PMID- 31479470
OWN - NLM
STAT- MEDLINE
DCOM- 20200316
LR  - 20200316
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 14
IP  - 9
DP  - 2019
TI  - Methods for analyzing tellurium imaging mass cytometry data.
PG  - e0221714
LID - 10.1371/journal.pone.0221714 [doi]
LID - e0221714
AB  - Imaging mass cytometry (IMC) is a technique allowing visualization and 
      quantification of over 40 biological parameters in a single experiment with 
      subcellular spatial resolution, however most IMC experiments are limited to 
      endpoint analysis with antibodies and DNA stains. Small molecules containing 
      tellurium are promising probes for IMC due to their cell permeability, synthetic 
      versatility, and most importantly their application to sequential labelling with 
      isotopologous probes (SLIP) experiments. SLIP experiments with 
      tellurium-containing probes allow quantification of intracellular biology at 
      multiple timepoints with IMC. Despite the promise of tellurium in IMC, there are 
      unique challenges in image processing associated with tellurium IMC data. Here, 
      we address some of these issues by demonstrating the removal of xenon background 
      signal, combining channels to improve signal-to-noise ratio, and calculating 
      isotope transmission efficiency biases. These developments add accuracy to the 
      unique temporal resolution afforded by tellurium IMC probes.
FAU - Bassan, Jay
AU  - Bassan J
AUID- ORCID: 0000-0003-3682-4629
AD  - Department of Chemistry, University of Toronto, Toronto, Ontario, Canada.
AD  - BIMDAQ Ltd, Bexleyheath, United Kingdom.
FAU - Nitz, Mark
AU  - Nitz M
AD  - Department of Chemistry, University of Toronto, Toronto, Ontario, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190903
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Isotopes)
RN  - 0 (Molecular Probes)
RN  - 0 (Tellurium-124)
RN  - 0 (Tellurium-126)
RN  - 3H3U766W84 (Xenon)
RN  - NQA0O090ZJ (Tellurium)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Image Cytometry/*methods
MH  - Image Processing, Computer-Assisted
MH  - Isotopes/chemistry/pharmacokinetics
MH  - Jejunum/anatomy & histology/metabolism
MH  - Mice
MH  - Molecular Probe Techniques
MH  - *Molecular Probes/chemistry/pharmacokinetics
MH  - Signal-To-Noise Ratio
MH  - Subtraction Technique
MH  - *Tellurium/chemistry/pharmacokinetics
MH  - Xenon/pharmacokinetics
PMC - PMC6719864
COIS- JB owns stock in BIMDAQ Ltd. MN owns intellectual property relating to the use of 
      organotellurium probes in mass cytometry. MN and JB were funded by Fluidigm 
      Canada Inc. This does not alter our adherence to PLOS ONE policies on sharing 
      data and materials.
EDAT- 2019/09/04 06:00
MHDA- 2020/03/17 06:00
PMCR- 2019/09/03
CRDT- 2019/09/04 06:00
PHST- 2019/05/07 00:00 [received]
PHST- 2019/08/13 00:00 [accepted]
PHST- 2019/09/04 06:00 [entrez]
PHST- 2019/09/04 06:00 [pubmed]
PHST- 2020/03/17 06:00 [medline]
PHST- 2019/09/03 00:00 [pmc-release]
AID - PONE-D-19-12968 [pii]
AID - 10.1371/journal.pone.0221714 [doi]
PST - epublish
SO  - PLoS One. 2019 Sep 3;14(9):e0221714. doi: 10.1371/journal.pone.0221714. 
      eCollection 2019.