PMID- 3149468
OWN - NLM
STAT- MEDLINE
DCOM- 19890518
LR  - 20190903
IS  - 0006-2928 (Print)
IS  - 0006-2928 (Linking)
VI  - 26
IP  - 9-10
DP  - 1988 Oct
TI  - Genetic control of ganglioside biosynthesis in mice.
PG  - 631-44
AB  - The enzymatic basis for the differences in hepatic ganglioside patterns in the 
      mouse strains C57Bl/6 and Swiss White (SW) was investigated. SW has a 
      "Swiss-type" ganglioside profile, expressing GM1- and GD1a- in addition to GM2- 
      as major hepatic gangliosides, whereas C57Bl/6 shows a "GM2-type" profile, 
      expressing only GM2- as the major hepatic ganglioside. The enzyme 
      UDP-galactose:GM2 ganglioside galactosyltransferase (GM2-GalT), which catalyzes 
      the synthesis of GM1 ganglioside, showed a four- to fivefold elevation in intact 
      and solubilized liver Golgi membrane fractions of the SW strain compared to 
      C57Bl/6. Crosses between C57Bl/6 and SW produced an F1 generation with a hepatic 
      ganglioside and enzymatic phenotype intermediate between those of the two 
      parental strains. All three genotypic groups show two forms of the Golgi 
      apparatus enzyme with isoelectric points of 6.5-6.8 and 8.3-9.0. The simplest 
      mode of action of genes which control the enzymatic phenotype that would be 
      consistent with these findings are one or two structural genes or one or two 
      cis-regulatory genes affecting the rate of enzyme synthesis.
FAU - Sokoloff, D
AU  - Sokoloff D
AD  - Department of Biology, McGill University, Montreal, Quebec, Canada.
FAU - Hechtman, P
AU  - Hechtman P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Genet
JT  - Biochemical genetics
JID - 0126611
RN  - 0 (Gangliosides)
RN  - 0 (Isoenzymes)
RN  - EC 2.4.1.- (Galactosyltransferases)
RN  - EC 2.4.1.62 (Ganglioside Galactosyltransferase)
SB  - IM
MH  - Animals
MH  - Crosses, Genetic
MH  - Galactosyltransferases/*genetics/metabolism
MH  - Ganglioside Galactosyltransferase
MH  - Gangliosides/*biosynthesis
MH  - *Genes
MH  - Genes, Regulator
MH  - Golgi Apparatus/enzymology
MH  - Isoenzymes/*genetics/metabolism
MH  - Liver/*enzymology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Species Specificity
EDAT- 1988/10/01 00:00
MHDA- 1988/10/01 00:01
CRDT- 1988/10/01 00:00
PHST- 1988/10/01 00:00 [pubmed]
PHST- 1988/10/01 00:01 [medline]
PHST- 1988/10/01 00:00 [entrez]
AID - 10.1007/BF02399607 [doi]
PST - ppublish
SO  - Biochem Genet. 1988 Oct;26(9-10):631-44. doi: 10.1007/BF02399607.