PMID- 31513744
OWN - NLM
STAT- MEDLINE
DCOM- 20200511
LR  - 20231113
IS  - 1535-4989 (Electronic)
IS  - 1044-1549 (Print)
IS  - 1044-1549 (Linking)
VI  - 62
IP  - 1
DP  - 2020 Jan
TI  - Embedding of Precision-Cut Lung Slices in Engineered Hydrogel Biomaterials 
      Supports Extended Ex Vivo Culture.
PG  - 14-22
LID - 10.1165/rcmb.2019-0232MA [doi]
AB  - Maintaining the three-dimensional architecture and cellular complexity of lung 
      tissue ex vivo can enable elucidation of the cellular and molecular pathways 
      underlying chronic pulmonary diseases. Precision-cut lung slices (PCLS) are one 
      human-lung model with the potential to support critical mechanistic studies and 
      early drug discovery. However, many studies report short culture times of 7-10 
      days. Here, we systematically evaluated poly(ethylene glycol)-based hydrogel 
      platforms for the encapsulation of PCLS. We demonstrated the ability to support 
      ex vivo culture of embedded PCLS for at least 21 days compared with control PCLS 
      floating in media. These customized hydrogels maintained PCLS architecture (no 
      difference), viability (4.7-fold increase, P < 0.0001), and cellular phenotype as 
      measured by SFTPC (1.8-fold increase, P < 0.0001) and vimentin expression (no 
      change) compared with nonencapsulated controls. Collectively, these results 
      demonstrate that hydrogel biomaterials support the extended culture times 
      required to study chronic pulmonary diseases ex vivo using PCLS technology.
FAU - Bailey, Kolene E
AU  - Bailey KE
AD  - Division of Pulmonary Sciences and Critical Care Medicine, Department of Medicine 
      and.
FAU - Pino, Christopher
AU  - Pino C
AD  - Department of Bioengineering, University of Colorado, Aurora, Colorado.
FAU - Lennon, Mallory L
AU  - Lennon ML
AD  - Department of Bioengineering, University of Colorado, Aurora, Colorado.
FAU - Lyons, Anne
AU  - Lyons A
AD  - Department of Bioengineering, University of Colorado, Aurora, Colorado.
FAU - Jacot, Jeffrey G
AU  - Jacot JG
AUID- ORCID: 0000-0002-1272-5055
AD  - Department of Bioengineering, University of Colorado, Aurora, Colorado.
FAU - Lammers, Steven R
AU  - Lammers SR
AD  - Department of Bioengineering, University of Colorado, Aurora, Colorado.
FAU - Konigshoff, Melanie
AU  - Konigshoff M
AD  - Division of Pulmonary Sciences and Critical Care Medicine, Department of Medicine 
      and.
FAU - Magin, Chelsea M
AU  - Magin CM
AUID- ORCID: 0000-0002-6988-8584
AD  - Division of Pulmonary Sciences and Critical Care Medicine, Department of Medicine 
      and.
AD  - Department of Bioengineering, University of Colorado, Aurora, Colorado.
LA  - eng
GR  - T32 HL007085/HL/NHLBI NIH HHS/United States
GR  - UL1 TR002535/TR/NCATS NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Am J Respir Cell Mol Biol
JT  - American journal of respiratory cell and molecular biology
JID - 8917225
RN  - 0 (Biocompatible Materials)
RN  - 0 (Hydrogels)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/*administration & dosage
MH  - Humans
MH  - Hydrogels/*administration & dosage
MH  - Lung/*pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Organ Culture Techniques/*methods
MH  - Pulmonary Disease, Chronic Obstructive/pathology
PMC - PMC6938134
OTO - NOTNLM
OT  - biomaterial
OT  - hydrogel
OT  - precision-cut lung slice
OT  - pulmonary disease modeling
OT  - three-dimensional printing
EDAT- 2019/09/13 06:00
MHDA- 2020/05/12 06:00
PMCR- 2021/01/01
CRDT- 2019/09/13 06:00
PHST- 2019/09/13 06:00 [pubmed]
PHST- 2020/05/12 06:00 [medline]
PHST- 2019/09/13 06:00 [entrez]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.1165/rcmb.2019-0232MA [doi]
PST - ppublish
SO  - Am J Respir Cell Mol Biol. 2020 Jan;62(1):14-22. doi: 10.1165/rcmb.2019-0232MA.