PMID- 31498530
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230201
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 44
IP  - 1
DP  - 2020 Jan
TI  - Bradykinin receptor-1 activation induces inflammation and increases the 
      permeability of human brain microvascular endothelial cells.
PG  - 343-351
LID - 10.1002/cbin.11232 [doi]
AB  - Neuroinflammatory disorders such as Alzheimer's and Parkinson's diseases are 
      characterised by chronic inflammation and loss of vascular integrity. Bradykinin 
      1 receptor (B1R) activation has been implicated in many neuroinflammatory 
      diseases, but the contribution of B1R to inflammation and vascular breakdown is 
      yet to be determined. As a result, the present study evaluated the effect of B1R 
      stimulation using Des-Arg-9-BK on the cytokine profile and junctional properties 
      of human cerebral microvascular endothelial cells (hCMVECs). Results showed that 
      stimulation of B1R receptors increased secretion of pro-inflammatory cytokines, 
      interleukin-6 (IL-6), IL-8, intracellular adhesion molecule-1 (ICAM-1), vascular 
      cell adhesion molecule-1 (VCAM-1) and monocyte chemoattractant protein-1 (MCP-1), 
      but decreased the expression of vascular endothelial growth factor (VEGF), a 
      cytokine and growth factor required for maintenance of the vasculature. B1R 
      stimulation also resulted in the loss of occludin expression at tight junctions 
      with no change in VE-cadherin expression. There was also a significant increase 
      in permeability to Evans blue albumin, suggesting an increase of vascular 
      permeability. Taken together, these results suggest that B1R activation that 
      occurs in neuroinflammatory diseases may contribute to both the inflammation and 
      loss of blood-brain barrier integrity that is characteristic of these diseases.
CI  - (c) 2019 International Federation for Cell Biology.
FAU - Mugisho, Odunayo O
AU  - Mugisho OO
AD  - Department of Anatomy and Medical Imaging and Centre for Brain Research, School 
      of Medical Sciences, Faculty of Medical and Health Sciences, University of 
      Auckland, Private Bag 92019, Auckland, 1142, New Zealand.
AD  - Department of Ophthalmology, Buchanan Ocular Therapeutics Unit, New Zealand 
      National Eye Centre, University of Auckland, Private Bag 92019, Auckland, 1142, 
      New Zealand.
FAU - Robilliard, Laverne D
AU  - Robilliard LD
AD  - Department of Molecular Medicine and Pathology and Centre for Brain Research, 
      School of Medical Sciences, Faculty of Medical and Health Sciences, University of 
      Auckland, Private Bag 92019, Auckland, 1142, New Zealand.
FAU - Nicholson, Louise F B
AU  - Nicholson LFB
AD  - Department of Anatomy and Medical Imaging and Centre for Brain Research, School 
      of Medical Sciences, Faculty of Medical and Health Sciences, University of 
      Auckland, Private Bag 92019, Auckland, 1142, New Zealand.
FAU - Graham, E Scott
AU  - Graham ES
AD  - Department of Molecular Medicine and Pathology and Centre for Brain Research, 
      School of Medical Sciences, Faculty of Medical and Health Sciences, University of 
      Auckland, Private Bag 92019, Auckland, 1142, New Zealand.
FAU - O'Carroll, Simon J
AU  - O'Carroll SJ
AD  - Department of Anatomy and Medical Imaging and Centre for Brain Research, School 
      of Medical Sciences, Faculty of Medical and Health Sciences, University of 
      Auckland, Private Bag 92019, Auckland, 1142, New Zealand.
LA  - eng
PT  - Journal Article
DEP - 20190923
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
SB  - IM
OTO - NOTNLM
OT  - blood-brain barrier
OT  - bradykinin
OT  - endothelial cells
OT  - inflammation
OT  - neurodegenerative diseases
OT  - pro-inflammatory cytokines
EDAT- 2019/09/10 06:00
MHDA- 2019/09/10 06:01
CRDT- 2019/09/10 06:00
PHST- 2018/10/09 00:00 [received]
PHST- 2019/08/31 00:00 [accepted]
PHST- 2019/09/10 06:00 [pubmed]
PHST- 2019/09/10 06:01 [medline]
PHST- 2019/09/10 06:00 [entrez]
AID - 10.1002/cbin.11232 [doi]
PST - ppublish
SO  - Cell Biol Int. 2020 Jan;44(1):343-351. doi: 10.1002/cbin.11232. Epub 2019 Sep 23.