PMID- 31502009
OWN - NLM
STAT- MEDLINE
DCOM- 20200203
LR  - 20200225
IS  - 1573-4838 (Electronic)
IS  - 0957-4530 (Linking)
VI  - 30
IP  - 9
DP  - 2019 Sep 9
TI  - An injectable thermosensitive hydrogel for sustained release of apelin-13 to 
      enhance flap survival in rat random skin flap.
PG  - 106
LID - 10.1007/s10856-019-6306-y [doi]
AB  - With the advantage of handy process, random pattern skin flaps are generally 
      applied in limb reconstruction and wound repair. Apelin-13 is a discovered 
      endogenous peptide, that has been shown to have potent multiple biological 
      functions. Recently, thermosensitive gel-forming systems have gained increasing 
      attention as wound dressings due to their advantages. In the present study, an 
      apelin-13-loaded chitosan (CH)/beta-sodium glycerophosphate (beta-GP) hydrogel was 
      developed for promoting random skin flap survival. Random skin flaps were created 
      in 60 rats after which the animals were categorized to a control hydrogel group 
      and an apelin-13 hydrogel group. The water content of the flap as well as the 
      survival area were then measured 7 days post-surgery. Hematoxylin and eosin 
      staining was used to evaluate the flap angiogenesis. Cell differentiation 34 
      (CD34) and vascular endothelial growth factor (VEGF) levels were detected by 
      immunohistochemistry and Western blotting. Tumor necrosis factor-alpha (TNF-alpha) and 
      interleukin-6 (IL-6) were assessed by enzyme linked immunosorbent assays 
      (ELISAs). Oxidative stress was estimated via the activity of tissue 
      malondialdehyde (MDA) and superoxide dismutase (SOD). Our results showed that 
      CH/beta-GP/apelin-13 hydrogel could not only reduce the tissue edema, but also 
      improve the survival area of flap. CH/beta-GP/apelin-13 hydrogel also upregulated 
      levels of VEGF protein and increased mean vessel densities. Furthermore, 
      CH/beta-GP/apelin-13 hydrogel was shown to significantly inhibit the expression of 
      TNF-alpha and IL-6, along with increasing the activity of SOD and suppressing the MDA 
      content. Taken together, these results indicate that this CH/beta-GP/apelin-13 
      hydrogel may be a potential therapeutic way for random pattern skin flap.
FAU - Zheng, Wenhao
AU  - Zheng W
AD  - Department of Orthopaedic Surgery, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, 325000, Wenzhou, China.
FAU - Wang, Jinwu
AU  - Wang J
AD  - Department of Orthopaedic Surgery, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, 325000, Wenzhou, China.
FAU - Xie, Linzhen
AU  - Xie L
AD  - Department of Orthopaedic Surgery, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, 325000, Wenzhou, China.
FAU - Xie, Huanguang
AU  - Xie H
AD  - Department of Orthopaedic Surgery, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, 325000, Wenzhou, China.
FAU - Chen, Chunhui
AU  - Chen C
AD  - Department of Orthopaedic Surgery, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, 325000, Wenzhou, China.
FAU - Zhang, Chuanxu
AU  - Zhang C
AD  - Department of Orthopaedic Surgery, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, 325000, Wenzhou, China.
FAU - Lin, Dingsheng
AU  - Lin D
AD  - Department of Orthopaedic Surgery, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, 325000, Wenzhou, China.
FAU - Cai, Leyi
AU  - Cai L
AD  - Department of Orthopaedic Surgery, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, 325000, Wenzhou, China. 
      caileyi@wmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190909
PL  - United States
TA  - J Mater Sci Mater Med
JT  - Journal of materials science. Materials in medicine
JID - 9013087
RN  - 0 (Delayed-Action Preparations)
RN  - 0 (Hydrogels)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (apelin-13 peptide)
RN  - 4Y8F71G49Q (Malondialdehyde)
SB  - IM
MH  - Animals
MH  - Body Temperature/physiology
MH  - Delayed-Action Preparations/administration & dosage/pharmacokinetics
MH  - Drug Evaluation, Preclinical
MH  - Graft Survival/*drug effects
MH  - Hydrogels/administration & dosage/pharmacokinetics
MH  - Intercellular Signaling Peptides and Proteins/*administration & 
      dosage/*pharmacokinetics
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Necrosis/pathology/prevention & control
MH  - Oxidative Stress/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Skin/*drug effects/metabolism/pathology
MH  - Skin Physiological Phenomena/drug effects
MH  - Skin Transplantation/*methods
MH  - Surgical Flaps/physiology/transplantation
MH  - *Temperature
EDAT- 2019/09/11 06:00
MHDA- 2020/02/06 06:00
CRDT- 2019/09/11 06:00
PHST- 2019/04/05 00:00 [received]
PHST- 2019/08/29 00:00 [accepted]
PHST- 2019/09/11 06:00 [entrez]
PHST- 2019/09/11 06:00 [pubmed]
PHST- 2020/02/06 06:00 [medline]
AID - 10.1007/s10856-019-6306-y [pii]
AID - 10.1007/s10856-019-6306-y [doi]
PST - epublish
SO  - J Mater Sci Mater Med. 2019 Sep 9;30(9):106. doi: 10.1007/s10856-019-6306-y.