PMID- 31485672
OWN - NLM
STAT- MEDLINE
DCOM- 20200311
LR  - 20210503
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Linking)
VI  - 55
IP  - 5
DP  - 2019 Nov
TI  - Long non‑coding RNA nuclear paraspeckle assembly transcript 1 interacts with 
      microRNA‑107 to modulate breast cancer growth and metastasis by targeting 
      carnitine palmitoyltransferase‑1.
PG  - 1125-1136
LID - 10.3892/ijo.2019.4869 [doi]
AB  - Previous studies revealed that the long non‑coding RNA nuclear paraspeckle 
      assembly transcript 1 (NEAT1) exhibits abnormal expression in numerous cancer 
      types, including breast cancer (BC); however, the regulatory mechanism of NEAT1 
      in BC remains unclear. In the present study, the effect of NEAT1 on the 
      progression of BC and its regulation mechanism was investigated. The expression 
      levels of NEAT1 and microRNA‑107 (miR‑107) in BC cells were analyzed using the 
      reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). NEAT1 was 
      knocked down in BC cells, and mimics‑miR‑107 or inhibitor‑miR‑107 were 
      transfected into BC cells. Subsequently, cell proliferation, invasion and 
      migration, apoptosis and cell cycle distribution were determined. The regulatory 
      mechanism of NEAT1, miR‑107 and carnitine palmitoyltransferase‑1 (CPT1A) was 
      analyzed using a luciferase reporter assay system, western blotting and RT‑qPCR. 
      NEAT1 expression was increased in BC cells, whereas miR‑107 expression was 
      decreased, compared with normal mammary gland cells. NEAT1 promoted the 
      progression of BC cells through inhibiting apoptosis‑associated genes and 
      promoting cell cycle‑ and invasion‑associated gene expression, whereas miR‑107 
      served the opposite function. Furthermore, NEAT1 promoted the expression of 
      CPT1A, which was mediated by miR‑107. The results of the present study indicate 
      that NEAT1 promotes the expression of CPT1A by inhibiting miR‑107 to improve the 
      progression of BC cells; therefore, NEAT1 is a potential therapeutic target of 
      BC.
FAU - Xiong, Yiquan
AU  - Xiong Y
AD  - Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China.
FAU - Liu, Zeming
AU  - Liu Z
AD  - Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China.
FAU - Li, Zhi
AU  - Li Z
AD  - Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China.
FAU - Wang, Shuntao
AU  - Wang S
AD  - Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China.
FAU - Shen, Na
AU  - Shen N
AD  - Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China.
FAU - Xin, Yue
AU  - Xin Y
AD  - Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China.
FAU - Huang, Tao
AU  - Huang T
AD  - Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190904
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (MIRN107 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NEAT1 long non-coding RNA, human)
RN  - 0 (RNA, Long Noncoding)
RN  - EC 2.3.1.21 (CPT1A protein, human)
RN  - EC 2.3.1.21 (Carnitine O-Palmitoyltransferase)
SB  - IM
MH  - Apoptosis
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Breast Neoplasms/genetics/metabolism/*pathology
MH  - Carnitine O-Palmitoyltransferase/genetics/*metabolism
MH  - Cell Cycle
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Lymphatic Metastasis
MH  - MicroRNAs/*genetics
MH  - RNA, Long Noncoding/*genetics
MH  - Tumor Cells, Cultured
EDAT- 2019/09/06 06:00
MHDA- 2020/03/12 06:00
CRDT- 2019/09/06 06:00
PHST- 2018/02/08 00:00 [received]
PHST- 2019/02/28 00:00 [accepted]
PHST- 2019/09/06 06:00 [pubmed]
PHST- 2020/03/12 06:00 [medline]
PHST- 2019/09/06 06:00 [entrez]
AID - 10.3892/ijo.2019.4869 [doi]
PST - ppublish
SO  - Int J Oncol. 2019 Nov;55(5):1125-1136. doi: 10.3892/ijo.2019.4869. Epub 2019 Sep 
      4.