PMID- 31472699
OWN - NLM
STAT- MEDLINE
DCOM- 20191216
LR  - 20191217
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 1082
DP  - 2019 Nov 15
TI  - Simultaneous quantitative analysis of multiple sphingoid bases by stable isotope 
      labeling assisted liquid chromatography-mass spectrometry.
PG  - 106-115
LID - S0003-2670(19)30822-0 [pii]
LID - 10.1016/j.aca.2019.07.016 [doi]
AB  - Sphingoid bases (SBs) are one of important components of cell membranes, playing 
      important roles in cellular biology. Meanwhile, SBs are associated with various 
      metabolic diseases such as Type 2 Diabetes mellitus (T2DM). Therefore, 
      simultaneous quantitation of multiple SBs in biological samples could provide 
      crucial information for uncovering underlying mechanisms of SBs related functions 
      and diseases. However, existing methods are difficult to achieve simultaneous 
      quantitation for multiple SBs due to the lack of isotope internal standards (ISs) 
      of corresponding SBs. In the current study, we developed a highly sensitive 
      method for the simultaneous detection of 26 SBs in biological samples by stable 
      isotope labeling coupled with ultra-high performance liquid chromatography tandem 
      mass spectrometry (SIL-UHPLC-MS/MS) analysis. In this respect, a pair of isotope 
      labeling reagents, 3-(N, N-dimethylamino)propyl isothiocyanate (DMPI) and 
      d(4)-3-(N, N-dimethylamino)propyl isothiocyanate (d(4)-DMPI), were synthesized 
      and utilized to label SBs in biological samples and SB standards, respectively. 
      The d(4)-DMPI labeled SB standards were used as ISs to calibrate quantitation 
      deviation in MS analysis from the biological matrix. Using the developed method, 
      we successfully quantitated 19 SBs in cells, 20 SBs in mice feces and 18 SBs in 
      human serum samples. Three C17-SBs used as ISs in many reported works were even 
      found in all prepared samples. In summary, the developed SIL-UHPLC-MS/MS analysis 
      was demonstrated to be a promising method for the simultaneous determination of 
      multiple SBs, which could facilitate the investigation of cellular function of 
      SBs and pathogenesis of related diseases.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Zheng, Shu-Jian
AU  - Zheng SJ
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, 430072, PR China.
FAU - Zheng, Jie
AU  - Zheng J
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, 430072, PR China.
FAU - Xiao, Hua-Ming
AU  - Xiao HM
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, 430072, PR China.
FAU - Wu, Dong-Mei
AU  - Wu DM
AD  - Department of Oncology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, PR 
      China.
FAU - Feng, Yu-Qi
AU  - Feng YQ
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, 430072, PR China. 
      Electronic address: yqfeng@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190709
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Biomarkers)
RN  - 0 (Isothiocyanates)
RN  - 0 (Sphingolipids)
RN  - AR09D82C7G (Deuterium)
SB  - IM
MH  - Alzheimer Disease/diagnosis
MH  - Animals
MH  - Biomarkers/blood/chemistry
MH  - Cell Line, Tumor
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Deuterium
MH  - Diabetes Mellitus, Type 2/diagnosis
MH  - Feces/chemistry
MH  - Humans
MH  - Isothiocyanates/chemistry
MH  - Isotope Labeling/methods
MH  - Limit of Detection
MH  - Mice
MH  - Sphingolipids/*blood/chemistry
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Alzheimer's disease
OT  - Cells
OT  - Liquid chromatography-tandem mass spectrometry
OT  - Sphingoid bases
OT  - Stable isotope labeling
OT  - T2DM
EDAT- 2019/09/02 06:00
MHDA- 2019/12/18 06:00
CRDT- 2019/09/02 06:00
PHST- 2019/05/10 00:00 [received]
PHST- 2019/07/05 00:00 [revised]
PHST- 2019/07/07 00:00 [accepted]
PHST- 2019/09/02 06:00 [entrez]
PHST- 2019/09/02 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
AID - S0003-2670(19)30822-0 [pii]
AID - 10.1016/j.aca.2019.07.016 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2019 Nov 15;1082:106-115. doi: 10.1016/j.aca.2019.07.016. Epub 
      2019 Jul 9.