PMID- 31486507
OWN - NLM
STAT- MEDLINE
DCOM- 20201207
LR  - 20201214
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 16
DP  - 2019 Aug
TI  - Effect of exosome-carried miR-30a on myocardial apoptosis in myocardial 
      ischemia-reperfusion injury rats through regulating autophagy.
PG  - 7066-7072
LID - 18748 [pii]
LID - 10.26355/eurrev_201908_18748 [doi]
AB  - OBJECTIVE: To explore the effect of exosome-carried micro-ribonucleic acid-30a 
      (miR-30a) on myocardial apoptosis in rats with myocardial ischemia-reperfusion 
      injury (MIRI) and its possible regulatory mechanism. MATERIALS AND METHODS: The 
      MIRI rat model was established via ligation of the left anterior descending 
      coronary artery. A total of 30 Sprague-Dawley (SD) rats were randomly divided 
      into Sham group, Model group, and miR-30a inhibitor group. The pathological 
      changes in heart tissues in MIRI rats were detected via hematoxylin-eosin (HE) 
      staining. The levels of serum aspartate aminotransferase (AST) and creatine 
      phosphokinase (CPK) in MIRI rats were detected using the biochemical method. The 
      content of serum malondialdehyde (MDA) and superoxide dismutase (SOD) was 
      detected via enzyme-linked immunosorbent assay (ELISA). Moreover, the apoptosis 
      of heart tissues in MIRI rats was detected via terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) staining. The protein levels 
      of ULK1 and Beclin-1 were detected via Western blotting. RESULTS: Compared with 
      those in the Sham group, the pathological injury of heart tissues was severe, the 
      levels of serum AST and CPK were increased, the content of MDA was decreased, the 
      content of SOD was increased, the apoptotic rate of heart tissues was 
      significantly increased, and the protein levels of ULK1 and Beclin-1 in heart 
      tissues were also significantly increased in Model group. Compared with those in 
      the Model group, the pathological injury of the heart tissues was alleviated, the 
      levels of serum AST and CPK were declined, the content of MDA was increased, the 
      content of SOD was decreased, the apoptotic rate of heart tissues, and the 
      protein levels of ULK1 and Beclin-1 in heart tissues also significantly declined. 
      CONCLUSIONS: The exosome-carried miR-30a inhibitor can suppress the myocardial 
      apoptosis in MIRI rats by reducing autophagy.
FAU - Xu, Y-Q
AU  - Xu YQ
AD  - Department of Cardiovascular Surgery, Weifang People's Hospital, Weifang, China. 
      shuh327@163.com.
FAU - Xu, Y
AU  - Xu Y
FAU - Wang, S-H
AU  - Wang SH
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (MIRN30 microRNA, rat)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Disease Models, Animal
MH  - Exosomes/metabolism
MH  - Male
MH  - MicroRNAs/*metabolism
MH  - Myocardial Reperfusion Injury/*metabolism/pathology
MH  - Myocardium/*metabolism/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2019/09/06 06:00
MHDA- 2020/12/15 06:00
CRDT- 2019/09/06 06:00
PHST- 2019/09/06 06:00 [entrez]
PHST- 2019/09/06 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
AID - 18748 [pii]
AID - 10.26355/eurrev_201908_18748 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Aug;23(16):7066-7072. doi: 
      10.26355/eurrev_201908_18748.