PMID- 31473865 OWN - NLM STAT- MEDLINE DCOM- 20200323 LR - 20210110 IS - 1436-2236 (Electronic) IS - 1436-2228 (Linking) VI - 21 IP - 6 DP - 2019 Dec TI - A Molecular Cytogenetic Map of Scallop (Patinopecten yessoensis). PG - 731-742 LID - 10.1007/s10126-019-09918-6 [doi] AB - To consolidate the genetic, physical, and cytogenetic maps of scallop (Patinopecten yessoensis), we constructed a molecular cytogenetic map by localizing 84 fosmid clones that contain different SNP markers from 19 linkage groups (LGs) using fluorescence in situ hybridization (FISH). Among these 84 SNP-anchored clones, 56 clones produced specific and stable signals on one pair of chromosomes. Dual-color FISH assigned 19 LGs to their corresponding chromosomes with 38 SNP-anchored clones as probes. Among these 19 LGs, 17 LGs were assigned to their corresponding one pair of chromosomes, while two clones containing SNPs from LG10 and LG19 were located on two different pairs of chromosomes separately. The orientation of 7 LGs was corrected according to the chromosome location of SNPs within the same LG. In addition, a probe panel of SNP-anchored clones was developed to identify each chromosome of P. yessoensis. The molecular cytogenetic map will facilitate molecular breeding in scallop and enable comparative studies on chromosome evolution of bivalve mollusk. FAU - Yang, Zujing AU - Yang Z AD - MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China. AD - Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. FAU - Li, Xuan AU - Li X AD - MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China. AD - Groningen Institute for Evolutionary Life Sciences, University of Groningen, Post Office Box 11103, 9700 CC, Groningen, Netherlands. FAU - Liao, Huan AU - Liao H AD - MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China. AD - College of Animal Biotechnology, Jiangxi Agricultural University, Nanchang, China. FAU - Hu, Liping AU - Hu L AD - MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China. AD - Yantai Fisheries Research Institute, Yantai, China. FAU - Peng, Cheng AU - Peng C AD - MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China. FAU - Wang, Shenhai AU - Wang S AD - MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China. FAU - Huang, Xiaoting AU - Huang X AD - MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China. xthuang@ouc.edu.cn. AD - Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. xthuang@ouc.edu.cn. FAU - Bao, Zhenmin AU - Bao Z AD - MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China. AD - Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. LA - eng GR - 2018YFD0901402 and 2018YFD0900304/National Key R&D Program of China/ GR - CARS-49/Earmarked Fund for Modern Agro-industry Technology Research System/ GR - 2016ZDJQ0208/Key Research and Development Program of Shandong Province/ PT - Journal Article DEP - 20190831 PL - United States TA - Mar Biotechnol (NY) JT - Marine biotechnology (New York, N.Y.) JID - 100892712 SB - IM MH - Animals MH - *Chromosome Mapping MH - Chromosomes MH - Genetic Linkage MH - In Situ Hybridization, Fluorescence MH - Pectinidae/*genetics MH - Polymorphism, Single Nucleotide OTO - NOTNLM OT - Cytogenetic map OT - FISH OT - Fosmid clone OT - Genetic map OT - Scallop EDAT- 2019/09/02 06:00 MHDA- 2020/03/24 06:00 CRDT- 2019/09/02 06:00 PHST- 2019/04/17 00:00 [received] PHST- 2019/08/12 00:00 [accepted] PHST- 2019/09/02 06:00 [pubmed] PHST- 2020/03/24 06:00 [medline] PHST- 2019/09/02 06:00 [entrez] AID - 10.1007/s10126-019-09918-6 [pii] AID - 10.1007/s10126-019-09918-6 [doi] PST - ppublish SO - Mar Biotechnol (NY). 2019 Dec;21(6):731-742. doi: 10.1007/s10126-019-09918-6. Epub 2019 Aug 31.