PMID- 31514828
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20190930
LR  - 20190930
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 206
DP  - 2020 Jan 1
TI  - Use of ammonium sulfite as a post-column derivatization reagent for rapid 
      detection and quantification of aldehydes by LC-MS.
PG  - 120172
LID - S0039-9140(19)30804-5 [pii]
LID - 10.1016/j.talanta.2019.120172 [doi]
AB  - Derivatization is frequently used in liquid chromatography-mass spectrometry 
      (LC-MS) to improve the ionization characteristics of analytes, and facilitate 
      their structural elucidation and quantification. However, the off-line 
      derivatization process usually requires more analysis time. Therefore, the 
      present work develops a novel LC/post-column derivatization (PCD)/MS (LC-PCD-MS) 
      method for rapid detection of aldehydes in complex samples. The PCD method is 
      based on a bisulfite addition reaction with aldehydes that can be easily 
      conducted by mixing the LC elute with an (NH(4))(2)SO(3) solution via a tee. The 
      resulting derivatives readily produce diagnostic HSO(3)(-) ions under collision 
      induced dissociation (CID) in the negative ion mode of MS, even at an extremely 
      low CID energy such as 1 eV, which can significantly improve the detection 
      selectivity of aldehydes and reduce the occurrence of false positives. The limits 
      of detection (LODs) of hexanal and heptanal were 3 nM and 2 nM, respectively. 
      Moreover, good linearity in the concentration range of 0.01-1 muM for hexanal and 
      heptanal was obtained with the determination coefficient (R(2)) being greater 
      than 0.996 and the intra- and inter-day relative standard deviations (RSDs) were 
      lower than 13.5% and 17.9%, respectively, indicating the acceptable 
      reproducibility of the method. The LC-PCD-MS method was successfully applied for 
      the rapid quantification of aldehydes in human urine and solvent extracts of 
      traditional Chinese medicines (TCMs). Furthermore, a precursor ion scan strategy 
      based on the diagnostic HSO(3)(-) ion was also developed and applied for the 
      preliminary screening of aldehydes in complex samples of cinnamon and lemongrass.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Hu, Yu-Ning
AU  - Hu YN
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China.
FAU - Chen, Di
AU  - Chen D
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China.
FAU - Zhang, Tian-Yi
AU  - Zhang TY
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China.
FAU - Ding, Jun
AU  - Ding J
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China.
FAU - Feng, Yu-Qi
AU  - Feng YQ
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China. 
      Electronic address: yqfeng@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190727
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
SB  - IM
OTO - NOTNLM
OT  - Aldehyde
OT  - Bisulfite
OT  - LC-MS
OT  - Post column derivatization
EDAT- 2019/09/14 06:00
MHDA- 2019/09/14 06:01
CRDT- 2019/09/14 06:00
PHST- 2019/06/16 00:00 [received]
PHST- 2019/07/22 00:00 [revised]
PHST- 2019/07/23 00:00 [accepted]
PHST- 2019/09/14 06:00 [entrez]
PHST- 2019/09/14 06:00 [pubmed]
PHST- 2019/09/14 06:01 [medline]
AID - S0039-9140(19)30804-5 [pii]
AID - 10.1016/j.talanta.2019.120172 [doi]
PST - ppublish
SO  - Talanta. 2020 Jan 1;206:120172. doi: 10.1016/j.talanta.2019.120172. Epub 2019 Jul 
      27.