PMID- 31521883
OWN - NLM
STAT- MEDLINE
DCOM- 20200318
LR  - 20200318
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 141
DP  - 2020 Jan 1
TI  - Trehalose supplementation during porcine oocytes in vitro maturation improves the 
      developmental capacity of parthenotes.
PG  - 91-97
LID - S0093-691X(19)30391-7 [pii]
LID - 10.1016/j.theriogenology.2019.09.009 [doi]
AB  - Autophagy is a critical process in early mammalian embryogenesis. Mammalian 
      target of rapamycin (mTOR) inhibitors are major regulators of autophagy. However, 
      mTOR plays a vital role in major signaling pathways controlling cell growth and 
      metabolism; thus, more secure autophagy activation methods should be considered. 
      The present study investigated the effects of supplementary trehalose, a novel 
      mTOR-independent autophagy enhancer, on oocyte maturation and embryonic 
      development after parthenogenetic activation (PA). Trehalose treatment during 
      in vitro maturation (IVM) did not affect the nuclear maturation rates of oocytes. 
      Oocytes treated with 25 mM trehalose during IVM had a significantly higher 
      (P < 0.05) blastocyst formation rate (64.2%) after PA compared to that in control 
      oocytes (52.0%). Blastocyst quality was also improved in the trehalose-treated 
      group. The total cell numbers for blastocyst formation and expanded blastocyst 
      formation were significantly increased in the trehalose-treated group (52.2% and 
      27.7%, respectively) compared to those in the control group (36.9% and 11.0%, 
      respectively). Trehalose treatment led to the increased expression of LC3, an 
      autophagy marker, in metaphase II oocytes and 4-cell stage embryos. Gene 
      expression analysis revealed that the expression of several autophagy related 
      genes (LAMP2, pATG5, and LC3) increased, while the Bax/Bcl2 ratio and 
      pro-apoptotic Bak transcript levels were decreased in the trehalose-treated 
      group. In conclusion, these results indicate that treatment with trehalose during 
      IVM improved the developmental potential of porcine embryos by down-regulation of 
      pro-apoptotic genes and up-regulation of autophagy-related genes and marker. 
      Trehalose may be useful for the large-scale production of high-quality porcine 
      blastocysts in vitro.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Cai, Lian
AU  - Cai L
AD  - Sooam Biotech Research Foundation, Seoul, 08359, Republic of Korea; Institute for 
      Stem Cell and Regenerative Medicine (ISCRM), College of Veterinary Medicine, 
      Chungbuk National University, Cheongju, 28644, Republic of Korea; Laboratory of 
      Veterinary Embryology and Biotechnology (VETEMBIO), College of Veterinary 
      Medicine, Chungbuk National University, Cheongju, 28644, Republic of Korea.
FAU - Jeong, Yeon-Woo
AU  - Jeong YW
AD  - Sooam Biotech Research Foundation, Seoul, 08359, Republic of Korea.
FAU - Hyun, Sang-Hwan
AU  - Hyun SH
AD  - Institute for Stem Cell and Regenerative Medicine (ISCRM), College of Veterinary 
      Medicine, Chungbuk National University, Cheongju, 28644, Republic of Korea; 
      Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), College of 
      Veterinary Medicine, Chungbuk National University, Cheongju, 28644, Republic of 
      Korea.
FAU - Yu, Il-Jeoung
AU  - Yu IJ
AD  - Department of Theriogenology and Reproductive Biotechnology, College of 
      Veterinary Medicine and Bio-safety Research Institute, Chonbuk National 
      University, Iksan, 54596, Republic of Korea.
FAU - Hwang, Woo-Suk
AU  - Hwang WS
AD  - Sooam Biotech Research Foundation, Seoul, 08359, Republic of Korea.
FAU - Jeon, Yubyeol
AU  - Jeon Y
AD  - Sooam Biotech Research Foundation, Seoul, 08359, Republic of Korea; Department of 
      Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine and 
      Bio-safety Research Institute, Chonbuk National University, Iksan, 54596, 
      Republic of Korea. Electronic address: ybjeon@jbnu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20190905
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - B8WCK70T7I (Trehalose)
SB  - IM
MH  - Animals
MH  - Blastocyst/drug effects/metabolism
MH  - Cumulus Cells/drug effects/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Embryo Culture Techniques/veterinary
MH  - Embryonic Development/drug effects/physiology
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - In Vitro Oocyte Maturation Techniques/*veterinary
MH  - Oocytes/*drug effects/physiology
MH  - *Parthenogenesis
MH  - *Swine
MH  - Trehalose/administration & dosage/*pharmacology
OTO - NOTNLM
OT  - Autophagy
OT  - Oocytes
OT  - Porcine
OT  - Trehalose
EDAT- 2019/09/16 06:00
MHDA- 2020/03/19 06:00
CRDT- 2019/09/16 06:00
PHST- 2019/04/04 00:00 [received]
PHST- 2019/08/20 00:00 [revised]
PHST- 2019/09/05 00:00 [accepted]
PHST- 2019/09/16 06:00 [pubmed]
PHST- 2020/03/19 06:00 [medline]
PHST- 2019/09/16 06:00 [entrez]
AID - S0093-691X(19)30391-7 [pii]
AID - 10.1016/j.theriogenology.2019.09.009 [doi]
PST - ppublish
SO  - Theriogenology. 2020 Jan 1;141:91-97. doi: 10.1016/j.theriogenology.2019.09.009. 
      Epub 2019 Sep 5.