PMID- 31472263
OWN - NLM
STAT- MEDLINE
DCOM- 20200204
LR  - 20200204
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 94
DP  - 2019 Nov
TI  - A sialic acid-binding lectin with bactericidal and opsonic activities from 
      Ruditapes philippinarum.
PG  - 72-80
LID - S1050-4648(19)30874-5 [pii]
LID - 10.1016/j.fsi.2019.08.074 [doi]
AB  - In the present study, a sialic acid-binding lectin was cloned and characterized 
      from Manila clam Ruditapes philippinarum (designed as RpSabl). The open reading 
      frame of RpSabl encoded a polypeptide of 162 amino acids with a calculated 
      molecular mass of 17.7 kDa. Analysis of the conserved domain suggested that 
      RpSabl was a new member of the sialic acid-binding lectins family. In 
      non-stimulated clams, RpSabl transcripts were constitutively expressed in all 
      five tested tissues, especially in hepatopancreas. After Vibrio anguillarum 
      challenge, the expression of RpSabl mRNA in hepatopancreas was significantly 
      up-regulated at 3 h (3.8-fold, P < 0.05), 6 h (4.9-fold, P < 0.05), 12 h 
      (12.3-fold, P < 0.01) and 24 h (9.7-fold, P < 0.01), while RpSabl transcripts in 
      hemocytes was only significantly up-regulated at 6 h (8.5-Fold, P < 0.01). 
      RNAi-mediated knockdown of RpSabl transcripts affected the survival rates of 
      Manila clam against V. anguillarum, perhaps mainly due to the inhibited 
      expression of antibacterial effectors (e.g. lysozyme and defensin). Moreover, 
      recombinant protein of RpSabl (rRpSabl) possessed binding activities towards 
      lipopolysaccharides (LPS), peptidoglycan (PGN) and glucan in vitro. Coinciding 
      with the Pathogen-associated molecular patterns (PAMPs) binding assay, rRpSabl 
      displayed broad bacterial-agglutination properties towards Vibrio harveyi, Vibrio 
      splendidus, V. anguillarum, Enterobacter cloacae and Aeromonas hydrophila. 
      Meanwhile, the phagocytosis and encapsulation ability of hemocytes could be 
      significantly enhanced by rRpSabl incubation. All these results showed that 
      RpSabl could function as a versatile molecule involved in the innate immune 
      responses of R. philippinarum.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Zhang, Jianning
AU  - Zhang J
AD  - School of Life Sciences, Ludong University, Yantai, 264025, PR China.
FAU - Zhang, Yifei
AU  - Zhang Y
AD  - School of Life Sciences, Ludong University, Yantai, 264025, PR China.
FAU - Zhang, Linbao
AU  - Zhang L
AD  - Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South 
      China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 
      Guangzhou, 510300, PR China.
FAU - Wei, Qianyu
AU  - Wei Q
AD  - Center for Ocean Mega-science, Chinese Academy of Sciences, Qingdao, Shandong, 
      266071, PR China.
FAU - Liu, Xiaoli
AU  - Liu X
AD  - School of Life Sciences, Ludong University, Yantai, 264025, PR China; The Coastal 
      Resources and Environment Team for Blue-Yellow Area, Ludong University, Yantai, 
      264025, PR China. Electronic address: lxlshz2006@163.com.
FAU - Yang, Dinglong
AU  - Yang D
AD  - Muping Coastal Environment Research Station, Yantai Institute of Coastal Zone 
      Research, Chinese Academy of Sciences, Yantai, 264003, PR China; Center for Ocean 
      Mega-science, Chinese Academy of Sciences, Qingdao, Shandong, 266071, PR China. 
      Electronic address: dlyang@yic.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20190828
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Lectins)
RN  - 0 (Opsonin Proteins)
RN  - 0 (Sialic Acids)
SB  - IM
MH  - Aeromonas hydrophila/drug effects
MH  - Amino Acid Sequence
MH  - Animals
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Bivalvia/*genetics/*immunology
MH  - Enterobacter cloacae/drug effects
MH  - Lectins/chemistry/*genetics/metabolism
MH  - Opsonin Proteins/*pharmacology
MH  - Sequence Alignment
MH  - Sialic Acids/*metabolism
MH  - Vibrio/drug effects
OTO - NOTNLM
OT  - Immune recognition
OT  - Pattern recognition receptor
OT  - Ruditapes philippinarum
OT  - Sialic acid-binding lectin
EDAT- 2019/09/01 06:00
MHDA- 2020/02/06 06:00
CRDT- 2019/09/01 06:00
PHST- 2019/06/15 00:00 [received]
PHST- 2019/08/08 00:00 [revised]
PHST- 2019/08/28 00:00 [accepted]
PHST- 2019/09/01 06:00 [pubmed]
PHST- 2020/02/06 06:00 [medline]
PHST- 2019/09/01 06:00 [entrez]
AID - S1050-4648(19)30874-5 [pii]
AID - 10.1016/j.fsi.2019.08.074 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2019 Nov;94:72-80. doi: 10.1016/j.fsi.2019.08.074. Epub 
      2019 Aug 28.