PMID- 31496042
OWN - NLM
STAT- MEDLINE
DCOM- 20210609
LR  - 20210609
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 108
IP  - 1
DP  - 2020 Jan
TI  - Asiaticoside loading into polylactic-co-glycolic acid electrospun nanofibers 
      attenuates host inflammatory response and promotes M2 macrophage polarization.
PG  - 69-80
LID - 10.1002/jbm.a.36793 [doi]
AB  - Polylactic-co-glycolic acid (PLGA) is one of the most promising synthetic 
      materials for tissue engineering due to its excellent biocompatibility, good 
      mechanical properties, and tunable biodegradation time. However, the accumulation 
      of PLGA degradative products could cause significant host inflammatory response, 
      a microenvironment favoring tissue fibrosis that is mainly mediated by M1 subtype 
      macrophage. Drug loading is an emerging technology to modify electrospun 
      nanofibers, and asiaticoside (AS) was demonstrated as an anti-inflammatory drug. 
      This study investigated the potential effect of AS incorporating into PLGA 
      electrospun nanofibers on modulating host inflammatory response. The results 
      showed that AS co-electrospun with PLGA nanofibers could significantly reduce the 
      infiltration of inflammatory cells at the implantation site as opposed to the 
      site of regular PLGA nanofibers. In particular, immunohistochemistry demonstrated 
      decreased M1 macrophage infiltration whereas increased M2 macrophage infiltration 
      in the implantation site of AS-PLGA nanofibers when compared to the PLGA 
      implantation site. In vitro study also revealed that culture of human fibroblasts 
      on PLGA nanofibers resulted in significantly enhanced gene expression of 
      inflammatory cytokines when compared to non-seeded fibroblasts, but these genes 
      were significantly downregulated when seeded on AS-PLGA. Furthermore, culture of 
      macrophage on AS-PLGA led to upregulated M2 marker gene expression and 
      downregulated M1 marker gene expression. Collectively, these results indicate 
      that, AS might be an ideal drug for loading into electrospun polymer nanofibers 
      and thus favoring for tissue regeneration via mediating macrophage polarization.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Huang, Jia
AU  - Huang J
AD  - Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's 
      Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key 
      Laboratory of Tissue Engineering Research, Shanghai, China.
FAU - Zhou, Xiaobo
AU  - Zhou X
AD  - Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's 
      Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key 
      Laboratory of Tissue Engineering Research, Shanghai, China.
FAU - Shen, Yanbing
AU  - Shen Y
AD  - College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 
      Shanghai, China.
FAU - Li, Haibo
AU  - Li H
AD  - Xiangya Medical College of Central South University, Changsha, Hunan, China.
FAU - Zhou, Guangdong
AU  - Zhou G
AD  - Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's 
      Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key 
      Laboratory of Tissue Engineering Research, Shanghai, China.
AD  - National Tissue Engineering Center of China, Shanghai, China.
FAU - Zhang, Wenjie
AU  - Zhang W
AD  - Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's 
      Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key 
      Laboratory of Tissue Engineering Research, Shanghai, China.
AD  - National Tissue Engineering Center of China, Shanghai, China.
FAU - Zhang, Yanzhong
AU  - Zhang Y
AD  - College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 
      Shanghai, China.
AD  - State Key Laboratory for Modification of Chemical Nanofibers and Polymer 
      Materials, Donghua University, Shanghai, China.
AD  - Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, 
      Donghua University, Shanghai, China.
FAU - Liu, Wei
AU  - Liu W
AD  - Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's 
      Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key 
      Laboratory of Tissue Engineering Research, Shanghai, China.
AD  - National Tissue Engineering Center of China, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191012
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Triterpenes)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - PKO39VY215 (asiaticoside)
SB  - IM
MH  - Animals
MH  - *Cell Polarity/drug effects
MH  - Dermis/cytology
MH  - Female
MH  - Fibroblasts/drug effects/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Inflammation/*pathology
MH  - Macrophages/drug effects/*pathology
MH  - Male
MH  - Nanofibers/*chemistry
MH  - Phenotype
MH  - Polylactic Acid-Polyglycolic Acid Copolymer/*chemistry
MH  - Rats, Sprague-Dawley
MH  - *Tissue Engineering
MH  - Triterpenes/*pharmacology
OTO - NOTNLM
OT  - Asiaticoside
OT  - PLGA nanofibers
OT  - anti-inflammatory
OT  - electrospinning
OT  - macrophage polarization
EDAT- 2019/09/10 06:00
MHDA- 2021/06/10 06:00
CRDT- 2019/09/10 06:00
PHST- 2019/05/28 00:00 [received]
PHST- 2019/08/17 00:00 [revised]
PHST- 2019/09/04 00:00 [accepted]
PHST- 2019/09/10 06:00 [pubmed]
PHST- 2021/06/10 06:00 [medline]
PHST- 2019/09/10 06:00 [entrez]
AID - 10.1002/jbm.a.36793 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2020 Jan;108(1):69-80. doi: 10.1002/jbm.a.36793. Epub 2019 
      Oct 12.