PMID- 31480464
OWN - NLM
STAT- MEDLINE
DCOM- 20200130
LR  - 20200225
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 24
IP  - 17
DP  - 2019 Aug 31
TI  - Determination of N-Carbamylglutamate in Feeds and Animal Products by High 
      Performance Liquid Chromatography Tandem Mass Spectrometry.
LID - 10.3390/molecules24173172 [doi]
LID - 3172
AB  - N-carbamylglutamate (NCG), a synthetic analogue of N-acetylglutamate, is an 
      activator of blood ammonia conversion and endogenous arginine synthesis. Here, we 
      established an accurate quantitative determination of NCG in feeds, animal 
      tissues, and body fluids using the high performance liquid chromatography tandem 
      mass spectrometry (HPLC-MS/MS). The sample pretreatment procedures included 
      extraction with 0.5% of formic acid in water/methanol (80/20, v/v), and 
      purification using an anionic solid phase extraction cartridge. Satisfactory 
      separation of NCG was achieved in 20 min with the application of an Atlantis T3 
      column, and a confirmative detection of NCG was ensured by multiple reaction 
      monitoring of positive ions. NCG spiked in feeds, tissues, and body fluids were 
      evaluated in regard to linearity, sensitivity, recovery, and repeatability. 
      Recoveries for different sample matrices were in the range of 88.12% to 110.21% 
      with relative standard deviations (RSDs) less than 8.8%. Limits of quantification 
      were within the range of 0.012 to 0.073 mg kg(-1) and 0.047 to 0.077 mug mL(-1) 
      for solid and liquid samples, respectively. This study will provide a solid 
      foundation for the evaluation of availability and metabolic mechanism of NCG in 
      animals.
FAU - Ma, Yonghang
AU  - Ma Y
AD  - State Key Laboratory of Animal Nutrition, College of Animal Science and 
      Technology, China Agricultural University, Beijing 100193, China.
FAU - Zeng, Zhengcheng
AU  - Zeng Z
AD  - State Key Laboratory of Animal Nutrition, College of Animal Science and 
      Technology, China Agricultural University, Beijing 100193, China.
FAU - Kong, Lingchang
AU  - Kong L
AD  - State Key Laboratory of Animal Nutrition, College of Animal Science and 
      Technology, China Agricultural University, Beijing 100193, China.
FAU - Chen, Yuanxin
AU  - Chen Y
AD  - State Key Laboratory of Animal Nutrition, College of Animal Science and 
      Technology, China Agricultural University, Beijing 100193, China.
FAU - He, Pingli
AU  - He P
AD  - State Key Laboratory of Animal Nutrition, College of Animal Science and 
      Technology, China Agricultural University, Beijing 100193, China. 
      hepingli@cau.edu.cn.
LA  - eng
GR  - 2018YFD0500402/The National Key Research and Development Program of China/
PT  - Journal Article
DEP - 20190831
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Glutamates)
RN  - 0 (Solvents)
RN  - 1188-38-1 (N-carbamylglutamate)
SB  - IM
MH  - Animal Feed/*analysis
MH  - Animals
MH  - Cattle
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Glutamates/*analysis/chemistry
MH  - Hydrogen-Ion Concentration
MH  - Limit of Detection
MH  - Solvents
MH  - Swine
MH  - Tandem Mass Spectrometry/*methods
PMC - PMC6749331
OTO - NOTNLM
OT  - HPLC-MS/MS
OT  - N-carbamylglutamate
OT  - animal products
OT  - feeds
OT  - milk
COIS- The authors declare no conflict of interest.
EDAT- 2019/09/05 06:00
MHDA- 2020/01/31 06:00
PMCR- 2019/08/31
CRDT- 2019/09/05 06:00
PHST- 2019/07/18 00:00 [received]
PHST- 2019/08/23 00:00 [revised]
PHST- 2019/08/30 00:00 [accepted]
PHST- 2019/09/05 06:00 [entrez]
PHST- 2019/09/05 06:00 [pubmed]
PHST- 2020/01/31 06:00 [medline]
PHST- 2019/08/31 00:00 [pmc-release]
AID - molecules24173172 [pii]
AID - molecules-24-03172 [pii]
AID - 10.3390/molecules24173172 [doi]
PST - epublish
SO  - Molecules. 2019 Aug 31;24(17):3172. doi: 10.3390/molecules24173172.