PMID- 31490706
OWN - NLM
STAT- MEDLINE
DCOM- 20210512
LR  - 20210512
IS  - 1460-2202 (Electronic)
IS  - 0271-3683 (Linking)
VI  - 45
IP  - 4
DP  - 2020 Apr
TI  - Alteration of Tear Cytokine Expressions in Primary Acquired Nasolacrimal Duct 
      Obstruction - Potential Insights into the Etiopathogenesis.
PG  - 435-439
LID - 10.1080/02713683.2019.1665186 [doi]
AB  - Purpose: To investigate the presence and level of 35 distinct cytokines in the 
      tear fluid obtained from patients with primary acquired nasolacrimal duct 
      obstruction (PANDO) and compare it with controls in an effort to understand the 
      disease etiopathogenesis.Methods: Standard protocols were used for collecting 
      tears from 60 eyes (20 diseased eyes and 20 healthy fellow eyes of unilateral 
      PANDO, 20 control eyes of healthy subjects). A total of 35 analytes involved in 
      inflammation, angiogenesis and wound healing were assessed by multiplex ELISA. 
      Alterations in the tear levels of cytokines in PANDO and their comparison with 
      the levels in the non-diseased fellow eye and healthy volunteers were noted. 
      STRING analysis was used to assess the involved biological pathways of the 
      altered cytokines. Linear mixed effect model was used for statistical analysis. A 
      P value of <0.05 was considered significant.Results: There was significant 
      upregulation of 10 pro-inflammatory cytokines in tears from diseased eyes of 
      PANDO patients in comparison with the non-diseased controls and include matrix 
      metalloproteinase 9 (MMP 9), serpin E1, Interleukin-6 (IL-6), hepatocyte growth 
      factor (HGF), vascular endothelial growth factor-A and R2 (VEGF-A, VEGF R2), 
      platelet-endothelial cell adhesion molecule (PECAM-1), c-reactive protein (CRP), 
      chemokine ligand 2 (CCL2) and platelet-derived growth factor- AA (PDGF-AA). 
      Amongst the anti-inflammatory cytokines, three were significantly upregulated in 
      diseased eyes of PANDO patients in comparison with the non-diseased controls and 
      include granulocyte-colony stimulating factor (G-CSF), retinol binding protein 4 
      (RBP4) and tissue inhibitor of metalloproteinases -1 (TIMP-1). There were no 
      significant differences between the control eyes of the diseased patient and 
      control eyes of healthy subjects. Based on the significantly altered cytokines, 
      string analysis revealed that the biological pathways involved in the 
      etiopathogenesis of PANDO include inflammation, angiogenesis, negative regulation 
      of apoptosis, cellular proliferation and hormonal regulation.Conclusions: In 
      cases of PANDO, dysregulation of certain cytokines was disease specific. 
      Biological pathways reflect a possible link and interaction between the 
      inflammatory cytokines with vasculature and hormonal microenvironments of the 
      lacrimal drainage system, which in a way is bringing three promising candidates 
      in the PANDO etiopathogenesis on a common ground.
FAU - Ali, Mohammad Javed
AU  - Ali MJ
AD  - Govindram Seksaria Institute of Dacryology, L.V. Prasad Eye Institute, Hyderabad, 
      India.
FAU - Patnaik, Satish
AU  - Patnaik S
AD  - Prof. Brien Holden Eye Research Center, L.V. Prasad Eye Institute, Hyderabad, 
      India.
FAU - Kelkar, Natasha
AU  - Kelkar N
AD  - Prof. Brien Holden Eye Research Center, L.V. Prasad Eye Institute, Hyderabad, 
      India.
FAU - Ali, Mohammad Hasnat
AU  - Ali MH
AD  - Prof. Brien Holden Eye Research Center, L.V. Prasad Eye Institute, Hyderabad, 
      India.
FAU - Kaur, Inderjeet
AU  - Kaur I
AD  - Prof. Brien Holden Eye Research Center, L.V. Prasad Eye Institute, Hyderabad, 
      India.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191004
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
SB  - IM
MH  - Biomarkers/metabolism
MH  - Case-Control Studies
MH  - Cytokines/*biosynthesis
MH  - Female
MH  - Humans
MH  - Lacrimal Duct Obstruction/etiology/*metabolism
MH  - Male
MH  - Nasolacrimal Duct/*metabolism
MH  - Tears/*metabolism
OTO - NOTNLM
OT  - PANDO
OT  - cytokine
OT  - lacrimal
OT  - lacrimal sac
OT  - nasolacrimal duct
OT  - tear
EDAT- 2019/09/07 06:00
MHDA- 2021/05/13 06:00
CRDT- 2019/09/07 06:00
PHST- 2019/09/07 06:00 [pubmed]
PHST- 2021/05/13 06:00 [medline]
PHST- 2019/09/07 06:00 [entrez]
AID - 10.1080/02713683.2019.1665186 [doi]
PST - ppublish
SO  - Curr Eye Res. 2020 Apr;45(4):435-439. doi: 10.1080/02713683.2019.1665186. Epub 
      2019 Oct 4.