PMID- 31481528
OWN - NLM
STAT- MEDLINE
DCOM- 20200928
LR  - 20200928
IS  - 1573-4935 (Electronic)
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 39
IP  - 10
DP  - 2019 Oct 30
TI  - Danggui buxue tang inhibited mesangial cell proliferation and extracellular 
      matrix accumulation through GAS5/NF-kappaB pathway.
LID - BSR20181740 [pii]
LID - 10.1042/BSR20181740 [doi]
AB  - Diabetic nephropathy (DN) is the common complications of diabetes mellitus, but 
      the efficacy of available treatments for the prevention of DN is still 
      unsatisfactory. In the present study, we aimed to explore the effect of Danggui 
      buxue tang (DGT) on the proliferation of high glucose (HG)-induced mesangial 
      cells and accumulation of extracellular matrix in mesangial cells. We found DGT 
      up-regulated the expression of growth arrest specific transcript 5 (GAS5) and IkappaB 
      kinase (IKK) dose-dependently in mouse mesangial cells (SV40 MES-13). We found 
      DGT regulated the expression IKK and the activity of nuclear transcription 
      factor-kappaB (NF-kappaB) via GAS5, and proved that long non-coding RNA (lncRNA) GAS5 was 
      positively related with IKK. And we proved GAS5 regulated the expression of IKK 
      and the activity of NF-kappaB. In addition, DGT inhibited the viability of MES-13 
      cells and extracellular matrix-related proteins (laminin (LN), fibronectin (FN) 
      and collagen IV (Col IV)) via GAS5. Moreover, we proved GAS5 regulated the 
      viability of SV40 MES-13 cells and extracellular matrix-related proteins through 
      NF-kappaB pathway. DGT inhibited the proliferation of mesangial cells and 
      accumulation of extracellular matrix via GAS5/NF-kappaB, therefore, DGT could be an 
      effective treatment for the prevention of DN.
CI  - (c) 2019 The Author(s).
FAU - Zhang, Rui
AU  - Zhang R
AD  - Department of Endocrinology, The Affiliated Hospital of Changchun University of 
      Chinese Medicine, Changchun 130021, Jilin Province, People's Republic of China.
FAU - Han, Xiao
AU  - Han X
AD  - Department of Endocrinology, The Affiliated Hospital of Changchun University of 
      Chinese Medicine, Changchun 130021, Jilin Province, People's Republic of China.
FAU - Huang, Tao
AU  - Huang T
AD  - Department of Emergency Physicians, The Affiliated Hospital of Changchun 
      University of Chinese Medicine, Changchun 130021, Jilin Province, People's 
      Republic of China.
FAU - Wang, Xiuge
AU  - Wang X
AD  - Department of Endocrinology, The Affiliated Hospital of Changchun University of 
      Chinese Medicine, Changchun 130021, Jilin Province, People's Republic of China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (danggui buxue decoction)
RN  - 0 (long non-coding RNA GAS5, mouse)
RN  - EC 2.7.11.10 (I-kappa B Kinase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Cell Proliferation/*drug effects
MH  - Cells, Cultured
MH  - Diabetic Nephropathies/genetics/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Extracellular Matrix/*metabolism
MH  - Extracellular Matrix Proteins/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Glucose/pharmacology
MH  - I-kappa B Kinase/genetics/metabolism
MH  - Male
MH  - Mesangial Cells/cytology/*drug effects/metabolism
MH  - Mice, Inbred C57BL
MH  - NF-kappa B/*metabolism
MH  - RNA Interference
MH  - RNA, Long Noncoding/*genetics
MH  - Signal Transduction/drug effects
PMC - PMC6822488
OTO - NOTNLM
OT  - GAS5
OT  - NF-kappaB
OT  - diabetic nephropathy
OT  - huangtao16125@163.com
OT  - mesangial cell
COIS- The authors declare that there are no competing interests associated with the 
      manuscript.
EDAT- 2019/09/05 06:00
MHDA- 2020/09/29 06:00
PMCR- 2019/10/18
CRDT- 2019/09/05 06:00
PHST- 2018/09/29 00:00 [received]
PHST- 2019/08/01 00:00 [revised]
PHST- 2019/08/28 00:00 [accepted]
PHST- 2019/09/05 06:00 [pubmed]
PHST- 2020/09/29 06:00 [medline]
PHST- 2019/09/05 06:00 [entrez]
PHST- 2019/10/18 00:00 [pmc-release]
AID - BSR20181740 [pii]
AID - 10.1042/BSR20181740 [doi]
PST - ppublish
SO  - Biosci Rep. 2019 Oct 30;39(10):BSR20181740. doi: 10.1042/BSR20181740.