PMID- 31515517
OWN - NLM
STAT- MEDLINE
DCOM- 20201021
LR  - 20210110
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Sep 12
TI  - A novel kit for early diagnosis of Alzheimer's disease using a fluorescent 
      nanoparticle imaging.
PG  - 13184
LID - 10.1038/s41598-019-49711-y [doi]
LID - 13184
AB  - Alzheimer's disease (AD) is a progressive neurodegenerative disease and chronic 
      illness with long preclinical phases and a long clinical duration. Until 
      recently, a lack of potential therapeutic agents against AD was the primary focus 
      of research, which resulted in less effort directed towards developing useful 
      diagnostic approaches. In this study, we developed a WO2002/088706 kit that is 
      composed of fluorescent nanoparticles for the early detection of AD. We provided 
      a fluorescent nanoparticle for detecting markers and a kit for the early 
      diagnosis of AD. The kit consists of a probe molecule comprising an 
      oligonucleotide capable of detecting one or more AD-specific microRNAs (miRNAs) 
      and biomarkers related to AD. Through screening, we selected miR-106b, miR-146b, 
      miR-181a, miR-200a, miR-34a, miR-124b, miR-153, miR-155, Abeta(1-42) monomer (mAbeta), 
      Abeta(1-42) oligomer (oAbeta), UCHL1, NLRP3, Tau, STAT3, SORL1, Clusterin, APOE3, 
      APOE4, Nogo-A, IL-13, and Visfatin to serve as AD- and inflammation-related 
      markers. For detection of kit-binding properties, we checked the expression 
      levels of amyloid beta (Abeta), tau protein, and inflammatory mediators in 
      APP/PS/ApoE knockdown (KD) mice and a control group using co-localisation 
      analysis conducted with a confocal microscope. Using a similar approach, we 
      checked the expression levels of miRNAs in HT22 cells. Finally, we used the 
      plasma from AD patients to confirm that our fluorescent nanoparticles and the 
      WO2002/088706 kit will provide a possible early diagnosis to serve as an AD 
      detector that can be further improved for future studies on targeting AD.
FAU - Park, Jun Sung
AU  - Park JS
AD  - Division of Life Science and Applied Life Science (BK21 plus), College of Natural 
      Sciences, Gyeongsang National University (GNU), Jinju, 52802, Republic of Korea.
FAU - Kim, Sang Tae
AU  - Kim ST
AD  - Department of Neurology, Seoul National University Bundang Hospital, Seongnam-si, 
      Gyeonggi-do, 13605, Republic of Korea.
FAU - Kim, Sang Yun
AU  - Kim SY
AD  - Department of Neurology, Seoul National University Bundang Hospital, Seongnam-si, 
      Gyeonggi-do, 13605, Republic of Korea.
FAU - Jo, Min Gi
AU  - Jo MG
AD  - Division of Life Science and Applied Life Science (BK21 plus), College of Natural 
      Sciences, Gyeongsang National University (GNU), Jinju, 52802, Republic of Korea.
FAU - Choi, Myeong Jun
AU  - Choi MJ
AD  - Research and Development Center, Phytos Inc, Anyang mega valley 609, 268, Anyang, 
      Gyeonggi-do, Republic of Korea.
FAU - Kim, Myeong Ok
AU  - Kim MO
AD  - Division of Life Science and Applied Life Science (BK21 plus), College of Natural 
      Sciences, Gyeongsang National University (GNU), Jinju, 52802, Republic of Korea. 
      mokim@gnu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190912
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Biomarkers)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Oligonucleotides)
RN  - 0 (Reagent Kits, Diagnostic)
SB  - IM
MH  - *Alzheimer Disease/diagnostic imaging/genetics/metabolism
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Cell Line
MH  - Disease Models, Animal
MH  - Female
MH  - Fluorescent Dyes/*chemistry
MH  - Mice
MH  - Mice, Knockout, ApoE
MH  - Nanoparticles/*chemistry
MH  - Oligonucleotides/*chemistry
MH  - *Optical Imaging
MH  - *Reagent Kits, Diagnostic
PMC - PMC6742761
COIS- The authors declare no competing interests.
EDAT- 2019/09/14 06:00
MHDA- 2020/10/22 06:00
PMCR- 2019/09/12
CRDT- 2019/09/14 06:00
PHST- 2018/12/05 00:00 [received]
PHST- 2019/08/16 00:00 [accepted]
PHST- 2019/09/14 06:00 [entrez]
PHST- 2019/09/14 06:00 [pubmed]
PHST- 2020/10/22 06:00 [medline]
PHST- 2019/09/12 00:00 [pmc-release]
AID - 10.1038/s41598-019-49711-y [pii]
AID - 49711 [pii]
AID - 10.1038/s41598-019-49711-y [doi]
PST - epublish
SO  - Sci Rep. 2019 Sep 12;9(1):13184. doi: 10.1038/s41598-019-49711-y.