PMID- 31472264
OWN - NLM
STAT- MEDLINE
DCOM- 20200207
LR  - 20200207
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 94
DP  - 2019 Nov
TI  - Florfenicol alleviated lipopolysaccharide (LPS)-induced inflammatory responses in 
      Ctenopharyngodon idella through inhibiting toll / NF-kappaB signaling pathways.
PG  - 479-484
LID - S1050-4648(19)30873-3 [pii]
LID - 10.1016/j.fsi.2019.08.073 [doi]
AB  - The present study was conducted to evaluate the anti-inflammatory activity of 
      florfenicol (FFC) against lipopolysaccharide (LPS)-induced inflammatory responses 
      in Ctenopharyngodon idella in vivo and in vitro. Head-kidney (HK) macrophages 
      were pre-treated with 10 mug/mL LPS and then exposed to different concentrations 
      of FFC to determine its in vitro anti-inflammatory activity. Inhibitory effect of 
      FFC on inflammatory mediators TNF-alpha, IL-6 and IL-1beta, as well as LPS-induced 
      nitric oxide (NO) and prostaglandin E 2 (PGE 2) production were assayed by ELISA. 
      The expression level of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) 
      were investigated by RT-PCR. Expression level of TLR-related genes (TLR1, TLR2, 
      TLR4, TLR7, TLR8) expression, tumor necrosis factor receptor-associated factor 6 
      (TRAF6), transforming growth factor-b-activated kinase 1 (TAK1), Myeloid 
      differentiation factor 88 (MyD88), nucleus p65, NF-kappaBalpha (IkappaBalpha) were measured by 
      RT-PCR after grass carp were treated with 50, 100 and 200 mg FFC/kg body weight 
      for 3 days. Results from in vitro tests demonstrated that FFC dose-dependently 
      inhibited LPS-induced inflammatory cytokines TNF-alpha, IL-6 and IL-1beta, inflammatory 
      factors NO and PGE 2 production in macrophages. In addition, iNOS and COX-2 
      expression levels decreased significantly as compared with LPS treated group. In 
      vivo test demonstrated that treatment with FFC prevented the LPS-induced 
      upregulation of TNF-alpha, IL-6, IL-1beta, NO and PGE 2. The expression level of iNOS, 
      and COX-2 in FFC-treated grass carp were also downregulated as compared with LPS 
      treated fish. Besides, FFC blocked the expression of Toll-like receptor 2 (TLR2) 
      and then suppressed the phosphorylation of nuclear transcription factor-kappa B 
      (NF-kappaB) p65 and degradation inhibitor of IkappaBalpha. Furthermore, administration of FFC 
      inhibited the up-regulation of IRAK4, TRAF6 and TAK1 induced by LPS. These 
      results suggest that the anti-inflammatory properties of FFC might be the results 
      from the inhibition of iNOS, COX-2, IL-6, IL-1beta, and TNF-alpha expressions through 
      the down-regulation of Toll/NF-kappaB signaling pathways.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Li, Pei
AU  - Li P
AD  - Center for Food Quality Supervision and Testing (Zhanjiang)Ministry of 
      Agriculture and Rural Affairs PR China, Agricultural Products Processing Research 
      Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, 52400, 
      China; Institute for Fisheries Sciences, Guangxi University, Nanning, 53000, 
      China.
FAU - Ye, Jianzhi
AU  - Ye J
AD  - Center for Food Quality Supervision and Testing (Zhanjiang)Ministry of 
      Agriculture and Rural Affairs PR China, Agricultural Products Processing Research 
      Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, 52400, 
      China.
FAU - Zeng, Shaodong
AU  - Zeng S
AD  - Center for Food Quality Supervision and Testing (Zhanjiang)Ministry of 
      Agriculture and Rural Affairs PR China, Agricultural Products Processing Research 
      Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, 52400, 
      China.
FAU - Yang, Chunliang
AU  - Yang C
AD  - Center for Food Quality Supervision and Testing (Zhanjiang)Ministry of 
      Agriculture and Rural Affairs PR China, Agricultural Products Processing Research 
      Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, 52400, 
      China. Electronic address: 3403009643@qq.com.
LA  - eng
PT  - Journal Article
DEP - 20190828
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Toll-Like Receptors)
RN  - 9J97307Y1H (florfenicol)
RN  - FLQ7571NPM (Thiamphenicol)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Carps/genetics/*immunology
MH  - Dose-Response Relationship, Drug
MH  - Fish Diseases/chemically induced/*drug therapy
MH  - Inflammation/chemically induced/drug therapy/*veterinary
MH  - Lipopolysaccharides/pharmacology
MH  - NF-kappa B/physiology
MH  - Random Allocation
MH  - Signal Transduction/drug effects/physiology
MH  - Thiamphenicol/*analogs & derivatives/pharmacology
MH  - Toll-Like Receptors/physiology
OTO - NOTNLM
OT  - Ctenopharyngodon idella
OT  - Florfenicol
OT  - Inflammatory responses
OT  - Toll/NF-kappaB signaling pathways
OT  - lipopolysaccharide
EDAT- 2019/09/01 06:00
MHDA- 2020/02/08 06:00
CRDT- 2019/09/01 06:00
PHST- 2019/06/26 00:00 [received]
PHST- 2019/08/25 00:00 [revised]
PHST- 2019/08/27 00:00 [accepted]
PHST- 2019/09/01 06:00 [pubmed]
PHST- 2020/02/08 06:00 [medline]
PHST- 2019/09/01 06:00 [entrez]
AID - S1050-4648(19)30873-3 [pii]
AID - 10.1016/j.fsi.2019.08.073 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2019 Nov;94:479-484. doi: 10.1016/j.fsi.2019.08.073. Epub 
      2019 Aug 28.