PMID- 31490279
OWN - NLM
STAT- MEDLINE
DCOM- 20200302
LR  - 20200302
IS  - 1536-4798 (Electronic)
IS  - 0277-3740 (Linking)
VI  - 38
IP  - 11
DP  - 2019 Nov
TI  - Comparative Analysis of KGF-2 and bFGF in Prevention of Excessive Wound Healing 
      and Scar Formation in a Corneal Alkali Burn Model.
PG  - 1430-1437
LID - 10.1097/ICO.0000000000002134 [doi]
AB  - PURPOSE: Basic fibroblast growth factor (bFGF) is an effective drug for corneal 
      injury. However, the explicit role of bFGF in corneal scar formation still 
      remains unclear. Keratinocyte growth factor-2 (KGF-2) is associated with the 
      treatment of wound healing. We aimed to compare the efficacy of bFGF and KGF-2 in 
      prevention of excessive wound healing and consequent scar formation in a rat 
      alkali burn model, which provides important clues on the significance of KGF-2 to 
      be developed as a new drug for such injuries. METHODS: The epithelial defect area 
      was evaluated using fluorescein sodium at a concentration of 0.5%. The 
      therapeutic effect of KGF-2 and bFGF on proliferation of rabbit corneal 
      fibroblasts (RCFs) was evaluated by methylthiazoletetrazolium. RCF migration 
      assays were performed with a modified scratch method. Activation of 
      mitogen-activated protein kinase (MAPK) was evaluated by Western blot with 
      specific antibodies. RESULTS: All corneal wounds treated with KGF-2 were found 
      closed within 7 days; however, the wounds treated with bFGF or phosphate buffer 
      saline (PBS) required 14 days to close. RCFs treated with KGF-2 or bFGF showed 
      similar dose-dependent proliferation. The KGF-2 group significantly promoted cell 
      migration compared with the bFGF group. The KGF-2 group showed less expression of 
      alpha-smooth muscle actin (SMA) and numbers of myofibroblasts compared with the bFGF 
      group. Our findings suggested identification of cascade reaction of extracellular 
      regulated protein kinases (ERK)1/2 and p38 signals in KGF-2- and bFGF-induced 
      proliferation and migration of RCFs. In addition, KGF-2 showed stronger effects 
      during ERK1/2 and p38 phosphorylation in methylthiazoletetrazolium proliferation 
      assay and scratch migration assay. CONCLUSIONS: KGF-2 exhibited better effects 
      than bFGF in reepithelialization, acceleration of migration, and reduction of 
      scar formation, which has potential to become a new drug to cure corneal injury.
FAU - Cai, JianQiu
AU  - Cai J
AD  - Department of Ophthalmology, The Affiliated Second Hospital of Wenzhou Medical 
      University, Wenzhou, China.
AD  - Department of Pharmacy, School of Pharmacy, Wenzhou Medical University, Chashan 
      University Park, Wenzhou, China.
FAU - Zhou, Qingde
AU  - Zhou Q
AD  - Department of Pharmacy, School of Pharmacy, Wenzhou Medical University, Chashan 
      University Park, Wenzhou, China.
FAU - Wang, Zhitao
AU  - Wang Z
AD  - Department of Pharmacy, School of Pharmacy, Wenzhou Medical University, Chashan 
      University Park, Wenzhou, China.
FAU - Guo, Ruide
AU  - Guo R
AD  - Department of Ophthalmology, the Affiliated First Hospital of Wenzhou Medical 
      University, Wenzhou, China.
FAU - Yang, Rongshuai
AU  - Yang R
AD  - Department of Pharmacy, School of Pharmacy, Wenzhou Medical University, Chashan 
      University Park, Wenzhou, China.
FAU - Yang, Xuanxin
AU  - Yang X
AD  - Department of Pharmacy, School of Pharmacy, Wenzhou Medical University, Chashan 
      University Park, Wenzhou, China.
FAU - Li, Wenqing
AU  - Li W
AD  - Department of Pharmacy, School of Pharmacy, Wenzhou Medical University, Chashan 
      University Park, Wenzhou, China.
FAU - Ahmad, Naveed
AU  - Ahmad N
AD  - Department of molecular biology, College of Life Sciences, Jilin Agricultural 
      University, Changchun, China.
FAU - Chen, Qi
AU  - Chen Q
AD  - Department of Pharmacy, School of Pharmacy, Wenzhou Medical University, Chashan 
      University Park, Wenzhou, China.
AD  - Department of Ophthalmology, School of Ophthalmology and Optometry, Wenzhou 
      Medical University, Wenzhou, Zhejiang, China.
FAU - Hui, Qi
AU  - Hui Q
AD  - Department of Ophthalmology, The Affiliated Second Hospital of Wenzhou Medical 
      University, Wenzhou, China.
AD  - Department of Pharmacy, School of Pharmacy, Wenzhou Medical University, Chashan 
      University Park, Wenzhou, China.
FAU - Wang, Xiaojie
AU  - Wang X
AD  - Department of Ophthalmology, The Affiliated Second Hospital of Wenzhou Medical 
      University, Wenzhou, China.
AD  - Department of Pharmacy, School of Pharmacy, Wenzhou Medical University, Chashan 
      University Park, Wenzhou, China.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Cornea
JT  - Cornea
JID - 8216186
RN  - 0 (Alkalies)
RN  - 0 (Fibroblast Growth Factor 10)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Alkalies/toxicity
MH  - Animals
MH  - Burns, Chemical/complications/*drug therapy/pathology
MH  - Cell Movement/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Cornea/drug effects/pathology
MH  - Corneal Injuries/complications/*drug therapy/pathology
MH  - Disease Models, Animal
MH  - Eye Burns/complications/*drug therapy/pathology
MH  - Fibroblast Growth Factor 10/*pharmacology
MH  - Fibroblast Growth Factor 2/*pharmacology
MH  - Keloid/etiology/pathology/*prevention & control
MH  - Microscopy, Acoustic
MH  - Rats
MH  - Wound Healing/*drug effects
EDAT- 2019/09/07 06:00
MHDA- 2020/03/03 06:00
CRDT- 2019/09/07 06:00
PHST- 2019/09/07 06:00 [pubmed]
PHST- 2020/03/03 06:00 [medline]
PHST- 2019/09/07 06:00 [entrez]
AID - 10.1097/ICO.0000000000002134 [doi]
PST - ppublish
SO  - Cornea. 2019 Nov;38(11):1430-1437. doi: 10.1097/ICO.0000000000002134.