PMID- 31515080
OWN - NLM
STAT- MEDLINE
DCOM- 20200424
LR  - 20200424
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Linking)
VI  - 224
IP  - 6
DP  - 2019 Nov
TI  - Diagnostic and prognostic roles of peripheral blood Toll-like receptor-4 and 
      stanniocalcin-1 genes expression in acute lung injury.
PG  - 734-738
LID - S0171-2985(19)30147-0 [pii]
LID - 10.1016/j.imbio.2019.09.003 [doi]
AB  - Acute lung injury (ALI) is an acute inflammatory disorder. Toll-like receptor-4 
      (TLR-4) and Stanniocalcin -1 (STC-1) had roles in lung endothelial protection. 
      This study aims to assess TLR-4 and SCT-1 genes expressions in peripheral blood 
      of ALI patients. Total RNA was extracted from peripheral blood of 48 subjects (20 
      healthy controls, 28 ALI patients) and expressions of genes were assessed by 
      real-Time qRT-PCR. The expression levels of TLR-4 and SCT-1 genes were 
      significantly lower in ALI patients compared to controls (P < 0.0001). After 10 
      days, the expression levels of TLR-4 and SCT-1 were increased compared to their 
      baseline levels (p = 0.012 and 0.024, respectively). SCT-1 has 92.9% sensitivity 
      and 100% specificity in ALI detection. SCT-1 gene expression was negatively 
      correlated with severity score (r= -0.54, p = 0.003). The mortality pattern was 
      higher in ALI patients with lower TLR-4 gene expression (p = 0.014). In 
      conclusion, the peripheral blood expressions of TLR-4 and STC-1 genes were 
      decreased in ALI patients. Both genes expressions were increased with patients' 
      recovery. SCT-1 had higher sensitivity and specificity in ALI diagnosis. The 
      peripheral blood expressions of SCT-1 and TLR-4 genes seem to be diagnostic and 
      prognostic markers in ALI.
CI  - Copyright (c) 2019 Elsevier GmbH. All rights reserved.
FAU - Esawy, Marwa M
AU  - Esawy MM
AD  - Clinical Pathology Department, Faculty of Human Medicine, Zagazig University, 
      Egypt. Electronic address: dr.marwaesawy@ymail.com.
FAU - Shabana, Marwa A
AU  - Shabana MA
AD  - Clinical Pathology Department, Faculty of Human Medicine, Zagazig University, 
      Egypt.
FAU - Baioumy, Shereen A
AU  - Baioumy SA
AD  - Microbiology and Immunology Department, Faculty of Human Medicine, Zagazig 
      University, Egypt.
FAU - Ismail, Nagwan A
AU  - Ismail NA
AD  - Chest Department, Faculty of Human Medicine, Zagazig University, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20190904
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Glycoproteins)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 76687-96-2 (teleocalcin)
SB  - IM
MH  - Acute Lung Injury/diagnosis/*genetics
MH  - Adult
MH  - Female
MH  - Gene Expression
MH  - Glycoproteins/*genetics
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - Severity of Illness Index
MH  - Toll-Like Receptor 4/*genetics
OTO - NOTNLM
OT  - Acute lung injury
OT  - Stanniocalcin
OT  - Toll-like receptor
EDAT- 2019/09/14 06:00
MHDA- 2020/04/25 06:00
CRDT- 2019/09/14 06:00
PHST- 2019/04/30 00:00 [received]
PHST- 2019/08/16 00:00 [revised]
PHST- 2019/09/03 00:00 [accepted]
PHST- 2019/09/14 06:00 [pubmed]
PHST- 2020/04/25 06:00 [medline]
PHST- 2019/09/14 06:00 [entrez]
AID - S0171-2985(19)30147-0 [pii]
AID - 10.1016/j.imbio.2019.09.003 [doi]
PST - ppublish
SO  - Immunobiology. 2019 Nov;224(6):734-738. doi: 10.1016/j.imbio.2019.09.003. Epub 
      2019 Sep 4.