PMID- 31497518
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220410
IS  - 2228-7523 (Print)
IS  - 2228-7531 (Electronic)
IS  - 2228-7523 (Linking)
VI  - 9
IP  - 3
DP  - 2019 Jun
TI  - The Basic Study of the Mechanism of Propofol-Related Infusion Syndrome Using a 
      Murine Skeletal Muscle Injury Model.
PG  - e89417
LID - 10.5812/aapm.89417 [doi]
LID - e89417
AB  - BACKGROUND: The pathophysiological mechanism of propofol-related infusion 
      syndrome (PRIS) is believed to be due to the injury to the mitochondrial electron 
      transport chain and the resultant metabolic disorders that are caused by both 
      propofol agents and the lipid solvent. However, the mechanisms and causative 
      factors of PRIS have not been fully elucidated. OBJECTIVES: The aim of this study 
      was to evaluate the possibility of a research model using the culture of 
      differentiated C2C12 cells for fundamental research of PRIS. METHODS: First, 
      differentiated C2C12 cells were cultured accompanied by several concentrations of 
      chemical reagents of 2,6-diisopropylphenol (2,6 DIP) or dimethyl sulfoxide (DMSO) 
      for 60 hours and the cell death rate was examined by trypan blue staining. 
      Second, The cells were incubated with a commercially available propofol reagent 
      or lipid reagent for 48 hours. The supernatant fluid of the cell culture medium 
      was gathered and the numbers of floating cells were measured by cell counter. To 
      investigate the mitochondrial disorder by the propofol preparation, JC-1, an 
      experiment using fluorescent reagent, was performed for the 48 hours with 100 
      microg/mL propofol incubation. RESULTS: The rate of cell death was increased with 
      elevating concentrations both of chemical reagents of 2,6 DIP group and dimethyl 
      sulfoxide group. The rates of cell death were significantly higher in the 2,6 DIP 
      group than DMSO group. The numbers of floating cells were increased with 
      elevating concentrations both commercially available propofol reagent and lipid 
      reagent groups. The decreased red/green fluorescence ratio by JC-1 staining in 
      the propofol 100microg/mL group proved an attenuated mitochondrial membrane 
      potential. CONCLUSIONS: The dose-dependent cell damage induced by the propofol 
      reagents and a lipid solvent may provide a proposed model as a basic experimental 
      model for further investigations into PRIS.
FAU - Murakami, Yuryo
AU  - Murakami Y
AD  - Department of Anesthesiology and Pain Medicine, Hyogo College of Medicine, 
      Nishinomiya, Japan.
FAU - Ueki, Ryusuke
AU  - Ueki R
AD  - Department of Anesthesiology and Pain Medicine, Hyogo College of Medicine, 
      Nishinomiya, Japan.
FAU - Tachikawa, Taihei
AU  - Tachikawa T
AD  - Department of Anesthesiology, Meiwa Hospital, Nishinomiya, Japan.
FAU - Hirose, Munetaka
AU  - Hirose M
AD  - Department of Anesthesiology and Pain Medicine, Hyogo College of Medicine, 
      Nishinomiya, Japan.
LA  - eng
PT  - Journal Article
DEP - 20190423
PL  - Netherlands
TA  - Anesth Pain Med
JT  - Anesthesiology and pain medicine
JID - 101585412
PMC - PMC6712282
OTO - NOTNLM
OT  - C2C12 Cell
OT  - Mitochondria
OT  - Propofol-Related Infusion Syndrome (PRIS)
OT  - Rhabdomyolysis
OT  - Skeletal Muscle
EDAT- 2019/09/10 06:00
MHDA- 2019/09/10 06:01
PMCR- 2019/04/23
CRDT- 2019/09/10 06:00
PHST- 2019/01/17 00:00 [received]
PHST- 2019/03/05 00:00 [revised]
PHST- 2019/04/07 00:00 [accepted]
PHST- 2019/09/10 06:00 [entrez]
PHST- 2019/09/10 06:00 [pubmed]
PHST- 2019/09/10 06:01 [medline]
PHST- 2019/04/23 00:00 [pmc-release]
AID - 10.5812/aapm.89417 [doi]
PST - epublish
SO  - Anesth Pain Med. 2019 Apr 23;9(3):e89417. doi: 10.5812/aapm.89417. eCollection 
      2019 Jun.