PMID- 31492038 OWN - NLM STAT- MEDLINE DCOM- 20200727 LR - 20221122 IS - 2218-273X (Electronic) IS - 2218-273X (Linking) VI - 9 IP - 9 DP - 2019 Sep 5 TI - Differential Sympathetic Activation of Adipose Tissues by Brain-Derived Neurotrophic Factor. LID - 10.3390/biom9090452 [doi] LID - 452 AB - Centrally administered brain-derived neurotrophic factor (BDNF) decreases body adiposity beyond what can be accounted for by decreased food intake, implying enhanced lipid metabolism by BDNF. Consistent with this notion, intracerebroventricular (icv) injection of BDNF in rats increased the expression of lipolytic enzymes in white adipose tissues (WAT) and increased circulating concentrations of lipolytic products without changing the levels of adrenal gland hormones. This suggests that central BDNF-induced lipid mobilization is likely due to sympathetic neural activation, rather than activation of the adrenocortical or adrenomedullary system. We hypothesized that BDNF activated sympathetic innervation of adipose tissues to regulate lipolysis. Rats with unilateral denervation of interscapular brown adipose tissue (BAT) and different WAT depots received icv injections of saline or BDNF. Both intact and denervated adipose tissues were exposed to the same circulating factors, but denervated adipose tissues did not receive neural signals. Norepinephrine (NE) turnover (NETO) of BAT and WAT was assessed as a measure of sympathetic activity. Findings revealed that central BDNF treatment induced a change in NETO in some but not all the adipose tissues tested. Specifically, greater NETO rates were found in BAT and gonadal epididymal WAT (EWAT), but not in inguinal WAT (IWAT) or retroperitoneal WAT (RWAT), of BDNF-treated rats compared to saline-treated rats. Furthermore, intact innervation was necessary for BDNF-induced NETO in BAT and EWAT. In addition, BDNF increased the expression of lipolytic enzymes in both intact and denervated EWAT and IWAT, suggesting that BDNF-induced WAT lipolysis was independent of intact innervation. To summarize, centrally administered BDNF selectively provoked sympathetic drives to BAT and EWAT that was dependent on intact innervation, while BDNF also increased lipolysis in a manner independent of intact innervation. FAU - Zhu, Qi AU - Zhu Q AD - Department of Biology, Miami University, Oxford, OH 45056, USA. FAU - Liu, Xian AU - Liu X AD - Department of Biology, Miami University, Oxford, OH 45056, USA. FAU - Glazier, Bradley J AU - Glazier BJ AD - Department of Biology, Miami University, Oxford, OH 45056, USA. FAU - Krolick, Kristen N AU - Krolick KN AD - Department of Biology, Miami University, Oxford, OH 45056, USA. FAU - Yang, Shangyuwen AU - Yang S AD - Department of Biology, Miami University, Oxford, OH 45056, USA. FAU - He, Jingyan AU - He J AD - Department of Biology, Miami University, Oxford, OH 45056, USA. FAU - Lo, Chunmin C AU - Lo CC AD - Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, Diabetes Institute, Ohio University, Athens, OH 45701, USA. loc1@ohio.edu. FAU - Shi, Haifei AU - Shi H AD - Department of Biology, Miami University, Oxford, OH 45056, USA. Haifei.Shi@miamioh.edu. LA - eng GR - K01 DK083550/DK/NIDDK NIH HHS/United States GR - R15 DK118611/DK/NIDDK NIH HHS/United States GR - HHMI/Howard Hughes Medical Institute/United States GR - NIH DK090823/NH/NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20190905 PL - Switzerland TA - Biomolecules JT - Biomolecules JID - 101596414 RN - 0 (Bdnf protein, rat) RN - 0 (Brain-Derived Neurotrophic Factor) SB - IM MH - Adipose Tissue/*metabolism MH - Animals MH - Brain-Derived Neurotrophic Factor/administration & dosage/*metabolism MH - Lipolysis MH - Male MH - Rats MH - Rats, Long-Evans MH - Sympathetic Nervous System/*metabolism PMC - PMC6769916 OTO - NOTNLM OT - adrenal hormones OT - brown adipose tissue OT - denervation OT - energy homeostasis OT - lipolysis OT - sympathetic activity OT - white adipose tissue COIS- The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results. EDAT- 2019/09/08 06:00 MHDA- 2020/07/28 06:00 PMCR- 2019/09/01 CRDT- 2019/09/08 06:00 PHST- 2019/06/30 00:00 [received] PHST- 2019/08/17 00:00 [revised] PHST- 2019/09/02 00:00 [accepted] PHST- 2019/09/08 06:00 [entrez] PHST- 2019/09/08 06:00 [pubmed] PHST- 2020/07/28 06:00 [medline] PHST- 2019/09/01 00:00 [pmc-release] AID - biom9090452 [pii] AID - biomolecules-09-00452 [pii] AID - 10.3390/biom9090452 [doi] PST - epublish SO - Biomolecules. 2019 Sep 5;9(9):452. doi: 10.3390/biom9090452.