PMID- 31519771
OWN - NLM
STAT- MEDLINE
DCOM- 20200928
LR  - 20231014
IS  - 1573-4935 (Electronic)
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 39
IP  - 10
DP  - 2019 Oct 30
TI  - MiR-182 regulates cell proliferation and apoptosis in laryngeal squamous cell 
      carcinoma by targeting the CRR9.
LID - BSR20191348 [pii]
LID - 10.1042/BSR20191348 [doi]
AB  - BACKGROUND: The effect of miR-182 on the expressions of CRR9 in laryngeal 
      squamous cell carcinoma (LSCC) cells, and the impact on invasion and metastasis 
      of LSCC were investigated in the present paper. METHODS: The expressions of 
      miR-182 in LSCC tissue and cell line were detected by RT-qPCR. MTT assay and 
      Annexin V staining were used to detect the effects of miR-182 on tumor cells 
      proliferation. Target gene prediction and screening, and luciferase reporter 
      assay were designed to verify downstream target genes of miR-182. The mRNA and 
      protein expressions of CRR9 were detected by qRT-PCR and Western blot. Finally, 
      the expressions of CRR9 were measured by transfecting cells with miR-182 in mice. 
      RESULTS: Compared with normal tissue and cell, the expressions of miR-182 in 
      tumor tissues and cells were much lower. Over-expressions of miR-182 can increase 
      apoptosis rate. Luciferase reporter assay revealed that CRR9 was a downstream 
      gene of miR-182. Reintroduction of CRR9 abolished miR-182-induced LSCC cell 
      growth inhibition. In animal models, over-expressions of miR-182 can reduce tumor 
      weight and promote apoptosis. CONCLUSION: miR-182 can inhibit the proliferation 
      of LSCC cells by directly inhibiting the expressions of CRR9, thereby suppressing 
      the occurrences and developments of LSCC.
CI  - (c) 2019 The Author(s).
FAU - Lv, Yuan
AU  - Lv Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Li Huili Hospital 
      affiliated to Ningbo University, Ningbo City 315040, Zhejiang Province, P.R. 
      China.
FAU - Ye, Dong
AU  - Ye D
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Li Huili Hospital 
      affiliated to Ningbo University, Ningbo City 315040, Zhejiang Province, P.R. 
      China.
FAU - Qiu, Shijie
AU  - Qiu S
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Li Huili Hospital 
      affiliated to Ningbo University, Ningbo City 315040, Zhejiang Province, P.R. 
      China.
FAU - Zhang, Jian
AU  - Zhang J
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Li Huili Hospital 
      affiliated to Ningbo University, Ningbo City 315040, Zhejiang Province, P.R. 
      China.
FAU - Shen, Zhisen
AU  - Shen Z
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Li Huili Hospital 
      affiliated to Ningbo University, Ningbo City 315040, Zhejiang Province, P.R. 
      China.
FAU - Shen, Yi
AU  - Shen Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Li Huili Hospital 
      affiliated to Ningbo University, Ningbo City 315040, Zhejiang Province, P.R. 
      China.
FAU - Deng, Hongxia
AU  - Deng H
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Li Huili Hospital 
      affiliated to Ningbo University, Ningbo City 315040, Zhejiang Province, P.R. 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Retracted Publication
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (3' Untranslated Regions)
RN  - 0 (CLPTM1L protein, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn182 microRNA, human)
RN  - 0 (Neoplasm Proteins)
SB  - IM
RIN - Biosci Rep. 2022 Aug 31;42(8):. PMID: 36043420
MH  - 3' Untranslated Regions/genetics
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Carcinoma, Squamous Cell/*genetics/metabolism/therapy
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*genetics
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - HEK293 Cells
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/metabolism/therapy
MH  - Membrane Proteins/*genetics/metabolism
MH  - Mice, Nude
MH  - Mice, SCID
MH  - MicroRNAs/*genetics
MH  - Neoplasm Proteins/*genetics/metabolism
MH  - RNAi Therapeutics/methods
MH  - Tumor Burden/genetics
MH  - Xenograft Model Antitumor Assays/methods
PMC - PMC6822501
OTO - NOTNLM
OT  - CRR9
OT  - apoptosis
OT  - laryngeal squamous cell carcinoma
OT  - miR-182
OT  - proliferation
COIS- The study agreed with the Ethics Committee of Li huili hospital affiliated to 
      Ningbo University. Experiments using human materials were performed in strict 
      accordance with Declaration of Helsinki. Informed consent was signed for all 
      patients. The authors declare there are no competing interests associated with 
      the manuscript.
EDAT- 2019/09/15 06:00
MHDA- 2020/09/29 06:00
PMCR- 2019/10/11
CRDT- 2019/09/15 06:00
PHST- 2019/06/15 00:00 [received]
PHST- 2019/08/12 00:00 [revised]
PHST- 2019/09/01 00:00 [accepted]
PHST- 2019/09/15 06:00 [pubmed]
PHST- 2020/09/29 06:00 [medline]
PHST- 2019/09/15 06:00 [entrez]
PHST- 2019/10/11 00:00 [pmc-release]
AID - BSR20191348 [pii]
AID - 10.1042/BSR20191348 [doi]
PST - ppublish
SO  - Biosci Rep. 2019 Oct 30;39(10):BSR20191348. doi: 10.1042/BSR20191348.