PMID- 31505067
OWN - NLM
STAT- MEDLINE
DCOM- 20200306
LR  - 20200306
IS  - 1439-0507 (Electronic)
IS  - 0933-7407 (Linking)
VI  - 62
IP  - 12
DP  - 2019 Dec
TI  - Different DNA methylation profile is demonstrated in paracoccidioidomycosis 
      patients without oral lesions.
PG  - 1133-1139
LID - 10.1111/myc.13000 [doi]
AB  - BACKGROUND: Paracoccidioidomycosis (PCM) is a neglected fungal infection with a 
      high impact on the quality of life of the affected patients. The disease presents 
      primary pulmonary involvement and systemic dissemination may occur. About 50% of 
      the cases show oral involvement, and the factors that lead to this manifestation 
      are not clear. OBJECTIVES: The aim of this study was to evaluate the DNA 
      methylation profile in PCM patients with oral lesions. MATERIAL AND METHODS: 
      Genomic DNA was extracted from whole blood of eighteen PCM patients, being ten 
      with oral lesions and eight with no oral lesion. Analysis of methylation profile 
      was performed using the technique of methylation-sensitive arbitrarily primed PCR 
      (MS-AP-PCR). The sequences of recombinant plasmids obtained were evaluated 
      according to parameters that define a CpG island, as well as their relative 
      position in the known human genome genes and/or CpG islands. RESULTS: After DNA 
      amplification, three different expressed bands were observed between the two 
      groups, being found in the samples of patients with no oral manifestations. The 
      cloned fragment in the plasmid showed similarity with a DNA sequence present in 
      chromosome 20, next to the YTHDF1 gene. Other bands showed homology with intronic 
      region in the genes RBPMS2 and DPH6 and no CpG island was identified. 
      CONCLUSIONS: DNA methylation was found in PCM patients with no oral lesion 
      affecting the YTHDF1 gene. Further studies are necessary to elucidate to role of 
      YTHDF1 gene in the oral PCM manifestations.
CI  - (c) 2019 Blackwell Verlag GmbH.
FAU - Singi, Paola
AU  - Singi P
AD  - Department of Clinic and Surgery, School of Dentistry, Federal University of 
      Alfenas, Alfenas, MG, Brazil.
FAU - Rocha, Raissa P
AU  - Rocha RP
AD  - Graduate Program in Microbiology, Federal University of Minas Gerais, Belo 
      Horizonte, MG, Brazil.
FAU - de Carli, Marina L
AU  - de Carli ML
AD  - Department of Clinic and Surgery, School of Dentistry, Federal University of 
      Alfenas, Alfenas, MG, Brazil.
FAU - Hanemann, Joao Adolfo C
AU  - Hanemann JAC
AD  - Department of Clinic and Surgery, School of Dentistry, Federal University of 
      Alfenas, Alfenas, MG, Brazil.
FAU - Pereira, Alessandro A C
AU  - Pereira AAC
AD  - Department of Pathology and Parasitology, Institute of Biomedical Sciences, 
      Federal University of Alfenas, Alfenas, MG, Brazil.
FAU - Coelho, Luiz Felipe L
AU  - Coelho LFL
AD  - Department of Microbiology and Immunology, Institute of Biomedical Sciences, 
      Federal University of Alfenas, Alfenas, MG, Brazil.
FAU - Malaquias, Luiz Cosme C
AU  - Malaquias LCC
AUID- ORCID: 0000-0002-4920-072X
AD  - Department of Microbiology and Immunology, Institute of Biomedical Sciences, 
      Federal University of Alfenas, Alfenas, MG, Brazil.
LA  - eng
GR  - 001/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/
PT  - Journal Article
DEP - 20191001
PL  - Germany
TA  - Mycoses
JT  - Mycoses
JID - 8805008
SB  - IM
MH  - Adult
MH  - *DNA Methylation
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Mouth/*microbiology/*pathology
MH  - Paracoccidioides
MH  - Paracoccidioidomycosis/*genetics
MH  - Sequence Analysis, DNA
OTO - NOTNLM
OT  - DNA methylation
OT  - oral manifestations
OT  - paracoccidioidomycosis
EDAT- 2019/09/11 06:00
MHDA- 2020/03/07 06:00
CRDT- 2019/09/11 06:00
PHST- 2019/06/10 00:00 [received]
PHST- 2019/09/04 00:00 [revised]
PHST- 2019/09/04 00:00 [accepted]
PHST- 2019/09/11 06:00 [pubmed]
PHST- 2020/03/07 06:00 [medline]
PHST- 2019/09/11 06:00 [entrez]
AID - 10.1111/myc.13000 [doi]
PST - ppublish
SO  - Mycoses. 2019 Dec;62(12):1133-1139. doi: 10.1111/myc.13000. Epub 2019 Oct 1.