PMID- 31483873 OWN - NLM STAT- MEDLINE DCOM- 20200928 LR - 20201102 IS - 1530-0277 (Electronic) IS - 0145-6008 (Linking) VI - 43 IP - 11 DP - 2019 Nov TI - Reduced Serum Osteocalcin in High-Risk Alcohol Using People Living With HIV Does Not Correlate With Systemic Oxidative Stress or Inflammation: Data From the New Orleans Alcohol Use in HIV Study. PG - 2374-2383 LID - 10.1111/acer.14186 [doi] AB - BACKGROUND: HIV infection is now largely a chronic condition as a result of the success of antiretroviral therapy. However, several comorbidities have emerged in people living with HIV (PLWH), including alcohol use disorders and musculoskeletal disorders. Alcohol use has been associated with lower bone mineral density, alterations to circulating bone turnover markers, and hypocalcemia. The pathophysiological basis of bone loss in the PLWH population is unclear but has been suggested to be linked to oxidative stress and inflammation. To test the hypothesis that PLWH consuming excessive alcohol have altered markers of bone turnover and/or calcium homeostasis in association with oxidative stress, we correlated measurements of alcohol consumption with markers of oxidative stress and inflammation, serum calcium concentrations, and measurements of bone turnover, including c-terminal telopeptide cross-links (CTX-1) and osteocalcin. METHODS: Data were drawn from cross-sectional baseline data from the ongoing New Orleans Alcohol Use in HIV (NOAH) study, comprised of 365 in care PLWH. Alcohol consumption measures (Alcohol Use Disorders Test, 30-day timeline follow-back calendar, and phosphatidylethanol [PEth]) were measured in a subcohort of 40 subjects selected based on highest and lowest PEth measurements. Multivariate linear regression was performed to test the relationships between alcohol consumption and systemic oxidative stress (4-hydroxynonenal; 4-HNE) and inflammation (c-reactive protein; CRP). RESULTS: Serum calcium and CTX-1 did not differ significantly between the high and low-PEth groups. Individuals in the high-PEth group had significantly lower serum osteocalcin (median low-PEth group: 13.42 ng/ml, inter-quartile range [IQR] 9.26 to 14.99 ng/ml; median high-PEth group 7.39 ng/ml, IQR 5.02 to 11.25 ng/ml; p = 0.0005, Wilcoxon rank-sum test). Osteocalcin negatively correlated with PEth (Spearman r = -0.45, p = 0.05) and self-reported measures after adjusting for covariates. Alcohol consumption showed mild, but significant, positive associations with serum 4-HNE, but not with CRP. Osteocalcin did not correlate with either 4-HNE or CRP. CONCLUSIONS: In this subcohort of PLWH, we detected significant associations between at-risk alcohol use and osteocalcin, and at-risk alcohol use and serum 4-HNE, suggesting suppression of bone formation independent of increased systemic oxidative stress with increasing alcohol consumption. CI - (c) 2019 by the Research Society on Alcoholism. FAU - Watt, James AU - Watt J AUID- ORCID: 0000-0002-3256-6522 AD - Comprehensive Alcohol Research Center, Louisiana State University Health Sciences Center, New Orleans, Louisiana. FAU - Schuon, Jonathan AU - Schuon J AD - Department of Orthopedics, Louisiana State University Health Sciences Center, New Orleans, Louisiana. FAU - Davis, Jacob AU - Davis J AD - Department of Orthopedics, Louisiana State University Health Sciences Center, New Orleans, Louisiana. FAU - Ferguson, Tekeda F AU - Ferguson TF AD - Comprehensive Alcohol Research Center, Louisiana State University Health Sciences Center, New Orleans, Louisiana. FAU - Welsh, David A AU - Welsh DA AUID- ORCID: 0000-0002-0008-6161 AD - Comprehensive Alcohol Research Center, Louisiana State University Health Sciences Center, New Orleans, Louisiana. FAU - Molina, Patricia E AU - Molina PE AD - Comprehensive Alcohol Research Center, Louisiana State University Health Sciences Center, New Orleans, Louisiana. AD - Department of Physiology, Louisiana State University Health Sciences Center, New Orleans, Louisiana. FAU - Ronis, Martin J J AU - Ronis MJJ AD - Comprehensive Alcohol Research Center, Louisiana State University Health Sciences Center, New Orleans, Louisiana. LA - eng GR - P60 AA009803/AA/NIAAA NIH HHS/United States GR - F30 AA024680/AA/NIAAA NIH HHS/United States GR - T32 AA007577/AA/NIAAA NIH HHS/United States GR - U54 GM104940/GM/NIGMS NIH HHS/United States GR - R37 AA018282/AA/NIAAA NIH HHS/United States GR - F32 AA024680/AA/NIAAA NIH HHS/United States GR - F32 AA026480/AA/NIAAA NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20191001 PL - England TA - Alcohol Clin Exp Res JT - Alcoholism, clinical and experimental research JID - 7707242 RN - 0 (BGLAP protein, human) RN - 0 (Glycerophospholipids) RN - 0 (phosphatidylethanol) RN - 104982-03-8 (Osteocalcin) RN - SY7Q814VUP (Calcium) SB - IM MH - Alcoholism/blood/*complications/metabolism MH - Calcium/blood/metabolism MH - Cross-Sectional Studies MH - Female MH - Glycerophospholipids/blood MH - HIV Infections/blood/*complications/metabolism MH - Humans MH - Inflammation/blood/*complications/metabolism MH - Male MH - New Orleans MH - Osteocalcin/blood/*deficiency MH - *Oxidative Stress/drug effects PMC - PMC7489311 MID - NIHMS1048824 OTO - NOTNLM OT - Alcohol OT - Bone OT - HIV OT - Oxidative Stress EDAT- 2019/09/05 06:00 MHDA- 2020/09/29 06:00 CRDT- 2019/09/05 06:00 PHST- 2019/07/09 00:00 [received] PHST- 2019/08/22 00:00 [accepted] PHST- 2019/09/05 06:00 [pubmed] PHST- 2020/09/29 06:00 [medline] PHST- 2019/09/05 06:00 [entrez] AID - 10.1111/acer.14186 [doi] PST - ppublish SO - Alcohol Clin Exp Res. 2019 Nov;43(11):2374-2383. doi: 10.1111/acer.14186. Epub 2019 Oct 1.