PMID- 31513788
OWN - NLM
STAT- MEDLINE
DCOM- 20200708
LR  - 20200708
IS  - 1873-7064 (Electronic)
IS  - 0028-3908 (Linking)
VI  - 160
DP  - 2019 Dec 1
TI  - Role of astrocytic GABAergic system on inflammatory cytokine-induced anxiety-like 
      behavior.
PG  - 107776
LID - S0028-3908(18)30687-7 [pii]
LID - 10.1016/j.neuropharm.2019.107776 [doi]
AB  - Recent studies have shown that not only neurons but astrocytes contain a 
      considerable amount of gamma-aminobutyric acid (GABA), which can be released and 
      activate the receptors responsive to GABA. The purpose of this study is to test 
      whether gliotransmitters from astrocytes may play a role in etiology of anxiety 
      symptoms. Intracerebroventricular (i.c.v.) infusion of interleukin-1beta (IL-1beta), 
      one of potent inflammatory cytokines, induced anxiety-like behaviors and 
      activated the glial fibrillary acidic protein (GFAP) in the paraventricular 
      nucleus (PVN) of the hypothalamus. Pretreatment with astrocytes toxin, 
      l-alpha-aminoadipate (L-AAA) reduced anxiety-like behaviors and the GFAP expression 
      in the PVN. Intraparaventricular nucleus (iPVN) infusion of IL-1beta produced 
      markedly anxiety-like behaviors and increased release of GABA from astrocytes. 
      However, treatment of glial cell inhibitor, L-AAA or blocker of Bestrophin-1 
      (Best1), 5-Nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) markedly inactivated 
      astrocytes and also reduced the anxiety-like behaviors. Treatment of L-AAA or 
      NPPB decreased IL-1beta-induced gliotransmitter GABA release measured by in vivo 
      microdialysis. These results suggest that selective inhibition of astrocytes or 
      astocytic GABA release in the PVN may serve as an effective therapeutic strategy 
      for treating anxiety and affective disorders.
CI  - Copyright (c) 2019. Published by Elsevier Ltd.
FAU - Shim, Hyun Soo
AU  - Shim HS
AD  - Department of Physiology, College of Medicine, Kyung Hee University, 1 
      Hoegi-dong, Dongys0daemun-gu, Seoul, 02447, South Korea; Center for Neuroscience, 
      Brain Science Institute of Science and Technology (KIST), Seoul, 02792, South 
      Korea.
FAU - Park, Hyun Jung
AU  - Park HJ
AD  - Department of Physiology, College of Medicine, Kyung Hee University, 1 
      Hoegi-dong, Dongys0daemun-gu, Seoul, 02447, South Korea; Department of Food 
      Science and Biotechnology, Kyonggi University, 154-42, Gwanggyosan-ro, 
      Youngtong-gu, Suwon, Gyeonggi, 16227, South Korea.
FAU - Woo, Junsung
AU  - Woo J
AD  - Center for Neuroscience, Brain Science Institute of Science and Technology 
      (KIST), Seoul, 02792, South Korea.
FAU - Lee, C Justin
AU  - Lee CJ
AD  - Center for Neuroscience, Brain Science Institute of Science and Technology 
      (KIST), Seoul, 02792, South Korea.
FAU - Shim, Insop
AU  - Shim I
AD  - Department of Physiology, College of Medicine, Kyung Hee University, 1 
      Hoegi-dong, Dongys0daemun-gu, Seoul, 02447, South Korea. Electronic address: 
      ishim@khu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190909
PL  - England
TA  - Neuropharmacology
JT  - Neuropharmacology
JID - 0236217
RN  - 0 (Cytokines)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (IL1B protein, rat)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Nitrobenzoates)
RN  - 3A35O9G3YZ (5-nitro-2-(3-phenylpropylamino)benzoic acid)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
SB  - IM
MH  - Animals
MH  - Anxiety/*chemically induced/drug therapy
MH  - Astrocytes/*metabolism
MH  - Behavior, Animal
MH  - Cytokines/metabolism
MH  - GABAergic Neurons/*metabolism
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Inflammation/metabolism
MH  - Infusions, Intraventricular
MH  - Interleukin-1beta/*administration & dosage/adverse effects
MH  - Male
MH  - Nitrobenzoates/therapeutic use
MH  - Paraventricular Hypothalamic Nucleus/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - gamma-Aminobutyric Acid/adverse effects/metabolism
OTO - NOTNLM
OT  - 5-Nitro-2-(3-phenylpropylamino) benzoicacid (NPPB)
OT  - Anxiety
OT  - Glial fibrillary acidic protein (GFAP)
OT  - Interleukin-1beta (IL-1beta)
OT  - l-alpha-aminoadipate (L-AAA)
OT  - gamma-aminobutyric acid (GABA)
EDAT- 2019/09/13 06:00
MHDA- 2020/07/09 06:00
CRDT- 2019/09/13 06:00
PHST- 2018/09/19 00:00 [received]
PHST- 2019/09/07 00:00 [revised]
PHST- 2019/09/08 00:00 [accepted]
PHST- 2019/09/13 06:00 [pubmed]
PHST- 2020/07/09 06:00 [medline]
PHST- 2019/09/13 06:00 [entrez]
AID - S0028-3908(18)30687-7 [pii]
AID - 10.1016/j.neuropharm.2019.107776 [doi]
PST - ppublish
SO  - Neuropharmacology. 2019 Dec 1;160:107776. doi: 10.1016/j.neuropharm.2019.107776. 
      Epub 2019 Sep 9.