PMID- 31511141
OWN - NLM
STAT- MEDLINE
DCOM- 20200810
LR  - 20200810
IS  - 1448-5990 (Electronic)
IS  - 1031-3613 (Linking)
VI  - 31
IP  - 11
DP  - 2019 Oct
TI  - Arcuate nucleus kisspeptin response to increased nutrition in rams.
PG  - 1682-1691
LID - 10.1071/RD19063 [doi]
AB  - Rams respond to acute nutritional supplementation by increasing the frequency of 
      gonadotrophin-releasing hormone (GnRH) pulses. Kisspeptin neurons may mediate the 
      effect of environmental cues on GnRH secretion, so we tested whether the ram 
      response to nutrition involves activation of kisspeptin neurons in the arcuate 
      nucleus (ARC), namely kisspeptin, neurokin B, dynorphin (KNDy) neurons. Rams were 
      given extra lupin grain with their normal ration. Blood was sampled before 
      feeding, and continued until animals were killed for collection of brain tissue 
      at 2 or 11h after supplementation. In supplemented rams, LH pulse frequency 
      increased after feeding, whereas control animals showed no change. Within the 
      caudal ARC, there were more kisspeptin neurons in supplemented rams than in 
      controls and a higher proportion of kisspeptin cells coexpressed Fos, regardless 
      of the time the rams were killed. There were more Fos cells in the mid-ARC and 
      mid-dorsomedial hypothalamus of the supplemented compared with control rams. No 
      effect of nutrition was found on kisspeptin expression in the rostral or mid-ARC, 
      or on GnRH expression in the preoptic area. Kisspeptin neurons in the caudal ARC 
      appear to mediate the increase in GnRH and LH production due to acute nutritional 
      supplementation, supporting the hypothesised role of the KNDy neurons as the 
      pulse generator for GnRH.
FAU - Rietema, S E
AU  - Rietema SE
AD  - School of Agriculture and Environment, The University of Western Australia, 35 
      Stirling Highway, Perth, WA 6009, Australia.
FAU - Hawken, P A R
AU  - Hawken PAR
AD  - School of Agriculture and Environment, The University of Western Australia, 35 
      Stirling Highway, Perth, WA 6009, Australia.
FAU - Scott, C J
AU  - Scott CJ
AD  - School of Biomedical Sciences, Charles Sturt University, Boorooma Street, Wagga 
      Wagga, NSW 2678, Australia.
FAU - Lehman, M N
AU  - Lehman MN
AD  - Brain Health Research Institute and Department of Biological Sciences, Kent State 
      University, PO Box 5190, Kent, OH 44242-0001, USA.
FAU - Martin, G B
AU  - Martin GB
AD  - School of Agriculture and Environment, The University of Western Australia, 35 
      Stirling Highway, Perth, WA 6009, Australia.
FAU - Smith, J T
AU  - Smith JT
AD  - The School of Human Sciences, The University of Western Australia, 35 Stirling 
      Highway, Perth, WA 6009, Australia; and Corresponding author. Email: 
      jeremy.smith@uwa.edu.au.
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 0 (Kisspeptins)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - *Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Arcuate Nucleus of Hypothalamus/*metabolism
MH  - Energy Metabolism/physiology
MH  - Gonadotropin-Releasing Hormone/metabolism
MH  - Kisspeptins/*metabolism
MH  - Luteinizing Hormone/metabolism
MH  - Male
MH  - Neurons/metabolism
MH  - Overnutrition/*metabolism/veterinary
MH  - Sheep, Domestic/*physiology
EDAT- 2019/09/13 06:00
MHDA- 2020/08/11 06:00
CRDT- 2019/09/13 06:00
PHST- 2019/02/18 00:00 [received]
PHST- 2019/06/16 00:00 [accepted]
PHST- 2019/09/13 06:00 [pubmed]
PHST- 2020/08/11 06:00 [medline]
PHST- 2019/09/13 06:00 [entrez]
AID - RD19063 [pii]
AID - 10.1071/RD19063 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 2019 Oct;31(11):1682-1691. doi: 10.1071/RD19063.