PMID- 31512599
OWN - NLM
STAT- MEDLINE
DCOM- 20200127
LR  - 20200127
IS  - 2212-554X (Electronic)
IS  - 2212-5531 (Linking)
VI  - 8
IP  - 3
DP  - 2019 Jul-Sep
TI  - Expression of mycolic acid in response to stress and association with 
      differential clinical manifestations of tuberculosis.
PG  - 237-243
LID - 10.4103/ijmy.ijmy_69_19 [doi]
AB  - BACKGROUND: Extrapulmonary tuberculosis (EPTB), accounting for 10%-20% of all 
      cases of tuberculosis (TB), is known to be determined by host immunity. However, 
      the contribution of bacterial factors to the development of EPTB has not been 
      studied extensively. Mycolic acids are predominant lipids constituting the cell 
      wall of Mycobacterium tuberculosis, and keto-mycolic acid is involved in the 
      synthesis of foamy macrophages that facilitate persistence of mycobacteria. 
      Hence, the present study was performed to gain an insight into variable 
      expression of mycolic acids in clinical isolates of M. tuberculosis under stress. 
      METHODS: Pansusceptible clinical isolates of M. tuberculosis from patients with 
      lymph node TB (LNTB) (n = 10) and pulmonary TB (PTB) (n = 10) were subjected to 
      sodium dodecyl sulfate (SDS) stress, and the expression of mycolic acid and its 
      biosynthetic genes was compared. Any bias arising due to the genotype of the 
      clinical isolates was ruled out by performing single-nucleotide polymorphism 
      cluster grouping (SCG), wherein no significant difference was observed between 
      the SCG of LNTB or PTB isolates. RESULTS: The expression of alpha-mycolic acid during 
      the exposure to SDS was high in 7/10 (70%) LNTB and 6/10 (60%) PTB isolates. 
      Methoxy mycolic acid showed an increased expression in 7/10 (70%) LNTB isolates 
      and 4/10 (40%) PTB isolates. Increased expression of keto-mycolic acid on 
      exposure with SDS was observed in 8/10 (80%) M. tuberculosis LNTB and 3/10 (30%) 
      PTB isolates. Similarly, the mycolic acid synthesis gene, fas, was upregulated 
      more in LNTB isolates than PTB isolates in vitro and ex vivo. SCG 3a was the most 
      common SCG observed in 40% (8/20) of the isolates, followed by SCG 3b in 30% 
      (6/20) of the isolates. There was no significant difference between the SCG of 
      LNTB or PTB isolates. CONCLUSION: The higher expression of keto-mycolic acid in 
      LNTB as against PTB isolates may indicate better survival in LNTB isolates in the 
      presence of stress.
FAU - Sharma, Naresh Kumar
AU  - Sharma NK
AD  - Department of Microbiology, Vallabhbhai Patel Chest Institute, University of 
      Delhi, Delhi, India.
FAU - Rathor, Nisha
AU  - Rathor N
AD  - Department of Microbiology, Vallabhbhai Patel Chest Institute, University of 
      Delhi, Delhi, India.
FAU - Sinha, Rajesh
AU  - Sinha R
AD  - Department of Microbiology, Vallabhbhai Patel Chest Institute, University of 
      Delhi, Delhi, India.
FAU - Gupta, Shraddha
AU  - Gupta S
AD  - Department of Microbiology, Vallabhbhai Patel Chest Institute, University of 
      Delhi, Delhi, India.
FAU - Tyagi, Gaurav
AU  - Tyagi G
AD  - Department of Microbiology, Vallabhbhai Patel Chest Institute, University of 
      Delhi, Delhi, India.
FAU - Garima, Kushal
AU  - Garima K
AD  - Department of Microbiology, Vallabhbhai Patel Chest Institute, University of 
      Delhi, Delhi, India.
FAU - Pathak, Rakesh
AU  - Pathak R
AD  - Department of Microbiology, Vallabhbhai Patel Chest Institute, University of 
      Delhi, Delhi, India.
FAU - Singh, Pooja
AU  - Singh P
AD  - Department of Microbiology, Vallabhbhai Patel Chest Institute, University of 
      Delhi, Delhi, India.
FAU - Jain, Ashima
AU  - Jain A
AD  - Department of Microbiology, Vallabhbhai Patel Chest Institute, University of 
      Delhi, Delhi, India.
FAU - Bose, Mridula
AU  - Bose M
AD  - Department of Microbiology, Vallabhbhai Patel Chest Institute, University of 
      Delhi, Delhi, India.
FAU - Varma-Basil, Mandira
AU  - Varma-Basil M
AD  - Department of Microbiology, Vallabhbhai Patel Chest Institute, University of 
      Delhi, Delhi, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Int J Mycobacteriol
JT  - International journal of mycobacteriology
JID - 101615660
RN  - 0 (Bacterial Proteins)
RN  - 0 (Mycolic Acids)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
SB  - IM
MH  - Bacterial Proteins/genetics
MH  - Gene Expression/*drug effects
MH  - Genotype
MH  - Humans
MH  - Mycobacterium tuberculosis/*drug effects/*genetics
MH  - Mycolic Acids/*metabolism
MH  - Polymorphism, Single Nucleotide
MH  - Sodium Dodecyl Sulfate/pharmacology
MH  - Stress, Physiological
MH  - THP-1 Cells
MH  - Tuberculosis, Lymph Node/microbiology
MH  - Tuberculosis, Pulmonary/microbiology
OTO - NOTNLM
OT  - Lymph node tuberculosis
OT  - mycolic acid
OT  - pulmonary tuberculosis
OT  - surface stress
COIS- None
EDAT- 2019/09/13 06:00
MHDA- 2020/01/28 06:00
CRDT- 2019/09/13 06:00
PHST- 2019/09/13 06:00 [entrez]
PHST- 2019/09/13 06:00 [pubmed]
PHST- 2020/01/28 06:00 [medline]
AID - IntJMycobacteriol_2019_8_3_237_266495 [pii]
AID - 10.4103/ijmy.ijmy_69_19 [doi]
PST - ppublish
SO  - Int J Mycobacteriol. 2019 Jul-Sep;8(3):237-243. doi: 10.4103/ijmy.ijmy_69_19.