treatment. Furthermore, FGF downstream signaling, phosphorylated FRS2 and ERK1/2 
      were exclusively expressed in the apical layers of the vaginal epithelium. No 
      changes in such expression were observed in the Esr1 cKO mice. CONCLUSION: 
      FGF-ERK/MAPK pathway may be a main inducer of terminal differentiation in the 
      mouse vaginal epithelium.
CI  - Copyright(c) 2019, International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Hirano, Y U
AU  - Hirano YU
AD  - Department of Developmental Genetics, Institute of Advanced Medicine, Wakayama 
      Medical University, Wakayama, Japan.
FAU - Suzuki, Kentaro
AU  - Suzuki K
AD  - Department of Developmental Genetics, Institute of Advanced Medicine, Wakayama 
      Medical University, Wakayama, Japan.
FAU - Iguchi, Taisen
AU  - Iguchi T
AD  - Graduate School of Nanobioscience, Yokohama City University, Yokohama, Japan.
FAU - Yamada, Gen
AU  - Yamada G
AD  - Department of Developmental Genetics, Institute of Advanced Medicine, Wakayama 
      Medical University, Wakayama, Japan miyagawa@rs.tus.ac.jp genyama77@yahoo.co.jp.
FAU - Miyagawa, Shinichi
AU  - Miyagawa S
AD  - Faculty of Industrial Science and Technology, Tokyo University of Science, Tokyo, 
      Japan miyagawa@rs.tus.ac.jp genyama77@yahoo.co.jp.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - In Vivo
JT  - In vivo (Athens, Greece)
JID - 8806809
RN  - 0 (Biomarkers)
RN  - 0 (Ligands)
RN  - 62031-54-3 (Fibroblast Growth Factors)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - *Cell Differentiation/genetics
MH  - Epithelial Cells/*cytology/*metabolism
MH  - Female
MH  - Fibroblast Growth Factors/*metabolism
MH  - Ligands
MH  - Mice
MH  - Mice, Transgenic
MH  - Mucous Membrane/*cytology/*metabolism
MH  - Signal Transduction
MH  - *Vagina
PMC - PMC6755014
OTO - NOTNLM
OT  - Estrogen receptor
OT  - epithelium
OT  - fibroblast growth factors
OT  - keratinization
OT  - vagina
COIS- None of the Authors has any potential conflicts of interest associated with this 
      research.
EDAT- 2019/09/01 06:00
MHDA- 2020/01/29 06:00
PMCR- 2019/09/03
CRDT- 2019/09/01 06:00
PHST- 2019/05/26 00:00 [received]
PHST- 2019/06/30 00:00 [revised]
PHST- 2019/07/03 00:00 [accepted]
PHST- 2019/09/01 06:00 [entrez]
PHST- 2019/09/01 06:00 [pubmed]
PHST- 2020/01/29 06:00 [medline]
PHST- 2019/09/03 00:00 [pmc-release]
AID - 33/5/1499 [pii]
AID - 10.21873/invivo.11630 [doi]
PST - ppublish
SO  - In Vivo. 2019 Sep-Oct;33(5):1499-1505. doi: 10.21873/invivo.11630.