PMID- 31480164 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200928 IS - 1011-2367 (Print) IS - 1976-5517 (Electronic) IS - 1011-2367 (Linking) VI - 33 IP - 4 DP - 2020 Apr TI - Dynamic changes and characterization of the protein and carbohydrate fractions of native grass grown in Inner Mongolia during ensiling and the aerobic stage. PG - 556-567 LID - 10.5713/ajas.19.0212 [doi] AB - OBJECTIVE: To improve the utility of native grass resources as feed in China, we investigated the dynamics of protein and carbohydrate fractions among Inner Mongolian native grasses, during ensiling and the aerobic stage, using the Cornell Net Carbohydrate and Protein System. METHODS: Silages were prepared without or with lactic acid bacteria (LAB) inoculant. We analyzed the protein and carbohydrate fractions and fermentation quality of silages at 0, 5, 15, 20, 30, and 60 d of ensiling, and the stability at 0.5, 2, 5, and 10 d during the aerobic stage. RESULTS: Inner Mongolian native grass contained 10.8% crude protein (CP) and 3.6% water-soluble carbohydrates (WSC) on a dry matter basis. During ensiling, pH and CP and WSC content decreased (p<0.05), whereas lactic acid and ammonia nitrogen (N) content increased (p<0.05). Non-protein N (PA) content increased significantly, whereas rapidly degraded true protein (PB1), intermediately degraded true protein (PB2), total carbohydrate (CHO), sugars (CA), starch (CB1), and degradable cell wall carbohydrate (CB2) content decreased during ensiling (p<0.05). At 30 d of ensiling, control and LAB-treated silages were well preserved and had lower pH (<4.2) and ammonia-N content (<0.4 g/kg of fresh matter [FM]) and higher lactic acid content (>1.0% of FM). During the aerobic stage, CP, extract ether, WSC, lactic acid, acetic acid, PB1, PB2, true protein degraded slowly (PB3), CHO, CA, CB1, and CB2 content decreased significantly in all silages, whereas pH, ammonia-N, PA, and bound true protein (PC) content increased significantly. CONCLUSION: Control and LAB-treated silages produced similar results in terms of fermentation quality, aerobic stability, and protein and carbohydrate fractions. Inner Mongolian native grass produced good silage, nutrients were preserved during ensiling and protein and carbohydrate losses largely occurred during the aerobic stage. FAU - Du, Zhumei AU - Du Z AD - Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization of Ministry of Agriculture, Key Laboratory of Grassland Resources, Ministry of Education, P. R. of China, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010019, China. FAU - Risu, Na AU - Risu N AD - The Center of Ecology and Agrometeorology of Inner Mongolia, Hohhot 010019, China. FAU - Gentu, Ge AU - Gentu G AD - Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization of Ministry of Agriculture, Key Laboratory of Grassland Resources, Ministry of Education, P. R. of China, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010019, China. FAU - Jia, Yushan AU - Jia Y AD - Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization of Ministry of Agriculture, Key Laboratory of Grassland Resources, Ministry of Education, P. R. of China, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010019, China. FAU - Cai, Yimin AU - Cai Y AD - Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki 305-8686, Japan. LA - eng GR - 2016YFC0500504/National Natural Science Foundation of China/ GR - 31460638/Ministry of Science and Technology, China/ PT - Journal Article DEP - 20190803 PL - Korea (South) TA - Asian-Australas J Anim Sci JT - Asian-Australasian journal of animal sciences JID - 9884245 PMC - PMC7054601 OTO - NOTNLM OT - Aerobic Stability OT - Cornell Net Carbohydrate and Protein System OT - Inner Mongolian Native Grass OT - Silage Fermentation COIS- CONFLICT OF INTEREST We certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript. EDAT- 2019/09/05 06:00 MHDA- 2019/09/05 06:01 PMCR- 2020/04/01 CRDT- 2019/09/04 06:00 PHST- 2019/03/15 00:00 [received] PHST- 2019/07/12 00:00 [accepted] PHST- 2019/09/05 06:00 [pubmed] PHST- 2019/09/05 06:01 [medline] PHST- 2019/09/04 06:00 [entrez] PHST- 2020/04/01 00:00 [pmc-release] AID - ajas.19.0212 [pii] AID - ajas-19-0212 [pii] AID - 10.5713/ajas.19.0212 [doi] PST - ppublish SO - Asian-Australas J Anim Sci. 2020 Apr;33(4):556-567. doi: 10.5713/ajas.19.0212. Epub 2019 Aug 3.