PMID- 31478827
OWN - NLM
STAT- MEDLINE
DCOM- 20200604
LR  - 20200604
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 100
IP  - 10
DP  - 2019 Oct
TI  - Cellular microRNA miR-c89 inhibits replication of porcine reproductive and 
      respiratory syndrome virus by targeting the host factor porcine retinoid X 
      receptor beta.
PG  - 1407-1416
LID - 10.1099/jgv.0.001320 [doi]
AB  - MicroRNAs (miRNAs) play critical roles in the complex networks of virus-host 
      interactions. Our previous research showed that porcine reproductive and 
      respiratory syndrome virus (PRRSV) infection markedly upregulates miR-c89 
      expression, suggesting that miR-c89 may play an important role in PRRSV 
      infection. The present study sought to determine the function of miR-c89 and its 
      molecular mechanism during PRRSV infection. Using quantitative reverse 
      transcription PCR (RT-qPCR) verification, we demonstrated that both highly 
      pathogenic PRRSV and low-pathogenic PRRSV infection induced miR-c89 expression. 
      The overexpression of miR-c89 significantly suppressed the replication of a 
      variety of PRRSV strains, regardless of the timing of infection. Further, miR-c89 
      can directly target the 3'UTR of porcine retinoid X receptor beta (RXRB) mRNA in a 
      sequence-specific manner. Knockdown affected RXRB expression, as siRNA can 
      suppress the replication of a variety of PRRSV strains. This work not only 
      provides new insights into PRRSV-cell interactions, but also highlights the 
      potential for the use of miR-c89 in the development of new antiviral strategies 
      to combat PRRSV infection.
FAU - Zhang, Xiaobin
AU  - Zhang X
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      712100, PR China.
FAU - Feng, Yingtong
AU  - Feng Y
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      712100, PR China.
FAU - Yan, Yunhuan
AU  - Yan Y
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      712100, PR China.
FAU - Zheng, Zifang
AU  - Zheng Z
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      712100, PR China.
FAU - Wang, Wenjing
AU  - Wang W
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      712100, PR China.
FAU - Zhang, Yichi
AU  - Zhang Y
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      712100, PR China.
FAU - Zhou, En-Min
AU  - Zhou EM
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      712100, PR China.
FAU - Xiao, Shuqi
AU  - Xiao S
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      712100, PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190903
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (3' Untranslated Regions)
RN  - 0 (MicroRNAs)
RN  - 0 (Retinoid X Receptor beta)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Animals
MH  - Cell Line
MH  - Host-Pathogen Interactions
MH  - MicroRNAs/genetics/metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/genetics/*metabolism/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*physiology
MH  - Retinoid X Receptor beta/genetics/*metabolism
MH  - Swine
MH  - Virus Replication
OTO - NOTNLM
OT  - PRRSV
OT  - RXRB
OT  - miRNA
OT  - post-transcriptional regulation
OT  - virus-cell interaction
EDAT- 2019/09/04 06:00
MHDA- 2020/06/05 06:00
CRDT- 2019/09/04 06:00
PHST- 2019/09/04 06:00 [pubmed]
PHST- 2020/06/05 06:00 [medline]
PHST- 2019/09/04 06:00 [entrez]
AID - 10.1099/jgv.0.001320 [doi]
PST - ppublish
SO  - J Gen Virol. 2019 Oct;100(10):1407-1416. doi: 10.1099/jgv.0.001320. Epub 2019 Sep 
      3.