PMID- 3147897
OWN - NLM
STAT- MEDLINE
DCOM- 19890418
LR  - 20190824
IS  - 0014-2972 (Print)
IS  - 0014-2972 (Linking)
VI  - 18
IP  - 5
DP  - 1988 Oct
TI  - A monoclonal Ro-antibody and the serum of a Ro-positive patient with subacute 
      cutaneous lupus erythematosus (SCLE) react with basal layers of human epidermis.
PG  - 465-71
AB  - Skin lesions, especially at areas exposed to sunlight, prove to be a major form 
      of manifestation of diseases related to Ro-antibodies and neonatal-, 
      'ANA-negative-', and cutaneous types of lupus erythematosus. A monoclonal 
      Ro-antibody established by our group reacts with a 60 kD polypeptide in extracts 
      from human spleen, whereas in extracts from human epidermis the monoclonal 
      Ro-antibody and a purified Ro-antibody from a monospecific serum of a patient 
      with subacute cutaneous lupus erythematosus reacted with a 60 kD and a 48 kD 
      protein. Performing immunofluorescence microscopy on HEp2-cells both antibodies 
      showed a nuclear speckled staining pattern and a reaction with cytokeratin 
      filaments. On skin biopsies obtained from the patient, predominantly the basal 
      layers of the proliferative stratum germinativum demonstrated a high rate of 
      immunofluorescence; antigen-expression seems to depend on sunlight exposure. From 
      these data we assume that, especially in subacute cutaneous lupus erythematosus, 
      cross-reaction of the Ro-antibody with one of the lower molecular keratins 
      happens in that area of human epidermis which histologically shows cellular 
      destruction.
FAU - Mayet, W J
AU  - Mayet WJ
AD  - I. Medizinische Klink, Universitat Mainz, FRG.
FAU - Bachmann, M
AU  - Bachmann M
FAU - Pfeifer, K
AU  - Pfeifer K
FAU - Schroder, H C
AU  - Schroder HC
FAU - Muller, W E
AU  - Muller WE
FAU - Gudat, W
AU  - Gudat W
FAU - Korting, G W
AU  - Korting GW
FAU - Meyer zum Buschenfelde, K H
AU  - Meyer zum Buschenfelde KH
LA  - eng
PT  - Journal Article
PL  - England
TA  - Eur J Clin Invest
JT  - European journal of clinical investigation
JID - 0245331
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (SS-A antibodies)
SB  - IM
MH  - Antibodies, Antinuclear/*immunology
MH  - Antibodies, Monoclonal/*immunology
MH  - Blotting, Western
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epidermis/immunology
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Lupus Erythematosus, Cutaneous/*immunology
EDAT- 1988/10/01 00:00
MHDA- 1988/10/01 00:01
CRDT- 1988/10/01 00:00
PHST- 1988/10/01 00:00 [pubmed]
PHST- 1988/10/01 00:01 [medline]
PHST- 1988/10/01 00:00 [entrez]
AID - 10.1111/j.1365-2362.1988.tb01041.x [doi]
PST - ppublish
SO  - Eur J Clin Invest. 1988 Oct;18(5):465-71. doi: 
      10.1111/j.1365-2362.1988.tb01041.x.